Services
- Genetic Marker Assisted Breeding
- Plant Molecular Biology Research
- Research on DNA Level of Plant
- Research on Microspore Embryogenesis
- Plant Genetic Engineering
- Plant Genome Editing with CRISPR / Cas9
- Plant Genome Editing with TALEN
- Plant Genome Editing with ZFN
- RNAi Mediated Plant Gene Silencing
- Overexpression of Plant Genes
- Arabidopsis thaliana Transformation
- Oryza sativa Transformation
- Zea mays Transformation
- Triticum aestivum Transformation
- Medicago truncatula Transformation
- Glycine max Transformation
- Gossypium hirsutum Transformation
- Nicotiana tabacum Transformation
- Solanum lycopersicum Transformation
- Brassica napus Transformation
- Solanum tuberosum Transformation
- Virus-Induced Gene Silencing (VIGS) of Plant Genes
- Research on Protein Level of Plant
- Plant Epigenetic Modification Testing Services
- Sequencing-based Plant Breeding
- Plant DNA-level Sequencing Services
- Plant Genome De Novo Service
- Plant Whole Genome Resequencing Service
- Plant Reduced-Representation Genome Sequencing (RRGS)
- Plant Genetic Map Service
- BSA Trait Positioning of Plant
- Genome-Wide Association Study (GWAS) of Plant
- eQTL Analysis of Plant
- Plant Genetic Evolution Service
- Plant Pan-genome Sequencing
- Plant Whole Exome Sequencing Service
- Individual Selection Pressure Analysis of Plant
- Mixing-tank Selection Pressure Analysis of Plant
- Plant Whole Genome Survey
- Plant RNA Level Sequencing Services
- Eukaryotic Transcriptome Sequencing without Reference Genome
- Eukaryotic Transcriptome Sequencing with Reference Genome
- Prokaryotic Transcriptome Sequencing Analysis
- LncRNA Sequencing of Plant
- Plant Small RNA Sequencing
- Plant Circular RNA Sequencing
- Plant Comparative Transcriptome Service
- Plant Isoform-sequencing with Reference Genome
- Plant Isoform-sequencing without Reference Genome
- Ribo-seq of Plant
- Metatranscriptome Sequencing of Plant
- Plant Single Cell Level Sequencing Services
- Plant Epigenetics Level Sequencing Services
- Plant Proteomics Service
- Proteomics Qualitative Analysis in Plant
- Plant Protein Quantitative Analysis Service Based on Isotope Labeling (iTRAQ / TMT)
- Non-labeled Plant Protein Quantitative Analysis (Label-free / DIA)
- Plant Protein Targeted Quantitative Service (PRM / MRM / AQUA)
- Post-translational Modification Proteomics (PTMs) Service for Plant
- Plant Metabolomics Services
- Plant DNA-level Sequencing Services
- Other Services
- Plant CNV Analysis Service
- Plant Mutation Detection Service
- Plant Strain / Cell Level Services
- Plant Tissue and Cell Culture Services
- Plant Polyploidization Services
- Plant Haploidization Services
- Plant Phenotypic Analysis
- Plant Stress Response Indicators Analysis
- Plant Biochemical Analysis
- Plant Tissue and Cell Imaging Services
- Plant Disease Identification Services
- Plant Organelle Isolation Services
- Genetically Modified Plant Testing Services
- Seed Testing Services
Widely Targeted Metabolomics Service in Plant
INQUIRYIntroductions
Widely targeted metabolome is a metabolome detection technology that integrates the advantages of non-targeted and targeted metabolite detection technologies. It uses high-sensitivity LC/MS and a rich plant metabolite database to achieve high throughput, Highly sensitive, wide coverage, qualitative and accurate metabolite detection. The widely targeted metabolome can compare the types and abundance differences of metabolites between different samples, screen out significant differences in metabolites with important biological significance between different groups, and qualitatively and quantitatively detect low-abundance trace-level compounds. This technology is mainly used in plant growth and development research, plant stress biology research, crop agronomic traits research, active ingredient identification of medicinal plants, and nutrient color metabolism research of vegetables, fruits and flowers, etc.
Services
Plant widely targeted metabolome database
- Rich in material
- complete in variety
The widely target metabolome database that we used includes more than 6,000 substances, and covering more than 16 categories of metabolites. Such as flavonoids, phenolic acids, alkaloids, terpenes, quinones, steroids, tannins, vitamins, organic acids, amino acids, nucleotides, sugars and alcohols, lipids, lignans, coumarin, others.
Workflow of widely targeted metabolome in plant
Analysis content
| Standard information analysis | Raw data preprocessing (filtering, de-redundancy, peak identification, peak area integration, integration correction) |
| Metabolite identification | |
| Metabolite quantification | |
| PCA analysis | |
| (O)PLS-DA analysis | |
| Differential metabolite screening | |
| KEGG pathway enrichment analysis of differential metabolites | |
| Customized information analysis | Metabolite regulatory network analysis |
| Multi-omics association analysis (relevant data of other omics: transcriptome, proteome, genome, etc.) |
Sample requirements
- Roots, stems, leaves, fruits, seeds in plants> 200mg fresh weight/sample.
- Store at -80°C after quick freezing in liquid nitrogen. Shipped with dry ice.
- It is recommended that each experimental treatment has more than 6 biological replicates
For other sample types, please consult technical support or sales
Project cycle
- It takes 60~90 working days to complete the standard process.
Feedback to customers
- Raw data
- A project report includes experimental procedures, related mass spectrometry parameters, partial mass spectrometry pictures, raw data, and metabolomics analysis results
Service process
Advantages
- Lifeasible specializes in providing services related to agricultural molecular breeding, with standardized systems and scientific management.
- The experiment adopts a carefully optimized experimental procedure, which has extremely high efficiency and specificity.
- The one-stop service we provide makes your upstream and downstream experiments seamlessly connected, facilitates your experiments more conveniently and quickly.
- With a strong bioinformatics team, it can meet the various in-depth data analysis needs of customers.
- Personalized customized package service.
※ For research or industrial use.
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