Widely Targeted Metabolomics Service in Plant

INQUIRY

Introductions

Widely targeted metabolome is a metabolome detection technology that integrates the advantages of non-targeted and targeted metabolite detection technologies. It uses high-sensitivity LC/MS and a rich plant metabolite database to achieve high throughput, Highly sensitive, wide coverage, qualitative and accurate metabolite detection. The widely targeted metabolome can compare the types and abundance differences of metabolites between different samples, screen out significant differences in metabolites with important biological significance between different groups, and qualitatively and quantitatively detect low-abundance trace-level compounds. This technology is mainly used in plant growth and development research, plant stress biology research, crop agronomic traits research, active ingredient identification of medicinal plants, and nutrient color metabolism research of vegetables, fruits and flowers, etc.

Services

Plant widely targeted metabolome database

• Rich in material
• complete in variety

The widely target metabolome database that we used includes more than 6,000 substances, and covering more than 16 categories of metabolites. Such as flavonoids, phenolic acids, alkaloids, terpenes, quinones, steroids, tannins, vitamins, organic acids, amino acids, nucleotides, sugars and alcohols, lipids, lignans, coumarin, others.

Workflow of widely targeted metabolome in plant

Analysis content

Standard information analysis	Raw data preprocessing (filtering, de-redundancy, peak identification, peak area integration, integration correction)
	Metabolite identification
	Metabolite quantification
	PCA analysis
	(O)PLS-DA analysis
	Differential metabolite screening
	KEGG pathway enrichment analysis of differential metabolites
Customized information analysis	Metabolite regulatory network analysis
	Multi-omics association analysis (relevant data of other omics: transcriptome, proteome, genome, etc.)

Sample requirements

• Roots, stems, leaves, fruits, seeds in plants> 200mg fresh weight/sample.

For other sample types, please consult technical support or sales

• Store at -80°C after quick freezing in liquid nitrogen. Shipped with dry ice.
• It is recommended that each experimental treatment has more than 6 biological replicates

Project cycle

• It takes 60~90 working days to complete the standard process.

Feedback to customers

• Raw data
• A project report includes experimental procedures, related mass spectrometry parameters, partial mass spectrometry pictures, raw data, and metabolomics analysis results

Service process

Advantages

• Lifeasible specializes in providing services related to agricultural molecular breeding, with standardized systems and scientific management.
• The experiment adopts a carefully optimized experimental procedure, which has extremely high efficiency and specificity.
• The one-stop service we provide makes your upstream and downstream experiments seamlessly connected, facilitates your experiments more conveniently and quickly.
• With a strong bioinformatics team, it can meet the various in-depth data analysis needs of customers.
• Personalized customized package service.

 

※ It should be noted that our service is only used for research.

Related Sections

• Non-targeted Metabolomics Service in Plant
• Targeted Metabolomics Service in Plant
• Lipidomics Service in Plant

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