Services
- Genetic Marker Assisted Breeding
- Plant Molecular Biology Research
- Research on DNA Level of Plant
- Research on Microspore Embryogenesis
- Plant Genetic Engineering
- Plant Genome Editing with CRISPR / Cas9
- Plant Genome Editing with TALEN
- Plant Genome Editing with ZFN
- RNAi Mediated Plant Gene Silencing
- Overexpression of Plant Genes
- Arabidopsis thaliana Transformation
- Oryza sativa Transformation
- Zea mays Transformation
- Triticum aestivum Transformation
- Medicago truncatula Transformation
- Glycine max Transformation
- Gossypium hirsutum Transformation
- Nicotiana tabacum Transformation
- Solanum lycopersicum Transformation
- Brassica napus Transformation
- Solanum tuberosum Transformation
- Virus-Induced Gene Silencing (VIGS) of Plant Genes
- Research on Protein Level of Plant
- Plant Epigenetic Modification Testing Services
- Sequencing-based Plant Breeding
- Plant DNA-level Sequencing Services
- Plant Genome De Novo Service
- Plant Whole Genome Resequencing Service
- Plant Reduced-Representation Genome Sequencing (RRGS)
- Plant Genetic Map Service
- BSA Trait Positioning of Plant
- Genome-Wide Association Study (GWAS) of Plant
- eQTL Analysis of Plant
- Plant Genetic Evolution Service
- Plant Pan-genome Sequencing
- Plant Whole Exome Sequencing Service
- Individual Selection Pressure Analysis of Plant
- Mixing-tank Selection Pressure Analysis of Plant
- Plant Whole Genome Survey
- Plant RNA Level Sequencing Services
- Eukaryotic Transcriptome Sequencing without Reference Genome
- Eukaryotic Transcriptome Sequencing with Reference Genome
- Prokaryotic Transcriptome Sequencing Analysis
- LncRNA Sequencing of Plant
- Plant Small RNA Sequencing
- Plant Circular RNA Sequencing
- Plant Comparative Transcriptome Service
- Plant Isoform-sequencing with Reference Genome
- Plant Isoform-sequencing without Reference Genome
- Ribo-seq of Plant
- Metatranscriptome Sequencing of Plant
- Plant Single Cell Level Sequencing Services
- Plant Epigenetics Level Sequencing Services
- Plant Proteomics Service
- Proteomics Qualitative Analysis in Plant
- Plant Protein Quantitative Analysis Service Based on Isotope Labeling (iTRAQ / TMT)
- Non-labeled Plant Protein Quantitative Analysis (Label-free / DIA)
- Plant Protein Targeted Quantitative Service (PRM / MRM / AQUA)
- Post-translational Modification Proteomics (PTMs) Service for Plant
- Plant Metabolomics Services
- Plant DNA-level Sequencing Services
- Other Services
- Plant CNV Analysis Service
- Plant Mutation Detection Service
- Plant Strain / Cell Level Services
- Plant Tissue and Cell Culture Services
- Plant Polyploidization Services
- Plant Haploidization Services
- Plant Phenotypic Analysis
- Plant Stress Response Indicators Analysis
- Plant Biochemical Analysis
- Plant Tissue and Cell Imaging Services
- Plant Disease Identification Services
- Plant Organelle Isolation Services
- Genetically Modified Plant Testing Services
- Seed Testing Services
Plant Isoform-sequencing without Reference Genome
INQUIRYIntroductions
PacBio's third-generation sequencing (Isoform-sequencing) uses single-molecule real-time sequencing technology (SMRT). Due to its ultra-long read length (average 15kb), complete full-length transcripts can be obtained directly without splicing, so higher quality transcripts can be obtained, which is conducive to the study of mRNA structure, such as variable splicing and fusion genes, Allele expression, etc. Overcome the shortcomings of the second-generation sequencing technology due to the short reads length. At present, full-length transcriptome sequencing technology has been widely used in plant research, including Arabidopsis, corn, wheat, sorghum, salvia, petunia, etc. Full-length transcriptome sequencing without reference genomes can be used to study the acquisition of more complete reference coding sequences of species without reference genomes, to study changes in variable splicing events caused by different experimental treatments, and more accurate quantitative analysis, etc.
Services
Based on PacBio's third-generation sequencing platform, Lifeasible uses single molecule real-time sequencing technology (SMRT) to provide professional isoform sequencing and analysis services without reference genome to help your research.
Workflow of isoform sequencing and analysis services without reference genome
Analysis content
| Standard infromation analysis | Statistics and quality control of raw sequencing data |
| Full-length isoform data statistics | |
| Basic function annotation of genes a) Nr annotation b) GO function annotation c) COG/KOG annotatio d) KEGG metabolic pathway annotation e) SwissProt protein annotation | |
| Advanced functional annotation of genes a) Predicted coding protein frame(CDS) b) Transcription factor analysis c) R gene analysis (plants) d) Protein domain analysis(Pfam, SMART) e) TMHMM transmembrane spiral structure prediction f) Signal peptide structure prediction g) Protein O-GlcNAc glycosylation site prediction(mammalian) h) ProP furin cleavage site prediction (eukaryote) | |
| Structural analysis a) Tandem repeat unit detection(SSR) b) InCRNA analysis c) Alternative splicing analysis | |
| Customized information analysis | Correction of the second-generation data to the third-generation data(illumina data required) |
| Quantitative and differential expression analysis of genes(illumina data required) | |
| Multi-omics association analysis (such as methylation, proteomics, miRNA) |
Sample requirements
- Sample size
- Sample purity
- RNA integrity
- Please put the sample in a 1.5ml tube, indicate the sample name, concentration and preparation time on the tube, and use parafilm to seal the tube mouth.
- Before shipping, fix all sample tubes in 50ml centrifuge tubes with caps and ship them on dry ice.
Total RNA>12μg (2 libraries)
OD260/280≥1.8, OD260/230≥1.0. And there is a normal peak at 260nm.
RIN≥8.0, 28S/18S≥1.3, the baseline of the spectrum is not raised, and the 5S peak is normal.
Project cycle
- The entire project generally takes about 2 months.
Feedback to customers
- Raw data
- A project report includes experimental process, instrument parameters, analysis methods and results, etc.
Service process
Advantages
- Lifeasible specializes in providing services related to agricultural molecular breeding, with standardized systems and scientific management.
- PacBio's third-generation sequencer has the advantages of ultra-long read length, high throughput, no GC preference, and direct detection of base modifications, etc.
- The one-stop service we provide makes your upstream and downstream experiments seamlessly connected, facilitates your experiments more conveniently and quickly.
- The continuously updated and expanded high-performance computing platform ensures efficient data processing and secure data storage.
- Personalized customized package service.
※ For research or industrial use.
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