Services
- Genetic Marker Assisted Breeding
- Plant Molecular Biology Research
- Research on DNA Level of Plant
- Research on Microspore Embryogenesis
- Plant Genetic Engineering
- Plant Genome Editing with CRISPR / Cas9
- Plant Genome Editing with TALEN
- Plant Genome Editing with ZFN
- RNAi Mediated Plant Gene Silencing
- Overexpression of Plant Genes
- Arabidopsis thaliana Transformation
- Oryza sativa Transformation
- Zea mays Transformation
- Triticum aestivum Transformation
- Medicago truncatula Transformation
- Glycine max Transformation
- Gossypium hirsutum Transformation
- Nicotiana tabacum Transformation
- Solanum lycopersicum Transformation
- Brassica napus Transformation
- Solanum tuberosum Transformation
- Virus-Induced Gene Silencing (VIGS) of Plant Genes
- Research on Protein Level of Plant
- Plant Epigenetic Modification Testing Services
- Sequencing-based Plant Breeding
- Plant DNA-level Sequencing Services
- Plant Genome De Novo Service
- Plant Whole Genome Resequencing Service
- Plant Reduced-Representation Genome Sequencing (RRGS)
- Plant Genetic Map Service
- BSA Trait Positioning of Plant
- Genome-Wide Association Study (GWAS) of Plant
- eQTL Analysis of Plant
- Plant Genetic Evolution Service
- Plant Pan-genome Sequencing
- Plant Whole Exome Sequencing Service
- Individual Selection Pressure Analysis of Plant
- Mixing-tank Selection Pressure Analysis of Plant
- Plant Whole Genome Survey
- Plant RNA Level Sequencing Services
- Eukaryotic Transcriptome Sequencing without Reference Genome
- Eukaryotic Transcriptome Sequencing with Reference Genome
- Prokaryotic Transcriptome Sequencing Analysis
- LncRNA Sequencing of Plant
- Plant Small RNA Sequencing
- Plant Circular RNA Sequencing
- Plant Comparative Transcriptome Service
- Plant Isoform-sequencing with Reference Genome
- Plant Isoform-sequencing without Reference Genome
- Ribo-seq of Plant
- Metatranscriptome Sequencing of Plant
- Plant Single Cell Level Sequencing Services
- Plant Epigenetics Level Sequencing Services
- Plant Proteomics Service
- Proteomics Qualitative Analysis in Plant
- Plant Protein Quantitative Analysis Service Based on Isotope Labeling (iTRAQ / TMT)
- Non-labeled Plant Protein Quantitative Analysis (Label-free / DIA)
- Plant Protein Targeted Quantitative Service (PRM / MRM / AQUA)
- Post-translational Modification Proteomics (PTMs) Service for Plant
- Plant Metabolomics Services
- Plant DNA-level Sequencing Services
- Other Services
- Plant CNV Analysis Service
- Plant Mutation Detection Service
- Plant Strain / Cell Level Services
- Plant Tissue and Cell Culture Services
- Plant Polyploidization Services
- Plant Haploidization Services
- Plant Phenotypic Analysis
- Plant Stress Response Indicators Analysis
- Plant Biochemical Analysis
- Plant Tissue and Cell Imaging Services
- Plant Disease Identification Services
- Plant Organelle Isolation Services
- Genetically Modified Plant Testing Services
- Seed Testing Services
Plant Polyploidization Services
INQUIRYIntroductions
Polyploidization plays a key role in plant Breeding and crop improvement. Polyploidy originates mainly through three ways: unreduced gamete fusion, somatic chromosome doubling, and polyspermia fertilization. Among them, unreduced gametes are the main mechanism of polyploid formation. Polyploids are also important as bridges for genetic transfer between species of hybrid incompatibility, allowing the creation of plant varieties with enhanced pest resistance and stress tolerance. Polyploidy also gives plant breeders more options for developing plant variants with desired traits. Polyploidy and genome-wide replication can cause gene recombination, gene mutation, transposon activity and chromosome rearrangement, and can also drive the evolution and differentiation of plant behavior and gene function, thereby achieving diversification.
Schematic representation of four cultivated species and some of the main polyploidy consequences for application in crop improvement.
The symbols "n 1x" and "n 2x" refer to reduced and unreduced reproductive cells, respectively (picture from Sattler M C et al., 2016)
Services
As a leader in the field of plant biotechnology, Lifeasible has developed an advanced platform that can induce polyploidy in a variety of crops.
Plant polyploidization methods
- Chemical reagent induction
- Protoplast fusion method
1. Colchicine
It can prevent chromosomes / chromatids from multiplying in later stages and cause chromosomes to double by inhibiting microtubule polymerization.
2. Anti-mitotic chemicals
Such as trifluralin, aspartame, methamphetamine, bufaphos and N2O gas.
It is a biological culture technology that can adhere and fuse two or more protoplasts under the induction of polyethylene glycol (PEG) or in the case of electric shock.
Plant protoplasts, mature or immature embryos, leaves, hypocotyls, roots, etc.
Sample requirements
- Plant tissue or cell
- Plant variety name
- Plant materials
Delivery standard
- Transplanted plants or other forms.
- Raw images.
- Complete experimental report, including methods, original experimental records, instrument parameters, reagents and consumables information.
Service process
Project cycle
It takes about 2~3 months from sample delivery. The specific time required for different plant spices is different, please contact us for details.
Advantages
- We have built a professional platform for plant polyploidization, and we have also mastered 2 methods to meet your needs.
- Our experienced scientists and experts will wholeheartedly provide you with assistance in project design, experiment optimization, data interpretation, technical consultation, etc.
- The one-stop service we provide makes your upstream and downstream experiments seamlessly connected, facilitates your experiments more conveniently and quickly.
- Standardization system, scientific management.
Reference
- Sattler M C, Carvalho C R, Clarindo W R. The polyploidy and its key role in plant Breeding[J]. Planta, 2016, 243(2):281-296.
※ It should be noted that our service is only used for research.
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