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Lodging-Resistant Barley Breeding Service

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Why Breed Lodging-Resistant Barley?

Barley (Hordeum vulgare L.) is a very major food crop that ranks fourth in the world for both grain yield and area under cultivation. Currently, barley is widely used for making beer, human consumption, and animal feed. The habit of barley to bend over and lie on the ground causes yield losses due to suboptimal grain-filling conditions and technical challenges in efficient grain harvest. Spikes and grains of lodged barley increase the chances for fungal infections and thereby mycotoxin accumulation, as well as preharvest germination. Today, cereal lodging is still in focus of commercial plant breeding. Molecular breeding for lodging-resistant in barley is one of the strategies to address these challenges. The selection of barley varieties that exhibit a certain level of lodging-resistant, which is benefits on barley yield and the production of high-quality fodder.

Breeding Services for Lodging-Resistant Barley

Traditional breeding shows disadvantages such as uncontrollable, long cycle and more interference, therefore, molecular biology technologies have emerged as a key breeding strategy for barley. The introduction of semi-dwarf mutations in barley was a breakthrough in preventing lodging. Lifeasible provides numbers of methods to accelerate the breeding process of lodging-resistant barley.

  • QTL Mapping: A candidate gene approach which is performed in constructed bi- parental populations with contrasting parents to detect various QTLs and then estimate its position and effect on the plant traits after introgression.
  • Gene Mapping: A method that the genetic markers are developed and a population is mapped to identify the distances between genes via analysis of co-segregation patterns and specific locus of a gene based solely on their phenotypic effect.
  • Marker-assisted Selection: A type of indirect selection for the desired plant phenotype which is based on the linked molecular markers banding pattern.
  • Tilling by sequencing (Tbys): A general reverse genetics strategy which includes mutagenesis of a large plant population followed by identification of point mutations in the gene of interest via high-throughput detection system.
  • Transgenics: biotechnological interventions, including genetic engineering, RNA silencing technologies, ad gene editing.

Allelism crosses.Figure 1. Allelism crosses. Phenotypic comparison between cultivar 'Bowman', mutant BW314 (ert-k.32), an F1 offspring from a cross BW314 (ert-k.32) × ert-k.32, mutant BW857 (sld.k) and an F1 offspring from a representative cross BW857 (sld.k) × ert-k.32. (Kristensen, P.S.; et al., 2016)

Advantages of Our Lodging-Resistant Barley Breeding Service

  • Barley-specific culture medium and transformation methods to ensure rapid and accurate transformation.
  • Extensive SOP operation specification and multiple experimental quality control (QC) procedures to improve transgenic efficiency.
  • Provide professional and personalized services to meet the needs of different customers.
  • Efficient transgenic system to obtain a large number of transgenic plants.

Lifeasible is committed to accelerating barley breeding for lodging-resistant varieties through molecular biology technology platforms. Please contact us if you have a related research need or would like more information about our services.

Reference

  1. Kristensen, P.S.; et al. Genetic mapping of the barley lodging resistance locus Erectoides-k. Plant Breed. 2016, 135: 420-428.

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