Services
- Genetic Marker Assisted Breeding
- Plant Molecular Biology Research
- Research on DNA Level of Plant
- Research on Microspore Embryogenesis
- Plant Genetic Engineering
- Plant Genome Editing with CRISPR / Cas9
- Plant Genome Editing with TALEN
- Plant Genome Editing with ZFN
- RNAi Mediated Plant Gene Silencing
- Overexpression of Plant Genes
- Arabidopsis thaliana Transformation
- Oryza sativa Transformation
- Zea mays Transformation
- Triticum aestivum Transformation
- Medicago truncatula Transformation
- Glycine max Transformation
- Gossypium hirsutum Transformation
- Nicotiana tabacum Transformation
- Solanum lycopersicum Transformation
- Brassica napus Transformation
- Solanum tuberosum Transformation
- Virus-Induced Gene Silencing (VIGS) of Plant Genes
- Research on Protein Level of Plant
- Plant Epigenetic Modification Testing Services
- Sequencing-based Plant Breeding
- Plant DNA-level Sequencing Services
- Plant Genome De Novo Service
- Plant Whole Genome Resequencing Service
- Plant Reduced-Representation Genome Sequencing (RRGS)
- Plant Genetic Map Service
- BSA Trait Positioning of Plant
- Genome-Wide Association Study (GWAS) of Plant
- eQTL Analysis of Plant
- Plant Genetic Evolution Service
- Plant Pan-genome Sequencing
- Plant Whole Exome Sequencing Service
- Individual Selection Pressure Analysis of Plant
- Mixing-tank Selection Pressure Analysis of Plant
- Plant Whole Genome Survey
- Plant RNA Level Sequencing Services
- Eukaryotic Transcriptome Sequencing without Reference Genome
- Eukaryotic Transcriptome Sequencing with Reference Genome
- Prokaryotic Transcriptome Sequencing Analysis
- LncRNA Sequencing of Plant
- Plant Small RNA Sequencing
- Plant Circular RNA Sequencing
- Plant Comparative Transcriptome Service
- Plant Isoform-sequencing with Reference Genome
- Plant Isoform-sequencing without Reference Genome
- Ribo-seq of Plant
- Metatranscriptome Sequencing of Plant
- Plant Single Cell Level Sequencing Services
- Plant Epigenetics Level Sequencing Services
- Plant Proteomics Service
- Proteomics Qualitative Analysis in Plant
- Plant Protein Quantitative Analysis Service Based on Isotope Labeling (iTRAQ / TMT)
- Non-labeled Plant Protein Quantitative Analysis (Label-free / DIA)
- Plant Protein Targeted Quantitative Service (PRM / MRM / AQUA)
- Post-translational Modification Proteomics (PTMs) Service for Plant
- Plant Metabolomics Services
- Plant DNA-level Sequencing Services
- Other Services
- Plant CNV Analysis Service
- Plant Mutation Detection Service
- Plant Strain / Cell Level Services
- Plant Tissue and Cell Culture Services
- Plant Polyploidization Services
- Plant Haploidization Services
- Plant Phenotypic Analysis
- Plant Stress Response Indicators Analysis
- Plant Biochemical Analysis
- Plant Tissue and Cell Imaging Services
- Plant Disease Identification Services
- Plant Organelle Isolation Services
- Genetically Modified Plant Testing Services
- Seed Testing Services
Gossypium hirsutum Transformation
INQUIRYIntroductions
Cotton is the seed fiber of gossypium plants of the Malvaceae family, and its scientific name is Gossypium hirsutum. It is not only important fiber crops, oil crops and high-protein food crops, but also textiles, fine chemical raw materials and important strategic materials. As the growth and development of cotton will be adversely affected by biotic and abiotic stresses such as virus and fungal infection, insect predation and drought stress, its yield and quality will decrease. With the deepening of molecular biology research and the gradual maturity of plant transgenic technology, genetic engineering can be used to genetically improve cotton. Genetic engineering technology can keep the other traits of the variety relatively stable, and only change the traits controlled by the foreign gene. For example, transgenic cotton plants expressing the Bt gene (a gene encoding Bt toxin) can produce natural insecticides to combat certain major pests, and have been successfully used in many countries.
Services
Lifeasible has established a cotton genetic transformation platform, providing a variety of transformation systems for researchers who use cotton as their research objects. We have an experienced team that can help you solve technical problems and help your project succeed.
Customer provided materials
- If only gene sequence information (or Gene Bank ID) is provided, our company can undertake gene amplification and vector construction services, which are charged separately.
- Construct a vector of Escherichia coli, plasmid or Agrobacterium, but provide clear resistance information for screening.
- Unconventional receptor material.
※ We do not accept the transformation service of lethal genes or severely defective genes. If there are any of the above, please inform our company in advance.
Transformation methods
- Agrobacterium-mediated transformation
- Biolistic particle delivery system
- Pollen tube pathway mediated transformation
- Silicon carbide whiskers -mediated transformation
Receptor material
- Recipient species
- Recipient types
Coker series, AcalaB1654, TM-1, Jin7, Zhongmian12, Narasimha, Si Mian3, etc.
Explants such as hypocotyls, cotyledonary petiole or shoot apex.
Embryogenic callus derived from hypocotyls, cotyledonary petiole, or cell suspension cultures.
Workflow of cotton transformation
Feedback to customers
- Complete experimental methods, original experimental records, instrument parameters, reagents and consumables information.
- Vector construction sequencing report and pictures.
- Pictures of explant infection, induced callus, embryogenic callus, tissue differentiation, plant regeneration, etc.
- Gel electrophoresis diagram of PCR detection of target genes in T0 generation regenerated plants
- T0 generation transgenic vaccine: If we use the vector and recipient provided by us, the T0 generation positive vaccine for each transformation experiment is ≥8; if the customer needs to use other vectors and recipients, we usually only promise to get the T0 generation positive vaccine 6-8 strains.
Service process
Project cycle
- It takes about 10~12 months from the construction of the vector to the test results of the T0 generation transgenic seedlings. And experiments can be carried out throughout the year.
Advantages
- We have 4 cotton genetic transformation methods to meet your different needs.
- We have a standard cotton differentiation and transformation system with stable emergence and high positive rate.
- We have a large-scale, standardized seedling system to ensure the success rate of experimental transformation.
- We have a variety of labeled vectors and media to provide you with a wide range of choices.
- Personalized customized package service.
- Perfect service support. Provide professional technical consultation and guidance in the early stage to optimize the project; communicate at any time during the experiment to keep abreast of the progress; after the project is completed, provide complete results according to the contract.
※ It should be noted that our service is only used for research.
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