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Breeding of Herbicide-Resistant Alfalfa
INQUIRYIntroduction
Alfalfa is a perennial forage with rich nutrition and strong adaptability to the environment. It is a widely distributed cultivated forage in the world today. However, due to the poor competitiveness of alfalfa, it is easy to be harmed by weeds in the field, which leads to the reduction of pasture yield and affects the quality of pasture. Herbicides are agents that can kill weeds completely or selectively, but they can also cause great harm to the growth of crop. In order to reduce the influence of herbicides on the growth of alfalfa, improving the variety of alfalfa is the most important. The screening of excellent herbicide-resistant transgenic materials can effectively solve the problem of weed control in alfalfa production, and provide more material basis for the promotion of forage industry.
Service
Traditional breeding methods of alfalfa have certain limitations and timeliness because of its slow growth, low self-crossing rate and less planting area. With the advent of transgenic crops, herbicide resistant transgenic crops have become a common requirement. Lifeasible provides several strategies to accelerate the breeding process of herbicide-resistant alfalfa.
- Transgenic Genetic Engineering
At present, alfalfa transgenic plants are mainly achieved through Agrobacterium-mediated genetic transformation pathway. In addition, gene gun method, direct conversion method, microinjection method and pollen tube channel method are also important methods for genetic transformation of alfalfa.
- Gene Editing Technology
The herbicide resistance genes of alfalfa were modified by CRISPR/Cas9 gene editing technology to obtain broad-spectrum and durable drug resistance.
Figure 1. Transgenic alfalfa plants obtained by A. tumefaciens-mediated transformation. (Yi D.; et al. 2018)
- High-Throughput Sequencing Technology
To understand the genetic mechanism of alfalfa resistance, DNA-level sequencing, RNA-level sequencing, single-cell level sequencing, epigenetic-level sequencing, proteomics and metabolomics analyses were performed.
Advantages
- Multiple advanced molecular biology platforms to provide different sources of gene transformation services to support your molecular breeding research
- Standardized management system and optimized experimental procedures to ensure efficient and accurate experimental results
- The professional technical team can customize the suitable genetic transformation program for you to meet the research needs of different customers and obtain the positive transformation seeds that can meet the downstream experiments
- Timely feedback and communication to ensure accurate implementation and repositioning of the agreement
- Experienced technical team, one-stop service, speed up the research process
Lifeasible provides a one-stop experimental experience from the construction of the vector to the acquisition of transgenic seedlings and related physiological and biochemical tests. If you have related research needs or would like to know more about our services, please feel free to contact us.
Reference
- Yi, D., et al. Development of glyphosate-resistant alfalfa (Medicago sativa L.) upon transformation with the GR79Ms gene encoding 5-enolpyruvylshikimate-3-phosphate synthase. Planta. 2018, 248(1): 211-219.
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