Services
- Genetic Marker Assisted Breeding
- Plant Molecular Biology Research
- Research on DNA Level of Plant
- Research on Microspore Embryogenesis
- Plant Genetic Engineering
- Plant Genome Editing with CRISPR / Cas9
- Plant Genome Editing with TALEN
- Plant Genome Editing with ZFN
- RNAi Mediated Plant Gene Silencing
- Overexpression of Plant Genes
- Arabidopsis thaliana Transformation
- Oryza sativa Transformation
- Zea mays Transformation
- Triticum aestivum Transformation
- Medicago truncatula Transformation
- Glycine max Transformation
- Gossypium hirsutum Transformation
- Nicotiana tabacum Transformation
- Solanum lycopersicum Transformation
- Brassica napus Transformation
- Solanum tuberosum Transformation
- Virus-Induced Gene Silencing (VIGS) of Plant Genes
- Research on Protein Level of Plant
- Plant Epigenetic Modification Testing Services
- Sequencing-based Plant Breeding
- Plant DNA-level Sequencing Services
- Plant Genome De Novo Service
- Plant Whole Genome Resequencing Service
- Plant Reduced-Representation Genome Sequencing (RRGS)
- Plant Genetic Map Service
- BSA Trait Positioning of Plant
- Genome-Wide Association Study (GWAS) of Plant
- eQTL Analysis of Plant
- Plant Genetic Evolution Service
- Plant Pan-genome Sequencing
- Plant Whole Exome Sequencing Service
- Individual Selection Pressure Analysis of Plant
- Mixing-tank Selection Pressure Analysis of Plant
- Plant Whole Genome Survey
- Plant RNA Level Sequencing Services
- Eukaryotic Transcriptome Sequencing without Reference Genome
- Eukaryotic Transcriptome Sequencing with Reference Genome
- Prokaryotic Transcriptome Sequencing Analysis
- LncRNA Sequencing of Plant
- Plant Small RNA Sequencing
- Plant Circular RNA Sequencing
- Plant Comparative Transcriptome Service
- Plant Isoform-sequencing with Reference Genome
- Plant Isoform-sequencing without Reference Genome
- Ribo-seq of Plant
- Metatranscriptome Sequencing of Plant
- Plant Single Cell Level Sequencing Services
- Plant Epigenetics Level Sequencing Services
- Plant Proteomics Service
- Proteomics Qualitative Analysis in Plant
- Plant Protein Quantitative Analysis Service Based on Isotope Labeling (iTRAQ / TMT)
- Non-labeled Plant Protein Quantitative Analysis (Label-free / DIA)
- Plant Protein Targeted Quantitative Service (PRM / MRM / AQUA)
- Post-translational Modification Proteomics (PTMs) Service for Plant
- Plant Metabolomics Services
- Plant DNA-level Sequencing Services
- Other Services
- Plant CNV Analysis Service
- Plant Mutation Detection Service
- Plant Strain / Cell Level Services
- Plant Tissue and Cell Culture Services
- Plant Polyploidization Services
- Plant Haploidization Services
- Plant Phenotypic Analysis
- Plant Stress Response Indicators Analysis
- Plant Biochemical Analysis
- Plant Tissue and Cell Imaging Services
- Plant Disease Identification Services
- Plant Organelle Isolation Services
- Genetically Modified Plant Testing Services
- Seed Testing Services
Plant Whole Exome Sequencing Service
INQUIRYINTRODUCTIONS
The exome is the sum of all exon regions in the genome of a species. Exome sequencing refers to the use of sequence capture technology to enrich the DNA of the exon region of the whole genome and then perform high-throughput sequencing. Through high-throughput sequencing, the technical means to discover genetic mutations related to protein functional variation. This method can obtain the genetic information of the designated exon capture platform probe design region and the flanking 200bp sequence, which greatly improves the research efficiency of the exon region in the genome and significantly reduces the research cost. It is mainly used to identify and study structural variations in coding regions related to disease and population evolution.
SERVICES
With years of rich experience, Lifeasible provides plant whole exome sequencing service. We provide a one-stop service model. We complete a series of processes from sample extraction, quality testing, sequencing, biological information analysis to making reports.
Technical route
Analysis content
Advanced analysis
- Screening based on the harmfulness of variants
1. Mutation site screening
2. Classification of Mutation Site Harmfulness (ACMG)
3. Harmfulness analysis of structural variation CNV
- Screening based on selection information
1. Analysis of dominant/recessive inheritance patterns (family diagrams are required)
2. pedigree linkage analysis (family sample) (single gene disease)
New mutation screening (core family) (complex diseases)
3. Homozygous region (ROH) analysis (single gene disease)
4. Screening of shared mutant genes (distributed samples)
- Screening based on gene function and phenotype
1. Candidate gene function enrichment analysis
2. Candidate gene pathway enrichment analysis
3. Candidate genes and disease relevance ranking
4. Relevance ranking of candidate genes and diseases (complex diseases)
Personalized analysis
- Study on the genetic mechanism of drug effect polymorphism
- Survival analysis (based on clinical follow-up data)
- Analysis of significant disease association sites (recommended based on more than 150 pairs of case/control)
- Analysis of significant disease-related genes (recommended based on more than 150 pairs of case/control)
- HLA analysis (analysis content refers to human HLA capture sequencing
- Analysis content)
Sample requirements
- Sample type: DNA sample without degradation and without protein and RNA contamination.
Total amount of sample ≥6μg, concentration >50ng/μl, OD260/280=1.8~2.0.
Project cycle
- The entire project generally takes about 2~3 months, and the specific completion time depends on the size and complexity of the genome.
Feedback to customers
- Raw data
- A project report includes experimental process, instrument parameters, analysis methods and results, etc.
Service process
ADVANTAGES
- Compared with whole-genome sequencing, exome sequencing is deeper, more economical, and more efficient. Directly sequence the protein coding sequence to find out the variation that affects the protein structure; High-depth sequencing can find common, low-frequency and rare mutations; Sequencing of exome regions accounts for about 1% of the genome, effectively reducing costs, cycles and workload.
- Lifeasible has a high-performance computing platform (HPC) that uses an efficient combination of DELL computing nodes and Isilon storage to achieve fast and stable sequencing data analysis and delivery.
- Personalized customized package service.
※ It should be noted that our service is only used for research.
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