Breeding of Drought- and Barren-tolerant Barley

INQUIRY

Introduction

Barley (Hordeum vulgare) ranks the fourth largest harvested cereal in the world with multiple uses as animal feed, human food, and brewing material. The nutritive and palatable value of barley has drawn increasing attention, along with interest in its potential for food breeding and exploitation. Drought and bareness are devastating environmental restrictions impacting barley agronomic productivity, leading to substantial declines in photosynthesis, floral abnormalities, spikelet and kernel sterility, grain yield reductions and grain of poor quality. Molecular breeding for drought- and barren-tolerant in barley is one of the strategies to address these challenges. The selection of barley varieties that exhibit a certain level of tolerance to drought and barren, which is benefits on barley yield and the production of high-quality fodder.

Service

Barley is an autogamous diploid crop, makes it a valuable material for wheat crop genome investigations. Traditional breeding shows disadvantages such as uncontrollable, long cycle and more interference, therefore, molecular biology technologies have emerged as a key breeding strategy for barley. Crop tolerance to drought and barren can be increased by introducing drought and barren tolerant related genes via genetic engineering techniques. Lifeasible provides numbers of methods to accelerate the breeding process of drought and barren tolerant barley.

• Genetic transformation of barley: Obtain transgenic plants of barley by Agrobacterium-mediated transformation and gene gun mediated transformation.
• Genome-wide association study: Identification of drought- and barren-tolerant genes in barley and combining with gene editing technology for breeding.
• Gene editing: Modification of barley tolerance-related genes by gene editing technology with a view to obtaining drought and barren tolerance.
• Analysis of drought and barren tolerance related genetic mechanism: To understand the drought and barren tolerance related genetic mechanism in barley through proteomics analysis.
• Molecular markers: Screening resistance-related molecular markers, constructing molecular marker profiles, locating drought and barren tolerance related genes, and characterizing genetic diversity.

Figure 1. Stress recovery in NIL-143 and Scarlett at the seedling stage greenhouse conditions. (Shrestha, A. et al., 2022)

Technology Platforms

• High-throughput sequencing technology platform: DNA-level sequencing, RNA-level sequencing, single cell level sequencing, epigenetics level sequencing, proteomics and metabolomics services.
• Genetic engineering technology platform: CRISPR / Cas9, transcription activator-like effector nucleases (TALENs), zinc finger nucleases (ZFNs), RNA Interference (RNAi), gene overexpression, virus-induced gene silencing (VIGS).
• Plant SNP Analysis: KASP, GWAS analysis, QTL analysis, direct sequencing, TaqMan probe, SNPSHOT, MassARRAY.

As an expert in botany and agronomy, Lifeasible is committed to accelerating plant breeding researches through molecular biology technology platforms. If you have a related research need or would like more information about our services, please feel free to contact us.

Reference

1. Shrestha, A.; et al. Natural diversity uncovers P5CS1 regulation and its role in drought stress tolerance and yield sustainability in barley. Plant, Cell & Environment. 2022, 45: 3523– 3536.

 

※ For research or industrial use.

Related Sections

• Early Maturing Barley Breeding Service
• Breeding for Barley with More Tillers
• Breeding for High Quality and High Yielding Barley
• Lodging-Resistant Barley Breeding Service

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