Plant Protein Subcellular Localization Services

INQUIRY

Introductions

Subcellular localization refers to the specific location of a certain protein or expression product in a cell, for example, in the nucleus, in the cytoplasm, or on the cell membrane. The study of protein subcellular localization is essential for the systematic understanding of plant morphogenesis, growth and development, and resistance to adversity. It is also an important content of genomics research. At present, the most commonly used method is to perform co-  localization observation through fusion of reporter genes. Among them, green fluorescent protein (GFP) is the most widely used reporter gene. This method must construct a fusion protein vector, label the target protein with GFP that functions as a reporter protein, and express it effectively after transfection. If a GFP signal is seen in a certain position in the cell under a fluorescence microscope, it means that the target protein fused with GFP is also expressed at that position, thereby determining the subcellular location of a certain substance.

Services

The subcellular localization analysis of plant protein is mainly through the construction of an overexpression plasmid fused with fluorescent protein, and then transient expression of tobacco or protoplasts to determine its localization and co-localization. Lifeasible provides scientists with convenient subcellular localization technology services, through the method of Agrobacterium-mediated infiltration tobacco or the method of PEG-mediated plasmid transfer into protoplasts, with no-load as a positive control, using a laser scanning confocal microscope for observation and take pictures.

Plant protein subcellular localization services

• Agrobacterium-mediated infiltration tobacco
• PEG-mediated plasmid transfer into protoplasts

Sample requirements

• Constructed expression vector plasmid (or provide sequence constructed by Lifeasible)
• Agrobacterium solution of constructed expression vector

Basic workflow of plant protein subcellular localization services

• Expression vector construction

Transform Agrobacterium and infect tobacco

PEG-mediated plasmid transformation into protoplasts

• Laser scanning confocal microscope observation and photography
• Organize experiment report

Delivery standard

• Project report

Electronic word document

• Images

Electronic version of pictures in JPG or TIF format

Service process

• Service inquiry and technical consultation
• Design experiment
• Sign contracts and confidentiality agreements with customers
• Update project status
• Organize experimental data and pictures, analyze the results and make a report

Project cycle

• It takes about 1~2 months.

Advantages

• Discuss with you the details of the expected interaction study, and develop experimental strategies / methods according to your requirements.
• The method of GFP as a reporter gene is highly sensitive, easy to detect and is a live test, which is not destructive to cells or tissues; Its expression in vivo is not restricted by biological types, genotypes, cell and tissue types; it does not require any substrates and exogenous cofactors to participate; it is convenient for early screening of genetically modified materials.
• The results of using Arabidopsis protoplasts as recipient cells are more reliable and convincing.
• The method of using tobacco mesophyll epidermal cells as recipient cells is cost-effective and has a short experimental period.
• Standardization system, scientific management.
• Leading technology platform conditions, first-class R&D and production equipment.
• Flexible quotation to meet your needs.

 

※ It should be noted that our service is only used for research.

Related Sections

• Plant Protein Interaction Services
• Plant Protein-Nucleic Acid Interaction Services
• Plant Promoter Research Services

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