Services
- Genetic Marker Assisted Breeding
- Plant Molecular Biology Research
- Research on DNA Level of Plant
- Research on Microspore Embryogenesis
- Plant Genetic Engineering
- Plant Genome Editing with CRISPR / Cas9
- Plant Genome Editing with TALEN
- Plant Genome Editing with ZFN
- RNAi Mediated Plant Gene Silencing
- Overexpression of Plant Genes
- Arabidopsis thaliana Transformation
- Oryza sativa Transformation
- Zea mays Transformation
- Triticum aestivum Transformation
- Medicago truncatula Transformation
- Glycine max Transformation
- Gossypium hirsutum Transformation
- Nicotiana tabacum Transformation
- Solanum lycopersicum Transformation
- Brassica napus Transformation
- Solanum tuberosum Transformation
- Virus-Induced Gene Silencing (VIGS) of Plant Genes
- Research on Protein Level of Plant
- Plant Epigenetic Modification Testing Services
- Sequencing-based Plant Breeding
- Plant DNA-level Sequencing Services
- Plant Genome De Novo Service
- Plant Whole Genome Resequencing Service
- Plant Reduced-Representation Genome Sequencing (RRGS)
- Plant Genetic Map Service
- BSA Trait Positioning of Plant
- Genome-Wide Association Study (GWAS) of Plant
- eQTL Analysis of Plant
- Plant Genetic Evolution Service
- Plant Pan-genome Sequencing
- Plant Whole Exome Sequencing Service
- Individual Selection Pressure Analysis of Plant
- Mixing-tank Selection Pressure Analysis of Plant
- Plant Whole Genome Survey
- Plant RNA Level Sequencing Services
- Eukaryotic Transcriptome Sequencing without Reference Genome
- Eukaryotic Transcriptome Sequencing with Reference Genome
- Prokaryotic Transcriptome Sequencing Analysis
- LncRNA Sequencing of Plant
- Plant Small RNA Sequencing
- Plant Circular RNA Sequencing
- Plant Comparative Transcriptome Service
- Plant Isoform-sequencing with Reference Genome
- Plant Isoform-sequencing without Reference Genome
- Ribo-seq of Plant
- Metatranscriptome Sequencing of Plant
- Plant Single Cell Level Sequencing Services
- Plant Epigenetics Level Sequencing Services
- Plant Proteomics Service
- Proteomics Qualitative Analysis in Plant
- Plant Protein Quantitative Analysis Service Based on Isotope Labeling (iTRAQ / TMT)
- Non-labeled Plant Protein Quantitative Analysis (Label-free / DIA)
- Plant Protein Targeted Quantitative Service (PRM / MRM / AQUA)
- Post-translational Modification Proteomics (PTMs) Service for Plant
- Plant Metabolomics Services
- Plant DNA-level Sequencing Services
- Other Services
- Plant CNV Analysis Service
- Plant Mutation Detection Service
- Plant Strain / Cell Level Services
- Plant Tissue and Cell Culture Services
- Plant Polyploidization Services
- Plant Haploidization Services
- Plant Phenotypic Analysis
- Plant Stress Response Indicators Analysis
- Plant Biochemical Analysis
- Plant Tissue and Cell Imaging Services
- Plant Disease Identification Services
- Plant Organelle Isolation Services
- Genetically Modified Plant Testing Services
- Seed Testing Services
Plant Mutation Detection Service
INQUIRYIntroductions
Mutations are genetic changes acquired in germ cells or non-germ (somatic) cells. Mutations can be expressed as insertions, deletions, or base pair changes in coding or non-coding regions, resulting in silent, missense or nonsense mutations. The process of selecting mutant plants with improved traits includes two main steps: mutation screening and mutation confirmation. Mutant screening involves the evaluation of a large number of mutant plants to identify rare mutant individuals that meet selection criteria (such as improved disease resistance, improved quality). Since the first domestication of plants, phenotypic screening has always been a common method for selecting improved crops in plant breeding. This process is time-consuming and greatly affected by environmental factors. Genotype screening (mutation confirmation) can be used to solve the bottlenecks and limitations of phenotypic evaluation. This process evaluates the plant's DNA to identify genetic variants that are related to the trait of interest. Once the DNA variation is determined, molecular detection methods can be developed to screen the population.
Services
With years of rich experience, Lifeasible provides customers with mutation analysis services, enabling scientists to quickly analyze and identify mutations that may affect the function of their target genes.
Analytical methods
- Transcriptome sequencing for extensive screening
- Digital PCR for targeted mutation analysis
Sample requirements
- Fresh tissue > 1g
- gDNA ≥ 50 μg
- Scope of test - complete coding exons, coding exons and introns of interest.
Project cycle
- The experiment cycle of the project is different depending on the selected method, please consult our technicians for details.
Feedback to customers
- Experimental procedures
- Original data file (.seq)
- Analysis results (nucleotide level mutation, amino acid changes), etc.
Service process
- Submit the relevant requirements and materials of the project.
- Discuss project details with our professional technical team.
- Revise the experimental plan based on the opinions after discussion.
- Both parties are satisfied and reach an agreement to sign a technical service contract.
- Carry out experiments and complete the content stipulated in the contract on time.
- After the project experiment is over, we will provide the original experimental data, analysis results, experimental procedures, experimental conditions, etc.
Advantages
- Lifeasible specializes in providing services related to agricultural molecular breeding, with standardized systems and scientific management.
- Advanced equipment, mature and stable methods.
- Optimized experimental procedures ensure extremely high efficiency and specificity.
- The one-stop service we provide makes your upstream and downstream experiments seamlessly connected, facilitates your experiments more conveniently and quickly.
- Personalized customized package service.
※ It should be noted that our service is only used for research.
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