Services
- Genetic Marker Assisted Breeding
- Plant Molecular Biology Research
- Research on DNA Level of Plant
- Research on Microspore Embryogenesis
- Plant Genetic Engineering
- Plant Genome Editing with CRISPR / Cas9
- Plant Genome Editing with TALEN
- Plant Genome Editing with ZFN
- RNAi Mediated Plant Gene Silencing
- Overexpression of Plant Genes
- Arabidopsis thaliana Transformation
- Oryza sativa Transformation
- Zea mays Transformation
- Triticum aestivum Transformation
- Medicago truncatula Transformation
- Glycine max Transformation
- Gossypium hirsutum Transformation
- Nicotiana tabacum Transformation
- Solanum lycopersicum Transformation
- Brassica napus Transformation
- Solanum tuberosum Transformation
- Virus-Induced Gene Silencing (VIGS) of Plant Genes
- Research on Protein Level of Plant
- Plant Epigenetic Modification Testing Services
- Sequencing-based Plant Breeding
- Plant DNA-level Sequencing Services
- Plant Genome De Novo Service
- Plant Whole Genome Resequencing Service
- Plant Reduced-Representation Genome Sequencing (RRGS)
- Plant Genetic Map Service
- BSA Trait Positioning of Plant
- Genome-Wide Association Study (GWAS) of Plant
- eQTL Analysis of Plant
- Plant Genetic Evolution Service
- Plant Pan-genome Sequencing
- Plant Whole Exome Sequencing Service
- Individual Selection Pressure Analysis of Plant
- Mixing-tank Selection Pressure Analysis of Plant
- Plant Whole Genome Survey
- Plant RNA Level Sequencing Services
- Eukaryotic Transcriptome Sequencing without Reference Genome
- Eukaryotic Transcriptome Sequencing with Reference Genome
- Prokaryotic Transcriptome Sequencing Analysis
- LncRNA Sequencing of Plant
- Plant Small RNA Sequencing
- Plant Circular RNA Sequencing
- Plant Comparative Transcriptome Service
- Plant Isoform-sequencing with Reference Genome
- Plant Isoform-sequencing without Reference Genome
- Ribo-seq of Plant
- Metatranscriptome Sequencing of Plant
- Plant Single Cell Level Sequencing Services
- Plant Epigenetics Level Sequencing Services
- Plant Proteomics Service
- Proteomics Qualitative Analysis in Plant
- Plant Protein Quantitative Analysis Service Based on Isotope Labeling (iTRAQ / TMT)
- Non-labeled Plant Protein Quantitative Analysis (Label-free / DIA)
- Plant Protein Targeted Quantitative Service (PRM / MRM / AQUA)
- Post-translational Modification Proteomics (PTMs) Service for Plant
- Plant Metabolomics Services
- Plant DNA-level Sequencing Services
- Other Services
- Plant CNV Analysis Service
- Plant Mutation Detection Service
- Plant Strain / Cell Level Services
- Plant Tissue and Cell Culture Services
- Plant Polyploidization Services
- Plant Haploidization Services
- Plant Phenotypic Analysis
- Plant Stress Response Indicators Analysis
- Plant Biochemical Analysis
- Plant Tissue and Cell Imaging Services
- Plant Disease Identification Services
- Plant Organelle Isolation Services
- Genetically Modified Plant Testing Services
- Seed Testing Services
Plant DNA Recombination
INQUIRYIntroductions
Plant DNA recombination is a technology that recombines and expresses plant DNA in vitro. It regards plant DNA as a component, connects it with the vector DNA (another component), and then transfers the recombinant into the host cell so that the plant gene in the host bacterial cell can be expressed. The introduced plant gene multiplies (cloning) as the host cell multiplies. Plant DNA recombination technology is widely used in agricultural molecular breeding, such as improving the insect resistance, virus resistance, herbicide resistance and stress resistance, improving seed storage protein, changing fruit maturity, improving flower decoration quality, and improving cytoplasm male sterility and self-incompatibility and other molecular researches.
Services
Lifeasible has established a complete plant DNA recombination platform to help you carry out relevant experimental services.
Workflow of plant DNA recombination
- Separate
- Digestion
- Connection
- Transform
- Screen
Separate vectors and plant genes separately
Restriction endonuclease
Connect the vector and plant gene to form a recombinant
Transfer the recombinant into the host cell
Screening and identification of plant genes
Steps for recombinant DNA technology (Deans T L, 2014)
Service requirements
- If you provide tissue or section samples, please indicate the species and source of the sample.
- If you provide the primer yourself, please indicate the specific OD number if it is dry, and indicate the specific concentration if it is in a liquid state.
- If you provide PCR products, please indicate the concentration, and preferably above 5μg (with electrophoresis photos, and mark Marker).
- If plasmid is provided, it is better to be above 4μg.
- If you provide a vector, please indicate the restriction site used for cloning. If it is a commercial vector, please provide the manufacturer and standard name; if it is constructed for you, please indicate the multiple cloning sites, resistance, molecular weight and other information.
- If glycerol bacteria or puncture bacteria are provided, please indicate antibiotics and ensure high activity.
Provided by Lifeasible
- Raw data and pictures of the experiment
- Lyophilized powder of plasmid and puncture bacteria
- Experiment report (experimental equipment, reagents, methods, results, conclusions)
Advantages
- Quality assurance, efficient service
- Standardization system, scientific management
- Complete layout of the biotechnology chain, seamless connection with downstream experiments
- Leading technology platform conditions, first-class R&D and production equipment
Reference
- Deans T L. Parallel Networks: Synthetic Biology and Artificial Intelligence[J]. ACM Journal on Emerging Technologies in Computing Systems (JETC) - Special Issue on Computational Synthetic Biology and Regular Papers, 2014.
※ It should be noted that our service is only used for research.
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