Plant KASP Analysis Service

INQUIRY

Introductions

SNP genotyping requires the detection of a small number of SNPs in a large number of samples, while gene chip or NGS technology is not suitable for this stage of research due to its high cost. KASP (Kompetitive Allele Specific PCR) is one of the mainstream SNP detection methods in the world due to its economical and flexible characteristics. It replaces traditional high-throughput sequencing and plays an important role in SNP typing research. It can make accurate biallelic judgments on SNPs and InDels at specific sites in a wide range of genomic DNA samples (even some complex genomic DNA samples). This technology uses universal fluorescent probes to achieve SNP typing by a simple touch-down PCR method. KASP helps to screen out genes that control favorable traits of plants, guides subsequent variety improvement, and also facilitates genetic verification of improved varieties.

Services

With years of rich experience, Lifeasible uses KASP as its core technology to provide flexible and applicable genotyping solutions for genotyping analysis, KASP Assay development, SNP verification after NGS, and agricultural population research.

Testing platform

• LGC SNP genotyping technology platform
• Douglas array tape platform

Sample requirements

• Number of samples ≥ 24 (including 2 blank spots)
• Sample concentration = 200 ng / μl ~ 1000 ng / μl
• Sample ≥ 100 μl (the number of detection marks is more than 192 or less than 16, according to the specific inspection content)
• No obvious impurities, macromolecules and color, OD260 / 230 ＞1

Project cycle

• A standard experimental procedure takes about 10-15 working days.

Feedback to customers

• Fluorescence color and intensity in each microwell on the entire PCR plate
• Three cluster distribution map of all microwell results
• Excel table of fluorescence value of each well
• The typing results of each sample obtained by importing the data in the excel table into the software
• Other relevant information required by the customer

Service process

• Submit the relevant requirements and materials of the project.
• Discuss project details with our professional technical team.
• Revise the experimental plan based on the opinions after discussion.
• Both parties are satisfied and reach an agreement to sign a technical service contract.
• Carry out experiments and complete the content stipulated in the contract on time.
• After the project experiment is over, we will provide the original experimental data, analysis results, experimental procedures, experimental conditions, etc.

Advantages

• Lifeasible specializes in providing services related to agricultural molecular breeding, with standardized systems and scientific management.
• Advanced equipment, mature and stable methods.
• Optimized experimental procedures ensure extremely high efficiency and specificity.
• The one-stop service we provide makes your upstream and downstream experiments seamlessly connected, facilitates your experiments more conveniently and quickly.
• Personalized customized package service.

 

※ It should be noted that our service is only used for research.

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