Services
- Genetic Marker Assisted Breeding
- Plant Molecular Biology Research
- Research on DNA Level of Plant
- Research on Microspore Embryogenesis
- Plant Genetic Engineering
- Plant Genome Editing with CRISPR / Cas9
- Plant Genome Editing with TALEN
- Plant Genome Editing with ZFN
- RNAi Mediated Plant Gene Silencing
- Overexpression of Plant Genes
- Arabidopsis thaliana Transformation
- Oryza sativa Transformation
- Zea mays Transformation
- Triticum aestivum Transformation
- Medicago truncatula Transformation
- Glycine max Transformation
- Gossypium hirsutum Transformation
- Nicotiana tabacum Transformation
- Solanum lycopersicum Transformation
- Brassica napus Transformation
- Solanum tuberosum Transformation
- Virus-Induced Gene Silencing (VIGS) of Plant Genes
- Research on Protein Level of Plant
- Plant Epigenetic Modification Testing Services
- Sequencing-based Plant Breeding
- Plant DNA-level Sequencing Services
- Plant Genome De Novo Service
- Plant Whole Genome Resequencing Service
- Plant Reduced-Representation Genome Sequencing (RRGS)
- Plant Genetic Map Service
- BSA Trait Positioning of Plant
- Genome-Wide Association Study (GWAS) of Plant
- eQTL Analysis of Plant
- Plant Genetic Evolution Service
- Plant Pan-genome Sequencing
- Plant Whole Exome Sequencing Service
- Individual Selection Pressure Analysis of Plant
- Mixing-tank Selection Pressure Analysis of Plant
- Plant Whole Genome Survey
- Plant RNA Level Sequencing Services
- Eukaryotic Transcriptome Sequencing without Reference Genome
- Eukaryotic Transcriptome Sequencing with Reference Genome
- Prokaryotic Transcriptome Sequencing Analysis
- LncRNA Sequencing of Plant
- Plant Small RNA Sequencing
- Plant Circular RNA Sequencing
- Plant Comparative Transcriptome Service
- Plant Isoform-sequencing with Reference Genome
- Plant Isoform-sequencing without Reference Genome
- Ribo-seq of Plant
- Metatranscriptome Sequencing of Plant
- Plant Single Cell Level Sequencing Services
- Plant Epigenetics Level Sequencing Services
- Plant Proteomics Service
- Proteomics Qualitative Analysis in Plant
- Plant Protein Quantitative Analysis Service Based on Isotope Labeling (iTRAQ / TMT)
- Non-labeled Plant Protein Quantitative Analysis (Label-free / DIA)
- Plant Protein Targeted Quantitative Service (PRM / MRM / AQUA)
- Post-translational Modification Proteomics (PTMs) Service for Plant
- Plant Metabolomics Services
- Plant DNA-level Sequencing Services
- Other Services
- Plant CNV Analysis Service
- Plant Mutation Detection Service
- Plant Strain / Cell Level Services
- Plant Tissue and Cell Culture Services
- Plant Polyploidization Services
- Plant Haploidization Services
- Plant Phenotypic Analysis
- Plant Stress Response Indicators Analysis
- Plant Biochemical Analysis
- Plant Tissue and Cell Imaging Services
- Plant Disease Identification Services
- Plant Organelle Isolation Services
- Genetically Modified Plant Testing Services
- Seed Testing Services
Plant KASP Analysis Service
INQUIRYIntroductions
SNP genotyping requires the detection of a small number of SNPs in a large number of samples, while gene chip or NGS technology is not suitable for this stage of research due to its high cost. KASP (Kompetitive Allele Specific PCR) is one of the mainstream SNP detection methods in the world due to its economical and flexible characteristics. It replaces traditional high-throughput sequencing and plays an important role in SNP typing research. It can make accurate biallelic judgments on SNPs and InDels at specific sites in a wide range of genomic DNA samples (even some complex genomic DNA samples). This technology uses universal fluorescent probes to achieve SNP typing by a simple touch-down PCR method. KASP helps to screen out genes that control favorable traits of plants, guides subsequent variety improvement, and also facilitates genetic verification of improved varieties.
Services
With years of rich experience, Lifeasible uses KASP as its core technology to provide flexible and applicable genotyping solutions for genotyping analysis, KASP Assay development, SNP verification after NGS, and agricultural population research.
Testing platform
- LGC SNP genotyping technology platform
- Douglas array tape platform
Sample requirements
- Number of samples ≥ 24 (including 2 blank spots)
- Sample concentration = 200 ng / μl ~ 1000 ng / μl
- Sample ≥ 100 μl (the number of detection marks is more than 192 or less than 16, according to the specific inspection content)
- No obvious impurities, macromolecules and color, OD260 / 230 ＞1
Project cycle
- A standard experimental procedure takes about 10-15 working days.
Feedback to customers
- Fluorescence color and intensity in each microwell on the entire PCR plate
- Three cluster distribution map of all microwell results
- Excel table of fluorescence value of each well
- The typing results of each sample obtained by importing the data in the excel table into the software
- Other relevant information required by the customer
Service process
- Submit the relevant requirements and materials of the project.
- Discuss project details with our professional technical team.
- Revise the experimental plan based on the opinions after discussion.
- Both parties are satisfied and reach an agreement to sign a technical service contract.
- Carry out experiments and complete the content stipulated in the contract on time.
- After the project experiment is over, we will provide the original experimental data, analysis results, experimental procedures, experimental conditions, etc.
Advantages
- Lifeasible specializes in providing services related to agricultural molecular breeding, with standardized systems and scientific management.
- Advanced equipment, mature and stable methods.
- Optimized experimental procedures ensure extremely high efficiency and specificity.
- The one-stop service we provide makes your upstream and downstream experiments seamlessly connected, facilitates your experiments more conveniently and quickly.
- Personalized customized package service.
※ For research or industrial use.
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