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- Chestnut Genetic Modification Service
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Chestnut Resistance Breeding
INQUIRYAs an important economic forest species, chestnut has high edible, medicinal, and industrial value. However, chestnuts face various adversity stresses during the growth process, and these adversities seriously affect the yield, quality, and economic benefits of chestnuts. Traditional chestnut resistance breeding can hardly meet the needs of the chestnut industry, and the use of advanced biotechnology can bring new solutions for chestnut resistance breeding. Lifeasible is committed to providing advanced technical support and effective solutions for chestnut resistance molecular breeding, and our advanced technological platform can greatly help promote the progress of chestnut resistance breeding research.
Explore Our Services of Chestnut Resistance Breeding
Chestnut heat resistance service
High temperatures are harmful to chestnut, including affecting bud differentiation, high temperatures during flowering (e.g., over 27°C) will inhibit pollen germination and fertilization, reducing the rate of fruit set, and high temperatures above 35°C during the grouting period will affect the development of fruits, leading to empty bracts or shriveling.
We provide technical support for the following services.
- Mining of genes related to heat resistance. Through high-throughput sequencing technology and bioinformatics analysis, we conduct a comprehensive scan of the chestnut genome to dig out genes closely associated with heat resistance and provide key genetic resources for heat resistance breeding.
- Gene function verification. Using gene editing technology to verify the function of the tapped heat resistance genes, we will observe the physiological and biochemical indexes and heat resistance performance of chestnut plants under high temperature conditions by knocking out or overexpressing these genes, to clarify their specific roles in heat resistance of chestnut.
- Development of molecular markers for heat resistance. The development of specific molecular markers based on heat resistance-related genes can be used for early screening of seedlings or non-flowering plants during chestnut breeding to rapidly and accurately identify individuals with heat resistance potential, improve breeding efficiency, and shorten the breeding cycle.
Chestnut cold resistance service
The effects of low temperatures on the growth and development of chestnuts include delayed budding and impeded pollination during flowering, resulting in reduced yields due to empty bracts, delayed fruit ripening, and reduced sugar content and quality.
We provide the following services.
- Cloning and expression analysis of genes related to cold resistance. By cloning the genes related to cold resistance in chestnut, we can help researchers to study the expression pattern and change rule of these genes under different low-temperature conditions, reveal the molecular mechanism of cold resistance in chestnut, and provide theoretical support for breeding cold resistance.
- Genetic improvement of cold resistance. We introduced exogenous cold resistance genes into chestnut embryos or healing tissues through genetic engineering to breed transgenic chestnut plants with stronger cold resistance.
Chestnut drought resistance service
We also provide technical support for chestnut drought resistance research, including the use of multi-omics technology, in-depth study of the molecular response mechanism of chestnut under drought stress, and advanced biotechnology to breed chestnut varieties with excellent drought resistance.
Our Advanced Technology Platform
| In vitro culture and gene editing technology platform | We have established a tissue culture technology platform that can help researchers break through the challenges of aseptic culture of axillary buds of adult trees to promote the rapid propagation of good seeds. We further provide a platform for gene editing breeding by optimizing the healing tissue transient transformation system. |
| Multi-omics technology platform | We have established a platform for sequencing the chestnut genome and have also revealed the expression patterns of genes related to metabolism and signaling in bud development by sequencing the transcriptome of chestnut new shoots, which provides a basis for targeted breeding. |
| Efficient screening technology platform | We have realized precise identification of resistance phenotypes through pathogen inoculation combined with spot analysis. In addition, molecular detection technology was utilized for rapid phytoplasma detection to enhance the efficiency of disease resistance evaluation. |
Why Choose Us?
- Professional research team. Our expert team is familiar with the biological characteristics of chestnut and resistance breeding technology, and can provide customers with all-around, high-quality technical services and solutions.
- Advanced technology platform. We constantly optimize and update our technical system and equipment to ensure we can provide high-quality molecular breeding services in chestnut resistance for our customers.
- Complete biological services. We focus on communication and exchange with customers, timely understanding of customer needs, and aim to provide customers with perfect after-sales service.
With its advanced technology platform, rich experience in chestnut disease resistance breeding, and high-quality biotechnology services, Lifeasible is committed to promoting the research and development of chestnut resistance breeding. If you are interested in us, please feel free to contact us.
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