Services
- Genetic Marker Assisted Breeding
- Plant Molecular Biology Research
- Research on DNA Level of Plant
- Research on Microspore Embryogenesis
- Plant Genetic Engineering
- Plant Genome Editing with CRISPR / Cas9
- Plant Genome Editing with TALEN
- Plant Genome Editing with ZFN
- RNAi Mediated Plant Gene Silencing
- Overexpression of Plant Genes
- Arabidopsis thaliana Transformation
- Oryza sativa Transformation
- Zea mays Transformation
- Triticum aestivum Transformation
- Medicago truncatula Transformation
- Glycine max Transformation
- Gossypium hirsutum Transformation
- Nicotiana tabacum Transformation
- Solanum lycopersicum Transformation
- Brassica napus Transformation
- Solanum tuberosum Transformation
- Virus-Induced Gene Silencing (VIGS) of Plant Genes
- Research on Protein Level of Plant
- Plant Epigenetic Modification Testing Services
- Sequencing-based Plant Breeding
- Plant DNA-level Sequencing Services
- Plant Genome De Novo Service
- Plant Whole Genome Resequencing Service
- Plant Reduced-Representation Genome Sequencing (RRGS)
- Plant Genetic Map Service
- BSA Trait Positioning of Plant
- Genome-Wide Association Study (GWAS) of Plant
- eQTL Analysis of Plant
- Plant Genetic Evolution Service
- Plant Pan-genome Sequencing
- Plant Whole Exome Sequencing Service
- Individual Selection Pressure Analysis of Plant
- Mixing-tank Selection Pressure Analysis of Plant
- Plant Whole Genome Survey
- Plant RNA Level Sequencing Services
- Eukaryotic Transcriptome Sequencing without Reference Genome
- Eukaryotic Transcriptome Sequencing with Reference Genome
- Prokaryotic Transcriptome Sequencing Analysis
- LncRNA Sequencing of Plant
- Plant Small RNA Sequencing
- Plant Circular RNA Sequencing
- Plant Comparative Transcriptome Service
- Plant Isoform-sequencing with Reference Genome
- Plant Isoform-sequencing without Reference Genome
- Ribo-seq of Plant
- Metatranscriptome Sequencing of Plant
- Plant Single Cell Level Sequencing Services
- Plant Epigenetics Level Sequencing Services
- Plant Proteomics Service
- Proteomics Qualitative Analysis in Plant
- Plant Protein Quantitative Analysis Service Based on Isotope Labeling (iTRAQ / TMT)
- Non-labeled Plant Protein Quantitative Analysis (Label-free / DIA)
- Plant Protein Targeted Quantitative Service (PRM / MRM / AQUA)
- Post-translational Modification Proteomics (PTMs) Service for Plant
- Plant Metabolomics Services
- Plant DNA-level Sequencing Services
- Other Services
- Plant CNV Analysis Service
- Plant Mutation Detection Service
- Plant Strain / Cell Level Services
- Plant Tissue and Cell Culture Services
- Plant Polyploidization Services
- Plant Haploidization Services
- Plant Phenotypic Analysis
- Plant Stress Response Indicators Analysis
- Plant Biochemical Analysis
- Plant Tissue and Cell Imaging Services
- Plant Disease Identification Services
- Plant Organelle Isolation Services
- Genetically Modified Plant Testing Services
- Seed Testing Services
Solanum tuberosum Transformation
INQUIRYIntroductions
Potato, whose scientific name is Solanum tuberosum L., belongs to the annual herbaceous plant of the class Dicotyledon, Solanaceae, and Solanum. It is an economic crop with high yield, strong adaptability, wide distribution, rich nutrition and high economic value, which is suitable for grain, vegetable, feed, and industrial raw material. Potato is vulnerable to a variety of viruses during the growth period, causing germplasm degradation and yield reduction. The development of potato virus-resistant genetic engineering has alleviated this hazard. The improvement of potato quality through genetic engineering has the advantages of clear genes, known products, specific targets, accuracy and rapidity and strong predictability. In addition, the potato reproduces asexually, and the asexual offspring of the transgenic plants do not separate, which brings much convenience to the detection and identification of the transgenic plants.
Services
Lifeasible has established a potato genetic transformation platform. We use Agrobacterium-mediated method or gene gun method to realize potato genetic transformation. Customers can provide target genes, plasmids, Agrobacterium, etc., and we will complete the entire experimental process from constructing vectors to obtaining transgenic vaccines. We can use mature potato varieties to provide large-scale and standardized transgenic technology services, and we can also undertake R&D outsourcing of transformation systems for designated varieties.
Customer provided materials
- The sequence information or Gene Bank ID of target gene
- Constructed E. coli plasmid vector, but provides clear drug resistance information for screening.
- Constructed transformation vectors, or entrust our company to construct various vectors, such as gene cloning and expression, silencing, knockout (CRISPR/Cas9), VIGS, etc.
- Unconventional receptor material
Our company can undertake gene amplification and vector construction services, which are charged separately.
※ We do not accept the transformation service of lethal genes or severely defective genes. If there are any of the above, please inform our company in advance.
Solanum tuberosum transformation methods
- Agrobacterium-mediated transformation
- Gene gun bombardment method
Receptor material
- Recipient species
- Recipient types
Huaen1 and other species
Test tube potato
Workflow of potato transformation by Agrobacterium-mediated method
- Vector construction
- Potato receptor material cultivation
- Agrobacterium-mediated genetic transformation and screening
- Strong plantlets and rootage of regenerated green seedlings
- PCR detection of foreign genes in T0 generation transgenic seedlings
Feedback to customers
- Complete experimental methods, original experimental records, instrument parameters, reagents and consumables information.
- Pictures of explant infection, directing callus, embryogenic callus, tissue differentiation, plant regeneration, etc.
- T0 generation transgenic vaccine: If we use the vector and recipient provided by us, there will be 8-10 T0 generation positive vaccines for each transformation experiment; if customers need to use other vectors and recipients, we usually only promise to get T0 generation positive vaccines 6-8 strains.
Service process
Project cycle
- It takes about 4~6 months.
Advantages
- Agrobacterium-mediated transformation is fast, efficient, and stable, and mainly single-copy insertion.
- For commonly used potato varieties, sufficient sterile seedlings are prepared all year round, and transformation experiments can be carried out at any time to save customers' time.
- We have a variety of labeled vectors and media to provide you with a wide range of choices.
- The technical team engaged in plant genetic transformation for many years guarantees the success rate of the experiment.
- Complete layout of the biotechnology chain, seamless connection with downstream experiments.
- Leading technology platform conditions, first-class R&D and production equipment.
- Personalized customized package service.
※ It should be noted that our service is only used for research.
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