Services
- Genetic Marker Assisted Breeding
- Plant Molecular Biology Research
- Research on DNA Level of Plant
- Research on Microspore Embryogenesis
- Plant Genetic Engineering
- Plant Genome Editing with CRISPR / Cas9
- Plant Genome Editing with TALEN
- Plant Genome Editing with ZFN
- RNAi Mediated Plant Gene Silencing
- Overexpression of Plant Genes
- Arabidopsis thaliana Transformation
- Oryza sativa Transformation
- Zea mays Transformation
- Triticum aestivum Transformation
- Medicago truncatula Transformation
- Glycine max Transformation
- Gossypium hirsutum Transformation
- Nicotiana tabacum Transformation
- Solanum lycopersicum Transformation
- Brassica napus Transformation
- Solanum tuberosum Transformation
- Virus-Induced Gene Silencing (VIGS) of Plant Genes
- Research on Protein Level of Plant
- Plant Epigenetic Modification Testing Services
- Sequencing-based Plant Breeding
- Plant DNA-level Sequencing Services
- Plant Genome De Novo Service
- Plant Whole Genome Resequencing Service
- Plant Reduced-Representation Genome Sequencing (RRGS)
- Plant Genetic Map Service
- BSA Trait Positioning of Plant
- Genome-Wide Association Study (GWAS) of Plant
- eQTL Analysis of Plant
- Plant Genetic Evolution Service
- Plant Pan-genome Sequencing
- Plant Whole Exome Sequencing Service
- Individual Selection Pressure Analysis of Plant
- Mixing-tank Selection Pressure Analysis of Plant
- Plant Whole Genome Survey
- Plant RNA Level Sequencing Services
- Eukaryotic Transcriptome Sequencing without Reference Genome
- Eukaryotic Transcriptome Sequencing with Reference Genome
- Prokaryotic Transcriptome Sequencing Analysis
- LncRNA Sequencing of Plant
- Plant Small RNA Sequencing
- Plant Circular RNA Sequencing
- Plant Comparative Transcriptome Service
- Plant Isoform-sequencing with Reference Genome
- Plant Isoform-sequencing without Reference Genome
- Ribo-seq of Plant
- Metatranscriptome Sequencing of Plant
- Plant Single Cell Level Sequencing Services
- Plant Epigenetics Level Sequencing Services
- Plant Proteomics Service
- Proteomics Qualitative Analysis in Plant
- Plant Protein Quantitative Analysis Service Based on Isotope Labeling (iTRAQ / TMT)
- Non-labeled Plant Protein Quantitative Analysis (Label-free / DIA)
- Plant Protein Targeted Quantitative Service (PRM / MRM / AQUA)
- Post-translational Modification Proteomics (PTMs) Service for Plant
- Plant Metabolomics Services
- Plant DNA-level Sequencing Services
- Other Services
- Plant CNV Analysis Service
- Plant Mutation Detection Service
- Plant Strain / Cell Level Services
- Plant Tissue and Cell Culture Services
- Plant Polyploidization Services
- Plant Haploidization Services
- Plant Phenotypic Analysis
- Plant Stress Response Indicators Analysis
- Plant Biochemical Analysis
- Plant Tissue and Cell Imaging Services
- Plant Disease Identification Services
- Plant Organelle Isolation Services
- Genetically Modified Plant Testing Services
- Seed Testing Services
Glycine max Transformation
INQUIRYIntroductions
Soybean is an annual herb of the leguminous soybean genus, and its scientific name is Glycine max (Linn.) Merr. It is an important oil and food crop. Its seeds contain about 40% protein, 20% fat, and are also rich in 8 essential amino acids required by the human body. It is the most ideal source of plant protein for humans. It also plays an indispensable role in industrial raw materials, food crystal processing and agricultural production. Due to obstacles such as hybrid incompatibility and gene chain effects between different species, traditional Breeding will prevent the combination of different excellent genes from reaching the optimal level. As the development process of genetically modified technology is accelerating, the integration of foreign genes into the soybean genome to create new genetically modified soybean materials has become an important means of soybean Molecular Breeding.
Services
At present, the commonly used methods of soybean genetic transformation include Agrobacterium-mediated method, gene gun method, pollen tube channel method, polyethylene glycol method, electric shock transformation method, ultrasonic assisted transformation method and vacuum filtration assisted treatment method. Lifeasible has established a soybean genetic transformation platform to provide Agrobacterium-mediated and particle bombardment transformation systems for researchers who use soybeans as their research objects. We have an experienced team that can help you solve technical problems and help your project succeed.
Customer provided materials
- If only gene sequence information (or Gene Bank ID) is provided, our company can undertake gene amplification and vector construction services, which are charged separately.
- Construct a vector of Escherichia coli, plasmid or Agrobacterium, but provide clear resistance information for screening.
- Unconventional receptor material
※ We do not accept the transformation service of lethal genes or severely defective genes. If there are any of the above, please inform our company in advance.
Glycine max genetic transformation methods
- Microprojectile bombardment transformation method.
- Agrobacterium-mediated transformation method.
Receptor material
- Recipient species
- Recipient types
Wiliam82, Tianlong1
For microprojectile bombardment, the explants of shoot meristemsand / embryogenic suspension cultures are commonly used.
For Agrobacterium-mediated transformation, wounded axillary meristem located in cotyledonary nodes are used (as it allows rapid reproduction via direct shoot organogenesis, bypassing the callus formation step).
Workflow of Glycine max transformation
Feedback to customers
- Complete experimental methods, original experimental records, instrument parameters, reagents and consumables information.
- Vector construction sequencing report and pictures.
- Pictures of explant infection, induced callus, embryogenic callus, tissue differentiation, plant regeneration, etc.
- Gel electrophoresis diagram of PCR detection of target genes in T0 generation regenerated plants.
- T0 generation transgenic vaccine: If we use the vector and recipient provided by us, the T0 generation positive vaccine for each transformation experiment is ≥8; if the customer needs to use other vectors and recipients, we usually only promise to get the T0 generation positive vaccine 6-8 strains.
Service process
Project cycle
- It takes about 7-9 months from the construction of the vector to the test results of the T0 generation transgenic seedlings. And experiments can be carried out throughout the year.
Advantages
- We have a variety of labeled vectors and media to provide you with a wide range of choices.
- The Agrobacterium-mediated method has good stability, high fault tolerance and high transformation efficiency.
- The genotype of the particle bombardment method is not limited, and this method can transfer multiple copies of recombinant DNA fragments at the same time.
- Standardization system, scientific management.
- Personalized customized package service.
- Perfect service support. Provide professional technical consultation and guidance in the early stage to optimize the project; communicate at any time during the experiment to keep abreast of the progress; after the project is completed, provide complete results according to the contract.
※ For research or industrial use.
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