Services
- Genetic Marker Assisted Breeding
- Plant Molecular Biology Research
- Research on DNA Level of Plant
- Research on Microspore Embryogenesis
- Plant Genetic Engineering
- Plant Genome Editing with CRISPR / Cas9
- Plant Genome Editing with TALEN
- Plant Genome Editing with ZFN
- RNAi Mediated Plant Gene Silencing
- Overexpression of Plant Genes
- Arabidopsis thaliana Transformation
- Oryza sativa Transformation
- Zea mays Transformation
- Triticum aestivum Transformation
- Medicago truncatula Transformation
- Glycine max Transformation
- Gossypium hirsutum Transformation
- Nicotiana tabacum Transformation
- Solanum lycopersicum Transformation
- Brassica napus Transformation
- Solanum tuberosum Transformation
- Virus-Induced Gene Silencing (VIGS) of Plant Genes
- Research on Protein Level of Plant
- Plant Epigenetic Modification Testing Services
- Sequencing-based Plant Breeding
- Plant DNA-level Sequencing Services
- Plant Genome De Novo Service
- Plant Whole Genome Resequencing Service
- Plant Reduced-Representation Genome Sequencing (RRGS)
- Plant Genetic Map Service
- BSA Trait Positioning of Plant
- Genome-Wide Association Study (GWAS) of Plant
- eQTL Analysis of Plant
- Plant Genetic Evolution Service
- Plant Pan-genome Sequencing
- Plant Whole Exome Sequencing Service
- Individual Selection Pressure Analysis of Plant
- Mixing-tank Selection Pressure Analysis of Plant
- Plant Whole Genome Survey
- Plant RNA Level Sequencing Services
- Eukaryotic Transcriptome Sequencing without Reference Genome
- Eukaryotic Transcriptome Sequencing with Reference Genome
- Prokaryotic Transcriptome Sequencing Analysis
- LncRNA Sequencing of Plant
- Plant Small RNA Sequencing
- Plant Circular RNA Sequencing
- Plant Comparative Transcriptome Service
- Plant Isoform-sequencing with Reference Genome
- Plant Isoform-sequencing without Reference Genome
- Ribo-seq of Plant
- Metatranscriptome Sequencing of Plant
- Plant Single Cell Level Sequencing Services
- Plant Epigenetics Level Sequencing Services
- Plant Proteomics Service
- Proteomics Qualitative Analysis in Plant
- Plant Protein Quantitative Analysis Service Based on Isotope Labeling (iTRAQ / TMT)
- Non-labeled Plant Protein Quantitative Analysis (Label-free / DIA)
- Plant Protein Targeted Quantitative Service (PRM / MRM / AQUA)
- Post-translational Modification Proteomics (PTMs) Service for Plant
- Plant Metabolomics Services
- Plant DNA-level Sequencing Services
- Other Services
- Plant CNV Analysis Service
- Plant Mutation Detection Service
- Plant Strain / Cell Level Services
- Plant Tissue and Cell Culture Services
- Plant Polyploidization Services
- Plant Haploidization Services
- Plant Phenotypic Analysis
- Plant Stress Response Indicators Analysis
- Plant Biochemical Analysis
- Plant Tissue and Cell Imaging Services
- Plant Disease Identification Services
- Plant Organelle Isolation Services
- Genetically Modified Plant Testing Services
- Seed Testing Services
Plant Reduced-Representation Genome Sequencing (RRGS)
INQUIRYINTRODUCTIONS
Population genetics is a discipline that studies the genetic structure of a population and its changing laws. Its research objects are natural populations, generally different populations of a certain species (such as different species, different regions, etc.). The research of population genetics based on simplified genome sequencing is to develop a large number of SNP markers from the whole genome, and then carry out the genomics hotspot analysis content such as population genetic evolution, phylogeny, germplasm resource identification, Molecular marker development, DNA fingerprinting construction and so on. At present, plant reduced-representation genome sequencing technology (RRGS) has been widely used in population analysis. It is not limited by the genome, can reduce the complexity of the genome, has a low amount of data, is simple to operate, and saves costs. It is especially suitable for large sample size research.
SERVICES
With years of rich experience, Lifeasible provides plant reduced-representation genome sequencing service. We provide a one-stop service model. We complete a series of processes from sample extraction, quality testing, sequencing, biological information analysis to making reports. The analysis content includes standard analysis and advanced analysis or customized analysis.
Workflow of plant reduced-representation genome sequencing service
Analysis content
| Reference genome | No reference genome |
|---|---|
| Genomic electronic digestion assessment Electronic digestion assessment statistics Genomic chromosome tags statistics Chromosome distribution of restriction sites |
Sequencing quality assessment |
| Sequencing data evaluation | |
| Raw data filtering | Raw data filtering |
| Comparison of data statistics | GBS tag clustering / RAD tag clustering and splicing |
| Variant analysis SNP detection, annotation and statistics InDel detection, annotation and statistics |
Variant analysis SNP detection, annotation and statistics InDel detection, annotation and statistics |
| Group structure analysis Population principal component analysis (PCA analysis) Population evolutionary tree analysis Group structure analysis (Structure graph analysis) |
Group structure analysis Group principal component analysis (PCA analysis) Population evolutionary tree analysis Group structure analysis (Structure graph analysis) |
Platform
Illumina HiSeq 2000
Sample requirements
- Sample concentration≥25ng/μl, total amount≥2μg, OD260/280＝1.8~2.0.
(The amount of DNA required is related to the number of libraries constructed and the type of library)
Project cycle
- The entire project generally takes about 2 months, and the specific completion time depends on the size and complexity of the genome. The time is calculated from the time the sample is qualified, and does not include the time when the experiment is stalled due to sample problems.
Feedback to customers
- Raw data
- A project report includes experimental process, instrument parameters, analysis methods and results, etc.
Service process
ADVANTAGES
- Our experienced researchers help you with scientific plan design.
- Our method is excellent, the cycle is short, and the accuracy is high.
- Excellent analysis creates project results.
- We combine rich project experience, professional project planning guidance and analysis process to ensure rapid and accurate assessment of genome characteristics.
- Personalized customized package service.
※ It should be noted that our service is only used for research.
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