Services
- Genetic Marker Assisted Breeding
- Plant Molecular Biology Research
- Research on DNA Level of Plant
- Research on Microspore Embryogenesis
- Plant Genetic Engineering
- Plant Genome Editing with CRISPR / Cas9
- Plant Genome Editing with TALEN
- Plant Genome Editing with ZFN
- RNAi Mediated Plant Gene Silencing
- Overexpression of Plant Genes
- Arabidopsis thaliana Transformation
- Oryza sativa Transformation
- Zea mays Transformation
- Triticum aestivum Transformation
- Medicago truncatula Transformation
- Glycine max Transformation
- Gossypium hirsutum Transformation
- Nicotiana tabacum Transformation
- Solanum lycopersicum Transformation
- Brassica napus Transformation
- Solanum tuberosum Transformation
- Virus-Induced Gene Silencing (VIGS) of Plant Genes
- Research on Protein Level of Plant
- Plant Epigenetic Modification Testing Services
- Sequencing-based Plant Breeding
- Plant DNA-level Sequencing Services
- Plant Genome De Novo Service
- Plant Whole Genome Resequencing Service
- Plant Reduced-Representation Genome Sequencing (RRGS)
- Plant Genetic Map Service
- BSA Trait Positioning of Plant
- Genome-Wide Association Study (GWAS) of Plant
- eQTL Analysis of Plant
- Plant Genetic Evolution Service
- Plant Pan-genome Sequencing
- Plant Whole Exome Sequencing Service
- Individual Selection Pressure Analysis of Plant
- Mixing-tank Selection Pressure Analysis of Plant
- Plant Whole Genome Survey
- Plant RNA Level Sequencing Services
- Eukaryotic Transcriptome Sequencing without Reference Genome
- Eukaryotic Transcriptome Sequencing with Reference Genome
- Prokaryotic Transcriptome Sequencing Analysis
- LncRNA Sequencing of Plant
- Plant Small RNA Sequencing
- Plant Circular RNA Sequencing
- Plant Comparative Transcriptome Service
- Plant Isoform-sequencing with Reference Genome
- Plant Isoform-sequencing without Reference Genome
- Ribo-seq of Plant
- Metatranscriptome Sequencing of Plant
- Plant Single Cell Level Sequencing Services
- Plant Epigenetics Level Sequencing Services
- Plant Proteomics Service
- Proteomics Qualitative Analysis in Plant
- Plant Protein Quantitative Analysis Service Based on Isotope Labeling (iTRAQ / TMT)
- Non-labeled Plant Protein Quantitative Analysis (Label-free / DIA)
- Plant Protein Targeted Quantitative Service (PRM / MRM / AQUA)
- Post-translational Modification Proteomics (PTMs) Service for Plant
- Plant Metabolomics Services
- Plant DNA-level Sequencing Services
- Other Services
- Plant CNV Analysis Service
- Plant Mutation Detection Service
- Plant Strain / Cell Level Services
- Plant Tissue and Cell Culture Services
- Plant Polyploidization Services
- Plant Haploidization Services
- Plant Phenotypic Analysis
- Plant Stress Response Indicators Analysis
- Plant Biochemical Analysis
- Plant Tissue and Cell Imaging Services
- Plant Disease Identification Services
- Plant Organelle Isolation Services
- Genetically Modified Plant Testing Services
- Seed Testing Services
Plant Genetic Evolution Service
INQUIRYINTRODUCTIONS
The study of population evolution based on whole-genome resequencing can effectively dig out the genetic variation within the population, which can be used to trace the evolution process of the population and dig out evolutionary genes. It has been widely used in many species' artificial domestication mechanism research, natural selection mechanism analysis and population history research. It has important scientific theory and practical application value. It refers to the use of whole-genome resequencing technology to obtain the genome information of each subgroup of a natural population of a species. Through comparison with the reference genome, a large number of highly accurate SNP, InDel, SV, CNV and other mutation information are obtained, and then based on the population Variation information, discuss biological issues such as the genetic structure of the population, gene exchanges, speciation mechanism, and population evolution dynamics, so as to in-depth study of the evolutionary process of the species from the Molecular level.
SERVICES
With years of rich experience, Lifeasible provides plant genetic evolution service. We provide a one-stop service model. We complete a series of processes from sample extraction, quality testing, sequencing, biological information analysis to making reports.
Workflow of plant genetic evolution service
Analysis content
- Mutation detection
Accurately locate mutation sites such as SNP, Indel, CNV, etc. (Tree, PCA, Structure)
- Group structure analysis
Construct a phylogenetic tree, analyze the genetic structure, and determine the evolutionary relationship between samples
- Genetic diversity analysis
Estimation of the θπ, θw polymorphism parameters of the population
- Selective elimination analysis
The selective elimination in the evolution of different populations reveals the key genes related to selection
- Gene function annotation and enrichment analysis
Calculate the distribution of candidate genes and find functional genes related to traits
- Population history analysis
Discuss the history of differentiation between the sample clans
- Gene exchange analysis
Provide ideas for studying migration events in the process of population evolution
Group size
- Usually three subgroups/subspecies or more are required, and each subgroup selects about 10 samples (recommended animals≥10, plants≥15, rare species can appropriately reduce individuals), the overall recommendation is not less than 30 samples.
Project cycle
- The entire project generally takes about 2 months. The specific completion time depends on the specific scale of the project (number of samples and number of phenotype types).
Feedback to customers
- Raw data
- A project report includes experimental process, instrument parameters, analysis methods and results, etc.
Service process
ADVANTAGES
- From material selection, library construction and sequencing, to data analysis, every step has a scientific and meticulous design to ensure high-quality research results.
- Lifeasible has a high-performance computing platform (HPC) that uses an efficient combination of DELL computing nodes and Isilon storage to achieve fast and stable sequencing data analysis and delivery.
- Personalized customized package service.
※ It should be noted that our service is only used for research.
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