Services
- Genetic Marker Assisted Breeding
- Plant Molecular Biology Research
- Research on DNA Level of Plant
- Research on Microspore Embryogenesis
- Plant Genetic Engineering
- Plant Genome Editing with CRISPR / Cas9
- Plant Genome Editing with TALEN
- Plant Genome Editing with ZFN
- RNAi Mediated Plant Gene Silencing
- Overexpression of Plant Genes
- Arabidopsis thaliana Transformation
- Oryza sativa Transformation
- Zea mays Transformation
- Triticum aestivum Transformation
- Medicago truncatula Transformation
- Glycine max Transformation
- Gossypium hirsutum Transformation
- Nicotiana tabacum Transformation
- Solanum lycopersicum Transformation
- Brassica napus Transformation
- Solanum tuberosum Transformation
- Virus-Induced Gene Silencing (VIGS) of Plant Genes
- Research on Protein Level of Plant
- Plant Epigenetic Modification Testing Services
- Sequencing-based Plant Breeding
- Plant DNA-level Sequencing Services
- Plant Genome De Novo Service
- Plant Whole Genome Resequencing Service
- Plant Reduced-Representation Genome Sequencing (RRGS)
- Plant Genetic Map Service
- BSA Trait Positioning of Plant
- Genome-Wide Association Study (GWAS) of Plant
- eQTL Analysis of Plant
- Plant Genetic Evolution Service
- Plant Pan-genome Sequencing
- Plant Whole Exome Sequencing Service
- Individual Selection Pressure Analysis of Plant
- Mixing-tank Selection Pressure Analysis of Plant
- Plant Whole Genome Survey
- Plant RNA Level Sequencing Services
- Eukaryotic Transcriptome Sequencing without Reference Genome
- Eukaryotic Transcriptome Sequencing with Reference Genome
- Prokaryotic Transcriptome Sequencing Analysis
- LncRNA Sequencing of Plant
- Plant Small RNA Sequencing
- Plant Circular RNA Sequencing
- Plant Comparative Transcriptome Service
- Plant Isoform-sequencing with Reference Genome
- Plant Isoform-sequencing without Reference Genome
- Ribo-seq of Plant
- Metatranscriptome Sequencing of Plant
- Plant Single Cell Level Sequencing Services
- Plant Epigenetics Level Sequencing Services
- Plant Proteomics Service
- Proteomics Qualitative Analysis in Plant
- Plant Protein Quantitative Analysis Service Based on Isotope Labeling (iTRAQ / TMT)
- Non-labeled Plant Protein Quantitative Analysis (Label-free / DIA)
- Plant Protein Targeted Quantitative Service (PRM / MRM / AQUA)
- Post-translational Modification Proteomics (PTMs) Service for Plant
- Plant Metabolomics Services
- Plant DNA-level Sequencing Services
- Other Services
- Plant CNV Analysis Service
- Plant Mutation Detection Service
- Plant Strain / Cell Level Services
- Plant Tissue and Cell Culture Services
- Plant Polyploidization Services
- Plant Haploidization Services
- Plant Phenotypic Analysis
- Plant Stress Response Indicators Analysis
- Plant Biochemical Analysis
- Plant Tissue and Cell Imaging Services
- Plant Disease Identification Services
- Plant Organelle Isolation Services
- Genetically Modified Plant Testing Services
- Seed Testing Services
LncRNA Sequencing of Plant
INQUIRYIntroductions
Long non-coding RNA (lncRNA) is a non-coding RNA with unique regulatory functions that has attracted widespread attention in recent years. The length of lncRNA is more than 200nt, does not code for protein, and has poor conservation between species. Some sequence structures are similar to mRNA (containing polyA tails and variable splicing), while others do not have these characteristics. LncRNA regulates gene expression at epigenetic, transcription and post-transcriptional levels by binding to DNA, RNA or protein. This technology has been widely used in the fields of medicine and agronomy, mainly for the search and prediction of new lncRNA, disease research, regulatory network research, etc.
Services
Lifeasible uses the illumina sequencing platform for sequencing, carries out accurate lncRNA identification and target gene prediction of lncRNA for samples with reference genome, and provides analysis of mRNA in sequencing data. The results are more comprehensive and are widely used in medical and agronomic research fields.
Workflow of lncRNA sequencing
Analysis content
Basic analysis
- Standard Information Analysis
- Sequencing data evaluation
- Identification and annotation of known lncRNA
- Transcript reconstruction and novel lncRNA prediction
- Expression level statistics of lncRNA
- Sample relationship analysis of lncRNA
- Difference analysis of lncRNA
- Family analysis of lncRNA
Advanced analysis
- lncRNA location classification (Intergentic lncRNA, Intronic lncRNA, cis-antisense lncRNA)
- Conservation analysis of candidate lncRNA
- Through analysis (need to provide different levels of data of the same sample, such as correlation with sRNA sequencing results)
- Candidate gene analysis (need to specify that it is a certain expression pattern gene, non-coding gene or transcription factor)
- Customized charts (can be made according to the references or requirements provided by the teacher)
- Customized and personalized analysis (plan or reference required)
Sample requirements
- Total sample requirement≥10μg, concentration≥300ng/μl, RIN≥7, OD260/280＝1.8~2.2, OD260/230≥2.0, 28S/18S≥1.0
- Please put the sample in a 1.5ml tube, indicate the sample name, concentration and preparation time on the tube, and use parafilm to seal the tube mouth.
- Before shipping, fix all sample tubes in 50ml centrifuge tubes with caps and ship them on dry ice.
Project cycle
- The entire project generally takes about 2 months.
Feedback to customers
- Raw data
- A project report includes experimental process, instrument parameters, analysis methods and results, etc.
Service process
Advantages
- Lifeasible specializes in providing services related to agricultural molecular breeding, with standardized systems and scientific management.
- From material selection, library construction and sequencing, to data analysis, every step has a scientific and meticulous design to ensure high-quality research results.
- The one-stop service we provide makes your upstream and downstream experiments seamlessly connected, facilitates your experiments more conveniently and quickly.
- The continuously updated and expanded high-performance computing platform ensures efficient data processing and secure data storage.
- Personalized customized package service.
※ For research or industrial use.
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