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Plant Whole Genome Survey

INQUIRY

INTRODUCTIONS

Whole Genome Survey

Genomic Survey is based on low-depth sequencing data of small fragment libraries. Through K-mer analysis, it can effectively evaluate information such as genome size, GC content, heterozygosity, and repetitive sequence content. It is an effective method to fully understand the characteristics of the genome of a certain species, and can provide a theoretical basis for the subsequent formulation of whole-genome de novo sequencing and assembly strategies. Survey is an effective method for evaluating genomes. For species without reference genomes, it is necessary to evaluate genome characteristics before launching the de novo project. The size and complexity of the genome directly affect the project price, cycle, assembly strategy, etc.

SERVICES

With years of rich experience, Lifeasible provides whole genome survey service. We provide a one-stop service model. We complete a series of processes from sample extraction, quality testing, sequencing, biological information analysis to making reports.

Workflow of whole genome survey

Workflow of whole genome survey

Analysis content

Standard analysis

  • Genome size assessment
  • Heterozygosity assessment
  • Repeat rate evaluation
  • Preliminary assembly
  • GC content assessment

Advanced analysis

  • SSR mark development
  • Same origin annotation

Through K-mer analysis, the size of the species' genome is estimated, and the species' genome and repeated sequence situation are analyzed. After the initial assembly of the genome, GC content and exogenous contamination analysis were performed. At the same time, based on the pre-assembled version, SSR marker development and homologous annotation can be carried out.

Project cycle

  • The entire project generally takes about 1 months. The specific completion time depends on the specific scale of the project (number of samples and number of phenotype types).

Feedback to customers

  • Raw data
  • A project report includes experimental process, instrument parameters, analysis methods and results, etc.

Service process

Service process

ADVANTAGES

  • From material selection, library construction and sequencing, to data analysis, every step has a scientific and meticulous design to ensure high-quality research results.
  • Lifeasible has a high-performance computing platform (HPC) that uses an efficient combination of DELL computing nodes and isilon storage to achieve fast and stable sequencing data analysis and delivery.
  • The continuously updated and expanded high-performance computing platform ensures efficient data processing and secure data storage.
  • Personalized customized package service.

※ It should be noted that our service is only used for research.

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