Services
- Genetic Marker Assisted Breeding
- Plant Molecular Biology Research
- Research on DNA Level of Plant
- Research on Microspore Embryogenesis
- Plant Genetic Engineering
- Plant Genome Editing with CRISPR / Cas9
- Plant Genome Editing with TALEN
- Plant Genome Editing with ZFN
- RNAi Mediated Plant Gene Silencing
- Overexpression of Plant Genes
- Arabidopsis thaliana Transformation
- Oryza sativa Transformation
- Zea mays Transformation
- Triticum aestivum Transformation
- Medicago truncatula Transformation
- Glycine max Transformation
- Gossypium hirsutum Transformation
- Nicotiana tabacum Transformation
- Solanum lycopersicum Transformation
- Brassica napus Transformation
- Solanum tuberosum Transformation
- Virus-Induced Gene Silencing (VIGS) of Plant Genes
- Research on Protein Level of Plant
- Plant Epigenetic Modification Testing Services
- Sequencing-based Plant Breeding
- Plant DNA-level Sequencing Services
- Plant Genome De Novo Service
- Plant Whole Genome Resequencing Service
- Plant Reduced-Representation Genome Sequencing (RRGS)
- Plant Genetic Map Service
- BSA Trait Positioning of Plant
- Genome-Wide Association Study (GWAS) of Plant
- eQTL Analysis of Plant
- Plant Genetic Evolution Service
- Plant Pan-genome Sequencing
- Plant Whole Exome Sequencing Service
- Individual Selection Pressure Analysis of Plant
- Mixing-tank Selection Pressure Analysis of Plant
- Plant Whole Genome Survey
- Plant RNA Level Sequencing Services
- Eukaryotic Transcriptome Sequencing without Reference Genome
- Eukaryotic Transcriptome Sequencing with Reference Genome
- Prokaryotic Transcriptome Sequencing Analysis
- LncRNA Sequencing of Plant
- Plant Small RNA Sequencing
- Plant Circular RNA Sequencing
- Plant Comparative Transcriptome Service
- Plant Isoform-sequencing with Reference Genome
- Plant Isoform-sequencing without Reference Genome
- Ribo-seq of Plant
- Metatranscriptome Sequencing of Plant
- Plant Single Cell Level Sequencing Services
- Plant Epigenetics Level Sequencing Services
- Plant Proteomics Service
- Proteomics Qualitative Analysis in Plant
- Plant Protein Quantitative Analysis Service Based on Isotope Labeling (iTRAQ / TMT)
- Non-labeled Plant Protein Quantitative Analysis (Label-free / DIA)
- Plant Protein Targeted Quantitative Service (PRM / MRM / AQUA)
- Post-translational Modification Proteomics (PTMs) Service for Plant
- Plant Metabolomics Services
- Plant DNA-level Sequencing Services
- Other Services
- Plant CNV Analysis Service
- Plant Mutation Detection Service
- Plant Strain / Cell Level Services
- Plant Tissue and Cell Culture Services
- Plant Polyploidization Services
- Plant Haploidization Services
- Plant Phenotypic Analysis
- Plant Stress Response Indicators Analysis
- Plant Biochemical Analysis
- Plant Tissue and Cell Imaging Services
- Plant Disease Identification Services
- Plant Organelle Isolation Services
- Genetically Modified Plant Testing Services
- Seed Testing Services
Plant Dot Blot Hybridization Service
INQUIRYIntroductions
Dot blot hybridization uses the principle of nucleic acid hybridization to detect whether there is nucleic acid hybridization and its hybridization strength. It is mainly used for the detection of plant gene deletion or copy number variation. Its experimental principle is very simple. Firstly, the DNA to be tested is denatured and spotted on a nitrocellulose membrane (or nylon membrane), bake and fix it; Secondly, hybridization is performed with a labeled probe; Then the membrane is washed to remove unattached probes; Finally, autoradiography. This technology is mainly used for the detection of gene deletion or copy number change.
Services
As a well-known plant molecular breeding company, Lifeasible has rich experience in all aspects of molecular breeding, and provides top-notch technical services for the research of nucleic acids, proteins, cells, tissues and other different levels in plant systems. Technical services at the nucleic acid level are designed to help researchers understand the biological functions of plant genes and master these functions to regulate plant growth, stress resistance, disease prevention and treatment, and the cultivation and selection of required plant traits. As part of its trait introgression services, Lifeasible has established a dot blot hybridization platform to meet your related needs and serve you wholeheartedly.
Workflow of dot blot hybridization
Provided by customer
- The name and sequence of the target gene to be tested
- Total RNA (DNA) ≥ 20ug, concentration > 1ug / ul, A260 / A280 > 1.8
- Labeled probe, indicate its concentration and activity (or submit it to Lifeasible for synthesis)
Provided by Lifeasible
- Remaining template and primers
- Image
- Complete project report (materials, reagents, instruments, methods, data analysis, experimental results)
Advantages
- Set up a scientific control group and quickly design probes to ensure a high success rate of experiments.
- Repeatability of experimental results to ensure the accuracy of results.
- Complete information analysis.
- Detailed experimental reports, including materials, reagents, instruments, methods, data analysis, experimental results, etc.
- Provide clear experimental pictures.
- Quality after-sales service.
※ It should be noted that our service is only used for research.
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