Services
- Genetic Marker Assisted Breeding
- Plant Molecular Biology Research
- Research on DNA Level of Plant
- Research on Microspore Embryogenesis
- Plant Genetic Engineering
- Plant Genome Editing with CRISPR / Cas9
- Plant Genome Editing with TALEN
- Plant Genome Editing with ZFN
- RNAi Mediated Plant Gene Silencing
- Overexpression of Plant Genes
- Arabidopsis thaliana Transformation
- Oryza sativa Transformation
- Zea mays Transformation
- Triticum aestivum Transformation
- Medicago truncatula Transformation
- Glycine max Transformation
- Gossypium hirsutum Transformation
- Nicotiana tabacum Transformation
- Solanum lycopersicum Transformation
- Brassica napus Transformation
- Solanum tuberosum Transformation
- Virus-Induced Gene Silencing (VIGS) of Plant Genes
- Research on Protein Level of Plant
- Plant Epigenetic Modification Testing Services
- Sequencing-based Plant Breeding
- Plant DNA-level Sequencing Services
- Plant Genome De Novo Service
- Plant Whole Genome Resequencing Service
- Plant Reduced-Representation Genome Sequencing (RRGS)
- Plant Genetic Map Service
- BSA Trait Positioning of Plant
- Genome-Wide Association Study (GWAS) of Plant
- eQTL Analysis of Plant
- Plant Genetic Evolution Service
- Plant Pan-genome Sequencing
- Plant Whole Exome Sequencing Service
- Individual Selection Pressure Analysis of Plant
- Mixing-tank Selection Pressure Analysis of Plant
- Plant Whole Genome Survey
- Plant RNA Level Sequencing Services
- Eukaryotic Transcriptome Sequencing without Reference Genome
- Eukaryotic Transcriptome Sequencing with Reference Genome
- Prokaryotic Transcriptome Sequencing Analysis
- LncRNA Sequencing of Plant
- Plant Small RNA Sequencing
- Plant Circular RNA Sequencing
- Plant Comparative Transcriptome Service
- Plant Isoform-sequencing with Reference Genome
- Plant Isoform-sequencing without Reference Genome
- Ribo-seq of Plant
- Metatranscriptome Sequencing of Plant
- Plant Single Cell Level Sequencing Services
- Plant Epigenetics Level Sequencing Services
- Plant Proteomics Service
- Proteomics Qualitative Analysis in Plant
- Plant Protein Quantitative Analysis Service Based on Isotope Labeling (iTRAQ / TMT)
- Non-labeled Plant Protein Quantitative Analysis (Label-free / DIA)
- Plant Protein Targeted Quantitative Service (PRM / MRM / AQUA)
- Post-translational Modification Proteomics (PTMs) Service for Plant
- Plant Metabolomics Services
- Plant DNA-level Sequencing Services
- Other Services
- Plant CNV Analysis Service
- Plant Mutation Detection Service
- Plant Strain / Cell Level Services
- Plant Tissue and Cell Culture Services
- Plant Polyploidization Services
- Plant Haploidization Services
- Plant Phenotypic Analysis
- Plant Stress Response Indicators Analysis
- Plant Biochemical Analysis
- Plant Tissue and Cell Imaging Services
- Plant Disease Identification Services
- Plant Organelle Isolation Services
- Genetically Modified Plant Testing Services
- Seed Testing Services
BSA Trait Positioning of Plant
INQUIRYINTRODUCTIONS
Bulked-Segregant Analysis (BSA), also known as QTL-seq, is used for gene mapping of qualitative traits or quantitative traits with major genes, and has a wide range of applications in genetics and breeding. First, select two parents with extreme differences in phenotypic traits to construct a family, and perform whole-genome resequencing on the two sample pools obtained by mixing the offspring with extreme phenotypes of the target traits in this family. The detected DNA difference fragments between the two pools are the candidate regions. By calculating the difference between the genotype frequencies of the two mixed pools, the region where the QTL related to the trait is located can be determined. Further combining with the mutation information of the coding genes in this interval, candidate genes and candidate functional mutations for controlling traits can be determined.
SERVICES
With years of rich experience, Lifeasible provides BSA trait positioning service. We provide a one-stop service model. We complete a series of processes from sample extraction, quality testing, sequencing, biological information analysis to making reports.
Workflow of BSA trait positioning service
Analysis content
- Sequencing data quality assessment (base composition distribution, etc.)
- Raw data filtering to remove reads containing connectors and low-quality reads
- Align with reference genome
Aligned genome statistics
Insert Fragment Statistics
Analysis of gene coverage
In-depth analysis of sequencing
- SNP analysis statistics
SNP detection
SNP position and function annotation
SNP conversion and transversion information statistics
SNP heterozygous information statistics
- InDel analysis statistics
InDel detection
InDel location and function notes
InDel heterozygous information statistics
- BSA analysis
Comparison of polymorphism between parents
SNP frequency analysis of offspring (calculation of SNP index and ΔSNP index)
Target traits related area positioning
Analysis of candidate genes in the target region
Sample requirements
- Sample concentration≥300ng/μl, total amount≥6μg, OD260/280＝1.8~2.0.
(The amount of DNA required is related to the number of libraries constructed and the type of library)
Project cycle
- The entire project generally takes about 2 months.
Feedback to customers
- Raw data
- A project report includes experimental process, instrument parameters, analysis methods and results, etc.
Service process
ADVANTAGES
- Our experienced researchers help you with scientific plan design.
- The advanced illumina sequencing platform is used to quickly and efficiently read high-quality sequencing data.
- BSA can be used for the initial gene mapping of qualitative traits or quantitative traits with major genes (for example, plant disease resistance), or the study of single traits.
- With the advantages of high speed, high density, and high quality, it has been widely used in the construction of genetic maps of various model and non-model organisms.
- Personalized customized package service.
※ It should be noted that our service is only used for research.
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