Services

10x Genomics Single Cell ATAC Sequencing of Plant

INQUIRY

Introductions

10xGenomics Single Cell ATAC Sequencing

10x Gennomics single cell ATAC-seq (single cell assay for transposase accessible chromatin with high-throughput sequencing, 10x scATAC-seq) is a research strategy that reveals chromatin accessibility at the level of single cell epigenomics. Constructing a single-cell chromatin accessibility map can not only clearly understand the chromatin structure, but also map a high-resolution transcription factor regulatory network. It is an important breakthrough in single-cell epigenetics by studying epigenetic regulation mechanisms while addressing cell heterogeneity, and combining motif analysis to identify transcription factors involved in gene expression regulation. This technology has been widely used in various fields of medical and agricultural molecular breeding, such as studying the classification of cell subgroups, identifying rare cell types, studying pathological diagnosis, screening specific molecular markers, and so on.

Services

With years of rich experience, Lifeasible provides 10xGenomics single cell ATAC sequencing and analysis service. Customers only need to provide tissues or single cell nuclear suspension, and we complete the entire service process from sample extraction, quality testing, sequencing, biological information analysis to making reports.

Workflow of 10xGenomics single cell ATAC sequencing

Workflow of 10xGenomics single cell ATAC sequencing

Analysis content

Analysis content of 10xGenomics single cell ATAC sequencing

Sample requirements

  • Sample type
  • Single cell nuclear suspension, fresh tissue

  • Cell types
  • Cell lines, primary cells, cryopreserved samples

  • Nucleus size
  • Less than 40μm

  • Data volume
  • It is recommended to measure 25k read pairs per cell nucleus.

Project cycle

  • It takes 60 working days to complete the standard process. 

Feedback to customers

  • Raw data
  • A project report includes experimental process, instrument parameters, analysis methods and results, etc.

Service process

Service process of 10xGenomics single cell ATAC sequencing

Advantages

  • Lifeasible specializes in providing services related to agricultural molecular breeding, with standardized systems and scientific management.
  • The experiment adopts a carefully optimized experimental procedure, which has extremely high efficiency and specificity.
  • Strict quality control is carried out on each key step of the experiment, and the quality of the experiment is monitored throughout the process to ensure that customers receive high-quality data.
  • With a strong bioinformatics team, it can meet the various in-depth data analysis needs of customers.
  • The one-stop service we provide makes your upstream and downstream experiments seamlessly connected, facilitates your experiments more conveniently and quickly.
  • Personalized customized package service.

※ For research or industrial use.

Online Inquiry