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Individual Selection Pressure Analysis of Plant

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INTRODUCTIONS

Individual Selection Pressure Analysis

Individual selection pressure analysis is a population genetic analysis based on individual samples. Its essence is to use resequencing to obtain the SNP variation information of each individual sample in each subspecies (or strain) population of a species. Through SNP, each subspecies (strain) can be analyzed. Genetic evolutionary relationship between. The individual sample strategy can obtain more accurate individual genetic information, so through the selection pressure analysis, not only the adaptive mechanism of the group can be studied, but also the group relationship can be analyzed. Therefore, this technology is mainly used in many aspects of research, such as the study of natural selection mechanism of species under different living conditions, the study of artificial selection mechanism of wild, domesticated and improved species, the study of population history and evolution.

SERVICES

With years of rich experience, Lifeasible provides individual selection pressure analysis service. We provide a one-stop service model. We complete a series of processes from sample extraction, quality testing, sequencing, biological information analysis to making reports.

Technical route

Technical route

Analysis content

  • SNP marker screening and filtering
  • Group structure analysis
  • LD attenuation distance analysis
  • Choose clear analysis
  • Selected regions analysis (reference genome must be present)

Sample requirements

  • Whole genome resequencing

Sample concentration≥30ng/ul, total amount≥6μg, OD260/280=1.8~2.0

  • Simplified genome sequencing

Sample concentration≥25ng/μl, total amount≥2μg, OD260/280=1.8~2.0

  • Recommended number of samples

It is recommended that each group have more than 10 samples. If the number of samples reaches 30, the calculation of various indicators of group diversity will be more accurate.

Project cycle

  • The entire project generally takes about 2~3 months, and the specific completion time depends on samples number and phenotype types.

Feedback to customers

  • Raw data
  • A project report includes experimental process, instrument parameters, analysis methods and results, etc.

Service process

Service process

ADVANTAGES

  • From material selection, library construction and sequencing, to data analysis, every step has a scientific and meticulous design to ensure high-quality research results.
  • Lifeasible has a high-performance computing platform (HPC) that uses an efficient combination of DELL computing nodes and isilon storage to achieve fast and stable sequencing data analysis and delivery.
  • The continuously updated and expanded high-performance computing platform ensures efficient data processing and secure data storage.
  • Personalized customized package service.

※ It should be noted that our service is only used for research.

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