Services
- Genetic Marker Assisted Breeding
- Plant Molecular Biology Research
- Research on DNA Level of Plant
- Research on Microspore Embryogenesis
- Plant Genetic Engineering
- Plant Genome Editing with CRISPR / Cas9
- Plant Genome Editing with TALEN
- Plant Genome Editing with ZFN
- RNAi Mediated Plant Gene Silencing
- Overexpression of Plant Genes
- Arabidopsis thaliana Transformation
- Oryza sativa Transformation
- Zea mays Transformation
- Triticum aestivum Transformation
- Medicago truncatula Transformation
- Glycine max Transformation
- Gossypium hirsutum Transformation
- Nicotiana tabacum Transformation
- Solanum lycopersicum Transformation
- Brassica napus Transformation
- Solanum tuberosum Transformation
- Virus-Induced Gene Silencing (VIGS) of Plant Genes
- Research on Protein Level of Plant
- Plant Epigenetic Modification Testing Services
- Sequencing-based Plant Breeding
- Plant DNA-level Sequencing Services
- Plant Genome De Novo Service
- Plant Whole Genome Resequencing Service
- Plant Reduced-Representation Genome Sequencing (RRGS)
- Plant Genetic Map Service
- BSA Trait Positioning of Plant
- Genome-Wide Association Study (GWAS) of Plant
- eQTL Analysis of Plant
- Plant Genetic Evolution Service
- Plant Pan-genome Sequencing
- Plant Whole Exome Sequencing Service
- Individual Selection Pressure Analysis of Plant
- Mixing-tank Selection Pressure Analysis of Plant
- Plant Whole Genome Survey
- Plant RNA Level Sequencing Services
- Eukaryotic Transcriptome Sequencing without Reference Genome
- Eukaryotic Transcriptome Sequencing with Reference Genome
- Prokaryotic Transcriptome Sequencing Analysis
- LncRNA Sequencing of Plant
- Plant Small RNA Sequencing
- Plant Circular RNA Sequencing
- Plant Comparative Transcriptome Service
- Plant Isoform-sequencing with Reference Genome
- Plant Isoform-sequencing without Reference Genome
- Ribo-seq of Plant
- Metatranscriptome Sequencing of Plant
- Plant Single Cell Level Sequencing Services
- Plant Epigenetics Level Sequencing Services
- Plant Proteomics Service
- Proteomics Qualitative Analysis in Plant
- Plant Protein Quantitative Analysis Service Based on Isotope Labeling (iTRAQ / TMT)
- Non-labeled Plant Protein Quantitative Analysis (Label-free / DIA)
- Plant Protein Targeted Quantitative Service (PRM / MRM / AQUA)
- Post-translational Modification Proteomics (PTMs) Service for Plant
- Plant Metabolomics Services
- Plant DNA-level Sequencing Services
- Other Services
- Plant CNV Analysis Service
- Plant Mutation Detection Service
- Plant Strain / Cell Level Services
- Plant Tissue and Cell Culture Services
- Plant Polyploidization Services
- Plant Haploidization Services
- Plant Phenotypic Analysis
- Plant Stress Response Indicators Analysis
- Plant Biochemical Analysis
- Plant Tissue and Cell Imaging Services
- Plant Disease Identification Services
- Plant Organelle Isolation Services
- Genetically Modified Plant Testing Services
- Seed Testing Services
Plant Promoter Research Services
INQUIRYIntroductions
In the growth and development of organisms, specific genes are expressed regularly, positioned, and quantitatively according to themselves and the environment. This ability is achieved through the interaction of biological DNA cis-acting elements and trans-acting factors, heat and light in the environment, etc. Among them, the role of the promoter is particularly critical. Therefore, the in-depth study of its structure and function is not only of great significance for understanding the growth and development mechanism and defense mechanism of plants, but also provides an effective way for the use of transgenic plant engineering technology to achieve effective and specific gene expression.
Services
With the development of life sciences and the continuous improvement of research methods, more and more technical methods can be applied to the analysis and research of promoters. Lifeasible provides a variety of representative promoter research services.
Service content
Promoter and protein interaction analysisYeast one-hybrid system, co-immunoprecipitation, promoter chip, gel electrophoresis and DNase I footprinting can all be used to analyze the interaction between promoter and protein.
- EMSA
- Co-IP
- Promoter chip
- DNase I footprinting
- Dual luciferase reporter assay, DLR
- Yeast one-hybrid, Y1H
To verify the activity of the cloned promoter, it is necessary to use transgenic technology to make it enter the cell and express the reporter gene, such as luciferase gene, etc., and then detect the transient or stable expression of the reporter gene to evaluate the promoter activity.
- Luciferase reporter gene detection
- GUS staining and detection
Sample requirements
- Different project has different requirements, please contact us for specific information.
Delivery standard
- Project report
- Images
Electronic word document
Electronic version of pictures in JPG or TIF format
Service process
- Service inquiry and technical consultation
- Design experiment
- Sign contracts and confidentiality agreements with customers
- Update project status
- Organize experimental data and pictures, analyze the results and make a report
Project cycle
- It takes about 1~2 months. The specific time required for different projects is different, please contact us for details.
Advantages
- Discuss with you the details of the expected interaction study, and develop experimental strategies / methods according to your requirements.
- Both Promoter and protein interaction analysis and Promoter activity detection have mature technology platforms, which provide you with one-stop services and seamlessly connect your upstream and downstream experiments.
- When detecting the promoter activity, it can be selected according to the experimental purpose and the transfected object. The transient expression operation is simple, fast, safe and effective; stable expression usually requires screening of positive clones through resistance markers, which takes a long time, and is more suitable for some studies that require induced expression of genes or have a long duration.
- Leading technology platform conditions, first-class R&D and production equipment.
- Flexible quotation to meet your needs.
※ It should be noted that our service is only used for research.
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