Services
- Genetic Marker Assisted Breeding
- Plant Molecular Biology Research
- BBM-BAR1-Mediated In Vivo Efficient Haploid Induction Service
- Research on DNA Level of Plant
- Research on Microspore Embryogenesis
- Plant Genetic Engineering
- Plant Genome Editing with CRISPR / Cas9
- Plant Genome Editing with TALEN
- Plant Genome Editing with ZFN
- RNAi Mediated Plant Gene Silencing
- Overexpression of Plant Genes
- Arabidopsis thaliana Transformation
- Oryza sativa Transformation
- Zea mays Transformation
- Triticum aestivum Transformation
- Medicago truncatula Transformation
- Glycine max Transformation
- Gossypium hirsutum Transformation
- Nicotiana tabacum Transformation
- Solanum lycopersicum Transformation
- Brassica napus Transformation
- Solanum tuberosum Transformation
- Virus-Induced Gene Silencing (VIGS) of Plant Genes
- Research on Protein Level of Plant
- Plant Epigenetic Modification Testing Services
- Sequencing-based Plant Breeding
- Plant DNA-level Sequencing Services
- Plant Exon Capture Services
- Wheat Exome Capture Sequencing Service
- Plant Genome De Novo Service
- Plant Whole Genome Resequencing Service
- Plant Reduced-Representation Genome Sequencing (RRGS)
- Plant Genetic Map Service
- BSA Trait Positioning of Plant
- Genome-Wide Association Study (GWAS) of Plant
- eQTL Analysis of Plant
- Plant Genetic Evolution Service
- Plant Pan-genome Sequencing
- Plant Whole Exome Sequencing Service
- Individual Selection Pressure Analysis of Plant
- Mixing-tank Selection Pressure Analysis of Plant
- Plant Whole Genome Survey
- Plant RNA Level Sequencing Services
- Eukaryotic Transcriptome Sequencing without Reference Genome
- Eukaryotic Transcriptome Sequencing with Reference Genome
- Prokaryotic Transcriptome Sequencing Analysis
- LncRNA Sequencing of Plant
- Plant Small RNA Sequencing
- Plant Circular RNA Sequencing
- Plant Comparative Transcriptome Service
- Plant Isoform-sequencing with Reference Genome
- Plant Isoform-sequencing without Reference Genome
- Ribo-seq of Plant
- Metatranscriptome Sequencing of Plant
- Plant Single Cell Level Sequencing Services
- Plant Epigenetics Level Sequencing Services
- Plant Proteomics Service
- Proteomics Qualitative Analysis in Plant
- Plant Protein Quantitative Analysis Service Based on Isotope Labeling (iTRAQ / TMT)
- Non-labeled Plant Protein Quantitative Analysis (Label-free / DIA)
- Plant Protein Targeted Quantitative Service (PRM / MRM / AQUA)
- Post-translational Modification Proteomics (PTMs) Service for Plant
- Plant Metabolomics Services
- Plant DNA-level Sequencing Services
- Other Services
- Plant CNV Analysis Service
- Plant Mutation Detection Service
- Plant Strain / Cell Level Services
- Plant Tissue and Cell Culture Services
- Plant Polyploidization Services
- Plant Haploidization Services
- Plant Phenotypic Analysis
- Plant Stress Response Indicators Analysis
- Plant Biochemical Analysis
- Plant Tissue and Cell Imaging Services
- Plant Disease Identification Services
- Plant Organelle Isolation Services
- Genetically Modified Plant Testing Services
- Seed Testing Services
Plant Exon Capture Services
INQUIRYExon capture is a selectively isolatetechnique designed to selectively isolate protein-coding sequences (exonic regions) from complex genomes. It leverages the RNA splicing capability of exogenous DNA to integrate and capture target exons into known genomic fragments, thereby effectively identifying potential genes.
At Lifeasible, we continuously refine and enhance our exon trapping technology platform to ensure the delivery of more advanced and reliable plant exon trapping services. Leveraging our state-of-the-art, cutting-edge hybridization-based trapping sequencing technology and advanced synthesis platform provide comprehensive whole-exome sequencing solutions for diverse plant species. To date, our exon trapping services cover dozens of plant species, including wheat, barley, Pinus tabulaeformis, and maize.
Advantages of Plant Exon Capture
- High coverage and accuracy. Target regions exhibit strong uniform coverage, reaching depths of 11× or higher, enabling precise detection of variations such as SNPs and Indels.
- Efficiency and cost-effectiveness. Compared to whole-genome sequencing, exome capture targets only 1%-5% of coding regions, reducing data volume by over 90% and significantly lowering sequencing costs and data analysis workload.
- Cross-species applicability. Probes can be used across related species, making them particularly suitable for large-scale population studies and research on non-model plants.
Our Service for Plant Exon Capture
Plant exon capture is a technique for targeted enrichment and sequencing of exon regions within plant genomes. It utilizes designed probes or primers to selectively capture exon sequences, combined with high-throughput sequencing technology to efficiently obtain genetic information from coding regions. Our service effectively screens coding regions from long genomic DNA fragments, particularly suited for incompletely annotated genomic regions. We offer an integrated workflow covering sample preparation, library construction, exon enrichment, high-coverage sequencing, and bioinformatics analysis for identifying genetic variations. These services prove invaluable for crop improvement, disease resistance studies, and genetic diversity research. Below are the core components of this technology.
- Cloning and vector construction. The genomic DNA fragment to be analyzed is cloned into a modified vector. This vector typically carries a splicing donor site (SD) and a multiple cloning site (MCS), where the target DNA fragment is inserted.
- Transfection and expression. The constructed vector is transfected into target plant cells for transcription and splicing. If the inserted DNA fragment contains a functional splice acceptor site (SA), splicing occurs during transcription, removing introns.
- Exon capture. The transcribed RNA undergoes splicing and is reverse transcribed into cDNA, carrying the captured exon. These cDNAs are recovered via PCR amplification or other methods to analyze the connection between the exon and the known donor site.
- Screening and analysis. Exon capture events are validated using DNA sequencing or hybridization method. True exon capture events demonstrate the correct connection of a specific exon to its donor site, indicating that the exon may be part of a gene.
Which Species are Covered?
| Wheat | Pine | Maize | Soubean |
| Rice | Chili | Pumpkin | More species |
Fig.2 The types of plants we service. (Lifeasible)
Applications of Plant Exon Capture
- Accelerate crop genetic improvement. Rapidly conduct gene mapping, functional gene discovery, and trait association analysis.
- Molecular breeding and BSA mapping. Rapidly screen superior variants using exome chips to assist breeding design.
- Population genetics analysis. Utilize exome trapping sequencing for large-scale natural population sequencing to identify genetic variants associated with target traits.
- Gene cloning and functional studies. Precisely locate candidate genes for specific agronomic traits.
Our Technical Service Process
- Sample receipt and quality control. Receive client samples and conduct rigorous quality assessment.
- Library preparation. Randomly fragment qualified DNA samples into specific-sized pieces, followed by end repair, A-tailing, and sequencing adapter ligation.
- Target enrichment/hybridization. Liquid-phase hybridization of the library with pre-designed biotin-labeled probes to specifically capture target DNA fragments.
- Enrichment and amplification. Target DNA libraries bound to probes are eluted and amplified via PCR.
- Sequencing. The amplified captured library undergoes quality assessment; qualified samples proceed to high-throughput sequencing.
- Bioinformatics analysis. Raw sequencing data undergoes quality control, alignment, variant detection, annotation, and statistical analysis.
Highlights of Our Plant Exon Capture Services
- Synthetic advantages. Leveraging our advanced large-scale chip oligonucleotide library synthesis platform, we rapidly generate millions of oligonucleotide libraries. Furthermore, we have assisted scientists in developing whole-exome chips for over a hundred species.
- Superior data performance. Combined with our advanced capture systems, we consistently deliver exceptional target coverage and consistency.
- Flexible customization. We provide tailored whole-exome or breeding chips for various species, offering comprehensive solutions including extraction, library preparation, capture kits, and automated equipment.
- Collaborative development. We employ flexible partnership models to jointly develop whole-exome products and solutions for diverse plant species, maximizing alignment with client research objectives.
Lifeasible specializes in providing one-stop technical services for your plant exome capture projects, delivering advanced and reliable solutions tailored to your research needs. If you are interested, please feel free to contact us.
※ For research or industrial use.
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