Services
- Genetic Marker Assisted Breeding
- Plant Molecular Biology Research
- Research on DNA Level of Plant
- Research on Microspore Embryogenesis
- Plant Genetic Engineering
- Plant Genome Editing with CRISPR / Cas9
- Plant Genome Editing with TALEN
- Plant Genome Editing with ZFN
- RNAi Mediated Plant Gene Silencing
- Overexpression of Plant Genes
- Arabidopsis thaliana Transformation
- Oryza sativa Transformation
- Zea mays Transformation
- Triticum aestivum Transformation
- Medicago truncatula Transformation
- Glycine max Transformation
- Gossypium hirsutum Transformation
- Nicotiana tabacum Transformation
- Solanum lycopersicum Transformation
- Brassica napus Transformation
- Solanum tuberosum Transformation
- Virus-Induced Gene Silencing (VIGS) of Plant Genes
- Research on Protein Level of Plant
- Plant Epigenetic Modification Testing Services
- Sequencing-based Plant Breeding
- Plant DNA-level Sequencing Services
- Plant Genome De Novo Service
- Plant Whole Genome Resequencing Service
- Plant Reduced-Representation Genome Sequencing (RRGS)
- Plant Genetic Map Service
- BSA Trait Positioning of Plant
- Genome-Wide Association Study (GWAS) of Plant
- eQTL Analysis of Plant
- Plant Genetic Evolution Service
- Plant Pan-genome Sequencing
- Plant Whole Exome Sequencing Service
- Individual Selection Pressure Analysis of Plant
- Mixing-tank Selection Pressure Analysis of Plant
- Plant Whole Genome Survey
- Plant RNA Level Sequencing Services
- Eukaryotic Transcriptome Sequencing without Reference Genome
- Eukaryotic Transcriptome Sequencing with Reference Genome
- Prokaryotic Transcriptome Sequencing Analysis
- LncRNA Sequencing of Plant
- Plant Small RNA Sequencing
- Plant Circular RNA Sequencing
- Plant Comparative Transcriptome Service
- Plant Isoform-sequencing with Reference Genome
- Plant Isoform-sequencing without Reference Genome
- Ribo-seq of Plant
- Metatranscriptome Sequencing of Plant
- Plant Single Cell Level Sequencing Services
- Plant Epigenetics Level Sequencing Services
- Plant Proteomics Service
- Proteomics Qualitative Analysis in Plant
- Plant Protein Quantitative Analysis Service Based on Isotope Labeling (iTRAQ / TMT)
- Non-labeled Plant Protein Quantitative Analysis (Label-free / DIA)
- Plant Protein Targeted Quantitative Service (PRM / MRM / AQUA)
- Post-translational Modification Proteomics (PTMs) Service for Plant
- Plant Metabolomics Services
- Plant DNA-level Sequencing Services
- Other Services
- Plant CNV Analysis Service
- Plant Mutation Detection Service
- Plant Strain / Cell Level Services
- Plant Tissue and Cell Culture Services
- Plant Polyploidization Services
- Plant Haploidization Services
- Plant Phenotypic Analysis
- Plant Stress Response Indicators Analysis
- Plant Biochemical Analysis
- Plant Tissue and Cell Imaging Services
- Plant Disease Identification Services
- Plant Organelle Isolation Services
- Genetically Modified Plant Testing Services
- Seed Testing Services
Plant Northern Blot Hybridization Service
INQUIRYIntroductions
Northern blot hybridization uses the principle of nucleic acid hybridization, and can observe the size and abundance of specific genes in plant tissues, organs, developmental stages, adversity, pathogen infection or during the entire treatment process with high precision and accuracy. The principle of northern blot hybridization is very simple. Firstly, according to the relative molecular weight, the total RNA is separated by denaturing agarose gel or polyacrylamide gel electrophoresis. Secondly, transfer the separated northern blot to nylon membrane or nitrocellulose membrane. Finally, the northern blot is hybridized with the labeled probe. If a gene is found to be significantly up-regulated or down-regulated, sequencing can be used to determine whether the gene is known or newly discovered. Electrophoretic separation based on molecular size reflects alternative splicing products of the same gene or repeated sequence. The size of the gene product can reflect the deletion or error in the transcription process. And by changing the probe with a known sequence, the region of RNA deletion can be determined.
Services
Lifeasible is the world's leading agricultural molecular breeding company, able to provide a variety of first-class agricultural biotechnology, including nucleic acid, protein, plant and other levels of research. Technical services at the nucleic acid level are designed to help researchers understand the biological functions of plant genes and master these functions to regulate plant growth, stress resistance, disease prevention and treatment, and the cultivation and selection of required plant traits. As part of its trait introgression services, Lifeasible has established a northern hybridization platform, which can meet your relevant needs and serve you wholeheartedly.
Workflow of northern blot hybridization
Schematic of Northern blot hybridization (Hamad A F, 2017)
Provided by customer
- The target gene sequence and primer sequence to be hybridized
- High-quality RNA samples to be hybridized
- For long-distance transportation, add trozol or 1/10 volume of 3M NaAc pH5.2 to the qualified RNA solution, then add 2 times volume of absolute ethanol, and mix well.
Provided by Lifeasible
- Remaining template and primers
- RNA sample test report (the remaining experimental samples will not be returned, please keep a backup)
- Experiment report (detailed experiment procedure, image, result analysis, etc.)
Advantages
- The probe is labeled with DIG (Digoxin), which has the advantages of low noise, high sensitivity, and no radioactive contamination.
- The original data and experimental materials are retained during the experiment, and the data is true and credible.
- The experimental results are stable and repeatable.
- Combining traditional film exposure with a cutting-edge automated chemiluminescence imaging detection system ensures that imaging time points with a better signal-to-noise ratio are captured, and high-quality experimental results can be delivered to customers. Customers can directly use the experimental data provided by us to submit articles.
Reference
- Hamad A F, Jeong H Y, Han J H, et al. Detection of cytosolic tRNA in mammal by Northern blot analysis[J]. Bangladesh Journal of Pharmacology, 2017, 12(3):243.
※ It should be noted that our service is only used for research.
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