Plant Protein Quantitative Analysis Service Based on Isotope Labeling (iTRAQ / TMT)

INQUIRY

Introductions

iTRAQ and TMT are labeling and quantitative techniques in vitro, developed by AB Sciex and Thermo, respectively. They use 8 or 10 isotope tags to specifically label the amino acid groups of polypeptides to achieve relative quantification of proteins in 8 or 10 different samples at a time. They are a high-throughput screening technology commonly used in quantitative proteomics in recent years. Protein labeling quantitative technology has the advantages of mature technology, wide application, small system error, and low difficulty in experiment and data analysis. Therefore, it has been widely used in protein quantitative analysis. In the field of agricultural molecular breeding, it is mainly applied to protein expression changes in samples under different treatments / different physiological conditions, and protein regulatory network research and other directions, to assist breeders in the selection of high-protein crops.

Services

Technical characteristics

• High throughput, not limited by the number of samples

It can simultaneously satisfy the identification and quantification of protein in 8 samples (iTRAQ) or 10 samples (TMT) at a time. If the number of samples exceeds, we can set internal parameters to form a bridge to achieve multiple detection, so it is not limited by the number of samples.

• Accurate quantification and high repeatability

Different samples are mixed after labeling, and then tested after unified processing, which improves the accuracy and repeatability of quantification.

• Wide range of protein detection

No species-specific restrictions, theoretically applicable to all species.

Experimental procedure for protein labeling quantification

Analysis content

Standard information analysis	Data output statistics
	Protein database establishment and PEAKS DB software search
	Protein identification and quantification
	Protein function annotation (GO function, KEGG metabolic pathway)
	Advanced protein annotation (protein domain annotation, transcription factor annotation, etc.)
	Sample relationship analysis
	Differential protein analysis
	Differential protein GO/KEGG function enrichment analysis
	GSEA analysis
Customized information analysis	Proteome and transcriptome association analysis
	Protein interaction network analysis

Sample requirements

• Fresh plant tissue > 2 g
• The total amount of protein in the protein solution > 500 μg

Project cycle

• It takes 50~70 working days to complete the standard process.

Feedback to customers

• Raw data
• A project report includes experimental procedures, related mass spectrometry parameters, and standard analysis results.

Service process

Advantages

• Lifeasible specializes in providing services related to agricultural molecular breeding, with standardized systems and scientific management.
• The experiment adopts a carefully optimized experimental procedure, which has extremely high efficiency and specificity.
• The one-stop service we provide makes your upstream and downstream experiments seamlessly connected, facilitates your experiments more conveniently and quickly.
• With a strong bioinformatics team, it can meet the various in-depth data analysis needs of customers.
• Personalized customized package service.

 

※ It should be noted that our service is only used for research.

Related Sections

• Proteomics Qualitative Analysis in Plant
• Non-labeled Plant Protein Quantitative Analysis (Label-free / DIA)
• Plant Protein Targeted Quantitative Service (PRM / MRM / AQUA)
• Post-translational Modification Proteomics (PTMs) Service for Plant

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