Services
- Genetic Marker Assisted Breeding
- Plant Molecular Biology Research
- Research on DNA Level of Plant
- Research on Microspore Embryogenesis
- Plant Genetic Engineering
- Plant Genome Editing with CRISPR / Cas9
- Plant Genome Editing with TALEN
- Plant Genome Editing with ZFN
- RNAi Mediated Plant Gene Silencing
- Overexpression of Plant Genes
- Arabidopsis thaliana Transformation
- Oryza sativa Transformation
- Zea mays Transformation
- Triticum aestivum Transformation
- Medicago truncatula Transformation
- Glycine max Transformation
- Gossypium hirsutum Transformation
- Nicotiana tabacum Transformation
- Solanum lycopersicum Transformation
- Brassica napus Transformation
- Solanum tuberosum Transformation
- Virus-Induced Gene Silencing (VIGS) of Plant Genes
- Research on Protein Level of Plant
- Plant Epigenetic Modification Testing Services
- Sequencing-based Plant Breeding
- Plant DNA-level Sequencing Services
- Plant Genome De Novo Service
- Plant Whole Genome Resequencing Service
- Plant Reduced-Representation Genome Sequencing (RRGS)
- Plant Genetic Map Service
- BSA Trait Positioning of Plant
- Genome-Wide Association Study (GWAS) of Plant
- eQTL Analysis of Plant
- Plant Genetic Evolution Service
- Plant Pan-genome Sequencing
- Plant Whole Exome Sequencing Service
- Individual Selection Pressure Analysis of Plant
- Mixing-tank Selection Pressure Analysis of Plant
- Plant Whole Genome Survey
- Plant RNA Level Sequencing Services
- Eukaryotic Transcriptome Sequencing without Reference Genome
- Eukaryotic Transcriptome Sequencing with Reference Genome
- Prokaryotic Transcriptome Sequencing Analysis
- LncRNA Sequencing of Plant
- Plant Small RNA Sequencing
- Plant Circular RNA Sequencing
- Plant Comparative Transcriptome Service
- Plant Isoform-sequencing with Reference Genome
- Plant Isoform-sequencing without Reference Genome
- Ribo-seq of Plant
- Metatranscriptome Sequencing of Plant
- Plant Single Cell Level Sequencing Services
- Plant Epigenetics Level Sequencing Services
- Plant Proteomics Service
- Proteomics Qualitative Analysis in Plant
- Plant Protein Quantitative Analysis Service Based on Isotope Labeling (iTRAQ / TMT)
- Non-labeled Plant Protein Quantitative Analysis (Label-free / DIA)
- Plant Protein Targeted Quantitative Service (PRM / MRM / AQUA)
- Post-translational Modification Proteomics (PTMs) Service for Plant
- Plant Metabolomics Services
- Plant DNA-level Sequencing Services
- Other Services
- Plant CNV Analysis Service
- Plant Mutation Detection Service
- Plant Strain / Cell Level Services
- Plant Tissue and Cell Culture Services
- Plant Polyploidization Services
- Plant Haploidization Services
- Plant Phenotypic Analysis
- Plant Stress Response Indicators Analysis
- Plant Biochemical Analysis
- Plant Tissue and Cell Imaging Services
- Plant Disease Identification Services
- Plant Organelle Isolation Services
- Genetically Modified Plant Testing Services
- Seed Testing Services
Plant Pan-genome Sequencing
INQUIRYINTRODUCTIONS
Pan-genome analysis can be performed regardless of whether there is a reference genome. Compared with a single genome de novo, the research of pan-genome is more comprehensive and in-depth. Pan-genome sequencing is the low/high-depth sequencing, assembly and annotation of multiple species. By constructing a pan-genome map, all genes of this species can be obtained. At the same time, it can also compare and analyze multiple varieties, and dig out the unique genes and mutation sites of the variety. These mutation sites and unique genes are often related to the unique biological functions of the species. The pan-genome is sequenced at a higher level for different but interrelated individual materials and assembled separately to construct a pan-genome map in order to obtain complete genetic information of the species.
SERVICES
With years of rich experience, Lifeasible provides plant pan-genome sequencing service. We provide a one-stop service model. We complete a series of processes from sample extraction, quality testing, sequencing, biological information analysis to making reports.
Workflow of plant pan-genome sequencing and information analysis
According to the complexity of the species genome, corresponding to different sequencing indicators and assembly indicators, as shown below.
| Species genome characteristics | Simple genome | Complex genome | Hypercomplex genome | |||
|---|---|---|---|---|---|---|
| Assembly index | low | high | low | high | low | high |
| Sequencing strategy | 50×PacBio/Nanopore+100×Illumina | 80×PacBio/Nanopore+100×Illumina | 50×PacBio/Nanopore+100×Illumina | 100×PacBio/Nanopore+100×Illumina | 50×PacBio/Nanopore+100×Illumina | 100×PacBio/Nanopore+100×Illumina |
| Assembly index | contigN50＞500 Kb | contigN50＞2 Mb | contigN50＞100 Kb | contigN50＞500 Kb | contigN50＞100 Kb | contigN50＞100 Kb |
According to the nature of the material, the pan-genome mines the species specificity
- Materials of different subspecies
It can study important biological issues such as the origin and evolution of species.
- Wild species and cultivated species
It can discover genetic resources related to important traits and provide guidance for scientific Breeding.
- Different types of eco-geography (different lineages)
It can carry out research on popular scientific issues such as adaptive evolution of species and invasiveness of alien species, providing new research methods for Molecular ecology and other disciplines.
Project cycle
- The entire project generally takes about 2 months. The specific completion time depends on the specific scale of the project (number of samples and number of phenotype types).
Feedback to customers
- Raw data
- A project report includes experimental process, instrument parameters, analysis methods and results, etc.
Service process
ADVANTAGES
- From material selection, library construction and sequencing, to data analysis, every step has a scientific and meticulous design to ensure high-quality research results.
- Lifeasible has a high-performance computing platform (HPC) that uses an efficient combination of DELL computing nodes and isilon storage to achieve fast and stable sequencing data analysis and delivery.
- The continuously updated and expanded high-performance computing platform ensures efficient data processing and secure data storage.
- Personalized customized package service.
※ It should be noted that our service is only used for research.
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