Services
- Genetic Marker Assisted Breeding
- Plant Molecular Biology Research
- Research on DNA Level of Plant
- Research on Microspore Embryogenesis
- Plant Genetic Engineering
- Plant Genome Editing with CRISPR / Cas9
- Plant Genome Editing with TALEN
- Plant Genome Editing with ZFN
- RNAi Mediated Plant Gene Silencing
- Overexpression of Plant Genes
- Arabidopsis thaliana Transformation
- Oryza sativa Transformation
- Zea mays Transformation
- Triticum aestivum Transformation
- Medicago truncatula Transformation
- Glycine max Transformation
- Gossypium hirsutum Transformation
- Nicotiana tabacum Transformation
- Solanum lycopersicum Transformation
- Brassica napus Transformation
- Solanum tuberosum Transformation
- Virus-Induced Gene Silencing (VIGS) of Plant Genes
- Research on Protein Level of Plant
- Plant Epigenetic Modification Testing Services
- Sequencing-based Plant Breeding
- Plant DNA-level Sequencing Services
- Plant Genome De Novo Service
- Plant Whole Genome Resequencing Service
- Plant Reduced-Representation Genome Sequencing (RRGS)
- Plant Genetic Map Service
- BSA Trait Positioning of Plant
- Genome-Wide Association Study (GWAS) of Plant
- eQTL Analysis of Plant
- Plant Genetic Evolution Service
- Plant Pan-genome Sequencing
- Plant Whole Exome Sequencing Service
- Individual Selection Pressure Analysis of Plant
- Mixing-tank Selection Pressure Analysis of Plant
- Plant Whole Genome Survey
- Plant RNA Level Sequencing Services
- Eukaryotic Transcriptome Sequencing without Reference Genome
- Eukaryotic Transcriptome Sequencing with Reference Genome
- Prokaryotic Transcriptome Sequencing Analysis
- LncRNA Sequencing of Plant
- Plant Small RNA Sequencing
- Plant Circular RNA Sequencing
- Plant Comparative Transcriptome Service
- Plant Isoform-sequencing with Reference Genome
- Plant Isoform-sequencing without Reference Genome
- Ribo-seq of Plant
- Metatranscriptome Sequencing of Plant
- Plant Single Cell Level Sequencing Services
- Plant Epigenetics Level Sequencing Services
- Plant Proteomics Service
- Proteomics Qualitative Analysis in Plant
- Plant Protein Quantitative Analysis Service Based on Isotope Labeling (iTRAQ / TMT)
- Non-labeled Plant Protein Quantitative Analysis (Label-free / DIA)
- Plant Protein Targeted Quantitative Service (PRM / MRM / AQUA)
- Post-translational Modification Proteomics (PTMs) Service for Plant
- Plant Metabolomics Services
- Plant DNA-level Sequencing Services
- Other Services
- Plant CNV Analysis Service
- Plant Mutation Detection Service
- Plant Strain / Cell Level Services
- Plant Tissue and Cell Culture Services
- Plant Polyploidization Services
- Plant Haploidization Services
- Plant Phenotypic Analysis
- Plant Stress Response Indicators Analysis
- Plant Biochemical Analysis
- Plant Tissue and Cell Imaging Services
- Plant Disease Identification Services
- Plant Organelle Isolation Services
- Genetically Modified Plant Testing Services
- Seed Testing Services
Prokaryotic Transcriptome Sequencing Analysis
INQUIRYIntroductions
Prokaryotic transcriptome sequencing analysis is a transcriptomic study designed for prokaryotic microorganisms. Microorganisms will show differences in gene expression in specific habitats or periods in order to respond to environmental changes. After removing the ribosomal RNA, constructing a chain-specific library and other experimental steps, finally obtain the expression difference, structural information, and functional characteristics of all transcripts (including mRNA, non-coding RNA, etc.) in the microorganisms in a specific period or environment, so as to find out the key functional genes and solve more microbial function puzzles. This technology is mainly used to explore the molecular regulation mechanism of microorganisms in different periods, different phenotypes, and stress responses. Or used to improve microbial transcriptome information, such as transcript structure, new transcript prediction, non-coding RNA prediction, etc.
Services
With years of rich experience, Lifeasible provides prokaryotic transcriptome sequencing analysis service. We provide a one-stop service model. We complete a series of processes from sample extraction, quality testing, sequencing, biological information analysis to making reports.
Workflow of prokaryotic transcriptome sequencing analysis service
Analysis content
| Standard analysis | Sequencing data Quality control |
| Align reference genome | |
| Known gene expression statistics | |
| Sample relationship analysis | |
| Differential gene enrichment analysis (two or more samples) 1. Differentially expressed genes screening 2. Scatter plots and volcano plots of differential genes 3. Cluster analysis of differential gene expression patterns (heat map) 4. Significant enrichment analysis of GO functions of differential genes | |
| Novel transcript prediction | |
| Transcript structure analysis 1. Transcription start/stop site prediction 2. Operon prediction 3. Promoter prediction 4. 5'UTR ribosome binding region (SD) sequence prediction | |
| Antisense transcription Analysis | |
| sRNA analysis 1. sRNA prediction 2. sRNA annotation | |
| Circos diagram of gene structure | |
| Advanced analysis | sRNA target gene prediction and association analysis |
Sample requirements
- Total sample requirement≥4μg, concentration≥100ng/μl
- 1.8≤OD260/ 280≤2.2, OD260/230≥2.0, RIN≥6.5, 23S:16S≥1.0
- Please put the sample in a 1.5ml tube, indicate the sample name, concentration and preparation time on the tube, and use parafilm to seal the tube mouth.
- Before shipping, fix all sample tubes in 50ml centrifuge tubes with caps and ship them on dry ice.
Project cycle
- The entire project generally takes about 2 months.
Feedback to customers
- Raw data
- A project report includes experimental process, instrument parameters, analysis methods and results, etc.
Service process
Advantages
- From material selection, library construction and sequencing, to data analysis, every step has a scientific and meticulous design to ensure high-quality research results.
- The one-stop service we provide makes your upstream and downstream experiments seamlessly connected, facilitates your experiments more conveniently and quickly.
- The continuously updated and expanded high-performance computing platform ensures efficient data processing and secure data storage.
- Personalized customized package service.
- Standardization system, scientific management.
※ It should be noted that our service is only used for research.
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