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Breeding of Stress Resistant Alfalfa
INQUIRYIntroduction
Alfalfa is one of the legume forage grass with the largest planting area and enjoys the title of "king of forages". It not only has rich nutritional value and high yield, but also has developed root system, thick axial root, luxuriant branches, and strong drought resistance, cold resistance, salinity and alkalinity resistance and regeneration ability. Therefore, alfalfa plays an indispensable role in promoting the development of efficient and high-quality animal husbandry and ecological restoration. Alfalfa is mainly cultivated in saline-alkali land and poor soil, which can play a role in soil improvement. With the rapid development of molecular biology and plant genetic engineering, using biotechnology to improve the agronomic traits of alfalfa can breed new varieties with high quality and high yield, short breeding cycle and strong stress resistance, which is of great significance for the construction of cultivated grassland and the development of grassland animal husbandry.
Service
Improving the resistance of alfalfa to abiotic stresses is one of the breeding goals of alfalfa. With the development of biotechnology, more and more functional genes have been identified and discovered, which will provide a broader prospect for alfalfa anti-stress breeding. Lifeasible provides several strategies for accelerating the process of stress resistance breeding in alfalfa.
- Molecular Marker Technology
Molecular marker technology is an important means of biotechnology-assisted breeding. This technique overcomes the blindness of conventional breeding, speeds up the breeding process and improves the breeding efficiency. It can be used in alfalfa genetic diversity detection, construction of genetic linkage map, gene marker and mapping, transgenic mutation detection and many other aspects.
- Transgenic Genetic Engineering
Agrobacterium-mediated genetic transformation is the most commonly used genetic transformation method. CRISPR/Cas9 gene editing technology was used to modify the genes related to stress resistance in alfalfa, so as to obtain broad-spectrum and long-lasting stress resistance. As a model crop in modern gene research, alfalfa has a strong ability to regenerate and differentiate plants, and the transgenic technology has made great progress in its genetic improvement, drought resistance, salt resistance and quality improvement.
Figure 1. Responses of WT, p3302 and MtRAV3 transgenic groups in the pot salt treatment614 experiment. (Wang S.; et al. 2021)
- High-throughput Sequencing Technology
To understand the genetic mechanism of alfalfa resistance, Lifeasible offers a variety of technical services, including DNA-level sequencing, RNA-level sequencing, single-cell level sequencing, epigenetic-level sequencing, proteomics and metabolomics analysis.
Advantages
- Multiple advanced molecular biology platforms to provide different sources of gene transformation services to support your molecular breeding research
- Standardized management system and optimized experimental procedures to ensure efficient and accurate experimental results
- The professional technical team can customize the suitable genetic transformation program for you to meet the research needs of different customers and obtain the positive transformation seeds that can meet the downstream experiments
- Timely feedback and communication to ensure accurate implementation and repositioning of the agreement
- Experienced technical team, one-stop service, speed up the research process
As an expert in botany and agronomy, Lifeasible is committed to accelerating plant breeding research through molecular biology technology platforms. We will provide you with a one-stop experimental experience from the construction of the vector to the acquisition of transgenic seedlings and related physiological and biochemical tests. If you have related research needs or would like to know more about our services, please feel free to contact us.
Reference
- Wang, S.; et al. Overexpression of MtRAV3 enhances osmotic and salt tolerance and inhibits growth of Medicago truncatula. Plant Physiol Biochem. 2021, 163: 154-165.
※ It should be noted that our service is only used for research.
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