Plant Isozyme Activity Analysis Service

INQUIRY

Introductions

Biochemical markers mainly use gene expression products such as isoenzymes and storage proteins to reflect genotype differences to a certain extent. They are used in the genetic breeding of wheat, corn and other crops. Isozyme refers to a group of enzymes that catalyze the same chemical reaction, but have obvious differences in protein molecular structure, physical and chemical properties, and immune performance. This enzyme exists in the same genus of organisms, or in different tissues/cells of the same individual. The production of isoenzymes is due to the adaptation to metabolic processes during biological evolution. For enzymes that are more critical for metabolic regulation or closely related to the external environment, they also have isoenzyme properties and are more common, and at the same time there are more molecular types. Through the analysis of isoenzyme profiles, we can study a variety of information such as species evolution, genetic variation, hybrid breeding, and individual development differences. Therefore, this research is widely used in the field of plant molecular breeding.

Services

With years of rich experience, Lifeasible provides isozyme activity analysis service in plant. We provide a one-stop service model. As long as you provide experimental samples, we will complete a series of processes from sample extraction to data analysis.

Technical process

• • First, the samples were separated by SDS-polyacrylamide electrophoresis (SDS-PAGE, 0.1% gelatin).
• • Then the enzyme in the sample is restored to activity in a buffer system containing divalent metal ions.
  • The gelatin in the gel is hydrolyzed at the respective migration positions.
• • Finally, dye the gel with the corresponding dye and decolorize the gel.
  • The strength of the band is proportional to the enzyme activity.

Analysis content

We use electrophoresis analysis method to analyze the isozyme spectrum, and draw the isozyme spectrum based on the difference of electrophoretic mobility.

Sample requirements

• To ensure the reliability of the zymogram data, it is recommended to provide fresh tissue samples, or quick-freeze liquid nitrogen and send them on dry ice.

Project cycle

• There are many types of isoenzymes, and the detection methods and indicators of isoenzymes are quite different. Therefore, the detection period needs to be determined after preliminary experiments.

Feedback to customers

• A project report, including experimental procedures, experimental data (or pictures), analysis results, etc.

Service process

• Submit the relevant requirements and materials of the project.
• Discuss project details with our professional technical team.
• Revise the experimental plan based on the opinions after discussion.
• Both parties are satisfied and reach an agreement to sign a technical service contract.
• Carry out experiments and complete the content stipulated in the contract on time.
• After the project experiment is over, we will provide the original experimental data, analysis results, experimental procedures, experimental conditions, etc.

Advantages

• Lifeasible specializes in providing services related to agricultural molecular breeding, with standardized systems and scientific management.
• Advanced equipment, mature and stable methods.
• Optimized experimental procedures ensure extremely high efficiency and specificity.
• The one-stop service we provide makes your upstream and downstream experiments seamlessly connected, facilitates your experiments more conveniently and quickly.
• Personalized customized package service.

 

※ It should be noted that our service is only used for research.

Related Sections

• Plant Karyotype Analysis Service
• Plant STR / SSR Analysis Service
• Plant SNP Analysis Service

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