Services
- Genetic Marker Assisted Breeding
- Plant Molecular Biology Research
- Research on DNA Level of Plant
- Research on Microspore Embryogenesis
- Plant Genetic Engineering
- Plant Genome Editing with CRISPR / Cas9
- Plant Genome Editing with TALEN
- Plant Genome Editing with ZFN
- RNAi Mediated Plant Gene Silencing
- Overexpression of Plant Genes
- Arabidopsis thaliana Transformation
- Oryza sativa Transformation
- Zea mays Transformation
- Triticum aestivum Transformation
- Medicago truncatula Transformation
- Glycine max Transformation
- Gossypium hirsutum Transformation
- Nicotiana tabacum Transformation
- Solanum lycopersicum Transformation
- Brassica napus Transformation
- Solanum tuberosum Transformation
- Virus-Induced Gene Silencing (VIGS) of Plant Genes
- Research on Protein Level of Plant
- Plant Epigenetic Modification Testing Services
- Sequencing-based Plant Breeding
- Plant DNA-level Sequencing Services
- Plant Genome De Novo Service
- Plant Whole Genome Resequencing Service
- Plant Reduced-Representation Genome Sequencing (RRGS)
- Plant Genetic Map Service
- BSA Trait Positioning of Plant
- Genome-Wide Association Study (GWAS) of Plant
- eQTL Analysis of Plant
- Plant Genetic Evolution Service
- Plant Pan-genome Sequencing
- Plant Whole Exome Sequencing Service
- Individual Selection Pressure Analysis of Plant
- Mixing-tank Selection Pressure Analysis of Plant
- Plant Whole Genome Survey
- Plant RNA Level Sequencing Services
- Eukaryotic Transcriptome Sequencing without Reference Genome
- Eukaryotic Transcriptome Sequencing with Reference Genome
- Prokaryotic Transcriptome Sequencing Analysis
- LncRNA Sequencing of Plant
- Plant Small RNA Sequencing
- Plant Circular RNA Sequencing
- Plant Comparative Transcriptome Service
- Plant Isoform-sequencing with Reference Genome
- Plant Isoform-sequencing without Reference Genome
- Ribo-seq of Plant
- Metatranscriptome Sequencing of Plant
- Plant Single Cell Level Sequencing Services
- Plant Epigenetics Level Sequencing Services
- Plant Proteomics Service
- Proteomics Qualitative Analysis in Plant
- Plant Protein Quantitative Analysis Service Based on Isotope Labeling (iTRAQ / TMT)
- Non-labeled Plant Protein Quantitative Analysis (Label-free / DIA)
- Plant Protein Targeted Quantitative Service (PRM / MRM / AQUA)
- Post-translational Modification Proteomics (PTMs) Service for Plant
- Plant Metabolomics Services
- Plant DNA-level Sequencing Services
- Other Services
- Plant CNV Analysis Service
- Plant Mutation Detection Service
- Plant Strain / Cell Level Services
- Plant Tissue and Cell Culture Services
- Plant Polyploidization Services
- Plant Haploidization Services
- Plant Phenotypic Analysis
- Plant Stress Response Indicators Analysis
- Plant Biochemical Analysis
- Plant Tissue and Cell Imaging Services
- Plant Disease Identification Services
- Plant Organelle Isolation Services
- Genetically Modified Plant Testing Services
- Seed Testing Services
Plant Karyotype Analysis Service
INQUIRYIntroductions
Cytological markers refer to the variation of plant cell chromosomes, including chromosome karyotype (number of chromosomes, structure, satellite presence, centromere position, etc.) and band type (C-band, N-band, G-band, etc.) changes. Karyotype analysis refers to the process of artificially pairing, numbering and grouping the chromosomes, and performing morphological analysis of the cell to be tested according to the inherent chromosome morphological and structural characteristics of the organism. The chromosomes of different species have their own specific morphological and structural characteristics of chromosomes (including the number, length, centromere position, arm ratio, satellite size, etc.), and this morphological characteristic is relatively stable. After dyed or fluorescently labeled chromosomes, the specific morphology and structure of chromosomes can be clearly and intuitively observed through certain optical or electrochemical color development equipment, and then compared with normal karyotypes to find differences, and determine chromosome deletions, duplications, inversions, and other phenomena. As a basic method of cytogenetics research, chromosome karyotype analysis is an important method to study species evolution, classification and the relationship between chromosome structure, morphology and function. Especially it has important application value for plant genetics and classification.
Services
With years of rich experience, Lifeasible provides plant karyotype analysis service. We provide a one-stop service model. We complete a series of processes from sample recovery, cell culture, making microscope specimens, karyotype analysis to making reports.
Technical process
Analysis content
| Morphological analysis | Select the chromosomes from the metaphase of mitosis of vigorously dividing cells to make a karyotype map, determine the length (micrometer) or relative length (%) of each chromosome, determine the centromere position and the arm length ratio of the chromosome, and identify satellites and constrictions. |
| Band analysis | G-banding technology is to color different areas of chromosomes by Giemsa staining method, thereby showing light and dark bands under a light microscope. Each chromosome has a specific band pattern, and even the long and short arms of each chromosome are specific. According to the different banding types of chromosomes, the individuality of chromosomes can be identified more carefully and accurately. |
| Colored segment analysis | After the chromosomes are processed, a heteropyknosis reaction occurs, which makes the heterochromatin segments visible. The coloring segments are basically the same between homologous chromosomes, but there are differences between non-homologous chromosomes. |
Sample requirements
- Cell suspension
- Cell cryopreservation tube
Project cycle
- The entire project generally takes about 7 working days.
Feedback to customers
- A project report, including experimental procedures, experimental data, analysis results, etc.
Service process
Advantages
- Lifeasible specializes in providing services related to agricultural molecular breeding, with standardized systems and scientific management.
- Advanced equipment, mature and stable methods.
- Optimized experimental procedures ensure extremely high efficiency and specificity.
- The one-stop service we provide makes your upstream and downstream experiments seamlessly connected, facilitates your experiments more conveniently and quickly.
- Personalized customized package service.
※ For research or industrial use.
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