Services

Plant Karyotype Analysis Service

INQUIRY

Introductions

Genetic Marker Assisted Breeding

Cytological markers refer to the variation of plant cell chromosomes, including chromosome karyotype (number of chromosomes, structure, satellite presence, centromere position, etc.) and band type (C-band, N-band, G-band, etc.) changes. Karyotype analysis refers to the process of artificially pairing, numbering and grouping the chromosomes, and performing morphological analysis of the cell to be tested according to the inherent chromosome morphological and structural characteristics of the organism. The chromosomes of different species have their own specific morphological and structural characteristics of chromosomes (including the number, length, centromere position, arm ratio, satellite size, etc.), and this morphological characteristic is relatively stable. After dyed or fluorescently labeled chromosomes, the specific morphology and structure of chromosomes can be clearly and intuitively observed through certain optical or electrochemical color development equipment, and then compared with normal karyotypes to find differences, and determine chromosome deletions, duplications, inversions, and other phenomena. As a basic method of cytogenetics research, chromosome karyotype analysis is an important method to study species evolution, classification and the relationship between chromosome structure, morphology and function. Especially it has important application value for plant genetics and classification.

Services

With years of rich experience, Lifeasible provides plant karyotype analysis service. We provide a one-stop service model. We complete a series of processes from sample recovery, cell culture, making microscope specimens, karyotype analysis to making reports.

Technical process

Technical process

Analysis content

Morphological analysis Select the chromosomes from the metaphase of mitosis of vigorously dividing cells to make a karyotype map, determine the length (micrometer) or relative length (%) of each chromosome, determine the centromere position and the arm length ratio of the chromosome, and identify satellites and constrictions.
Band analysis G-banding technology is to color different areas of chromosomes by Giemsa staining method, thereby showing light and dark bands under a light microscope. Each chromosome has a specific band pattern, and even the long and short arms of each chromosome are specific. According to the different banding types of chromosomes, the individuality of chromosomes can be identified more carefully and accurately.
Colored segment analysis After the chromosomes are processed, a heteropyknosis reaction occurs, which makes the heterochromatin segments visible. The coloring segments are basically the same between homologous chromosomes, but there are differences between non-homologous chromosomes.

Sample requirements

  • Cell suspension
  • Cell cryopreservation tube

Project cycle

  • The entire project generally takes about 7 working days.

Feedback to customers

  • A project report, including experimental procedures, experimental data, analysis results, etc.

Service process

Plant Karyotype Analysis Service Process -Lifeasible

Advantages

  • Lifeasible specializes in providing services related to agricultural molecular breeding, with standardized systems and scientific management.
  • Advanced equipment, mature and stable methods.
  • Optimized experimental procedures ensure extremely high efficiency and specificity.
  • The one-stop service we provide makes your upstream and downstream experiments seamlessly connected, facilitates your experiments more conveniently and quickly.
  • Personalized customized package service.

※ It should be noted that our service is only used for research.

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