Services
- Genetic Marker Assisted Breeding
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- Research on DNA Level of Plant
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- Overexpression of Plant Genes
- Arabidopsis thaliana Transformation
- Oryza sativa Transformation
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- Virus-Induced Gene Silencing (VIGS) of Plant Genes
- Research on Protein Level of Plant
- Plant Epigenetic Modification Testing Services
- Sequencing-based Plant Breeding
- Plant DNA-level Sequencing Services
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- Plant Whole Genome Resequencing Service
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- Plant Genetic Map Service
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- Genome-Wide Association Study (GWAS) of Plant
- eQTL Analysis of Plant
- Plant Genetic Evolution Service
- Plant Pan-genome Sequencing
- Plant Whole Exome Sequencing Service
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- Mixing-tank Selection Pressure Analysis of Plant
- Plant Whole Genome Survey
- Plant RNA Level Sequencing Services
- Eukaryotic Transcriptome Sequencing without Reference Genome
- Eukaryotic Transcriptome Sequencing with Reference Genome
- Prokaryotic Transcriptome Sequencing Analysis
- LncRNA Sequencing of Plant
- Plant Small RNA Sequencing
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- Plant Comparative Transcriptome Service
- Plant Isoform-sequencing with Reference Genome
- Plant Isoform-sequencing without Reference Genome
- Ribo-seq of Plant
- Metatranscriptome Sequencing of Plant
- Plant Single Cell Level Sequencing Services
- Plant Epigenetics Level Sequencing Services
- Plant Proteomics Service
- Proteomics Qualitative Analysis in Plant
- Plant Protein Quantitative Analysis Service Based on Isotope Labeling (iTRAQ / TMT)
- Non-labeled Plant Protein Quantitative Analysis (Label-free / DIA)
- Plant Protein Targeted Quantitative Service (PRM / MRM / AQUA)
- Post-translational Modification Proteomics (PTMs) Service for Plant
- Plant Metabolomics Services
- Plant DNA-level Sequencing Services
- Other Services
- Plant CNV Analysis Service
- Plant Mutation Detection Service
- Plant Strain / Cell Level Services
- Plant Tissue and Cell Culture Services
- Plant Polyploidization Services
- Plant Haploidization Services
- Plant Phenotypic Analysis
- Plant Stress Response Indicators Analysis
- Plant Biochemical Analysis
- Plant Tissue and Cell Imaging Services
- Plant Disease Identification Services
- Plant Organelle Isolation Services
- Genetically Modified Plant Testing Services
- Seed Testing Services
Lipidomics Service in Plant
INQUIRYIntroductions
Lipids are a large class of compounds that are easily soluble in organic solvents and are non-uniform in chemical composition and structure. It mainly includes the following eight categories (named in the LIPID MAPS system): fatty acids (such as linoleic acid, arachidic acid), glycerolipids (such as TG, DG), glycerophospholipids (such as PC, PE, PG, PA, etc.), sphingolipids (such as Cer, SM, etc.), sterol lipids (such as sterol lipids), glycolipids (such as MGDG, SQDG, etc.), pregnenolone lipids (such as CoA, etc.), polyketides. Lipidomics is a branch of metabolomics, which is mainly based on the high-throughput LC-MS/MS technology platform to systematically study the composition and expression changes of lipids in organisms. Then clarify the biological activity mechanism and principle of lipid family or lipid molecules, and the discipline of their role in protein expression and gene regulation. Lipidomics also plays a very important role in plant molecular breeding. For example, it is used as an auxiliary method to study the biological or abiotic stress of plants, the study of growth and development, and the study of crop nutritional quality.
Services
Workflow of plant lipidomics service
Sample requirements
- Sample type
- Sample mix
- Sample weight and repeat
- Storage and transportation
Cells with medium removed, leaves, stems, flowers, etc.
If the separation conditions are not available in the field, the pulp and peel of the fruit should be transferred to the room as soon as possible (within 30 minutes), and the separated sample should be placed in liquid nitrogen, and then stored at -80°C.
Each sample is mixed from at least 3 samples with the same conditions. For example, we need to collect samples from three plants with the same phenotype at the same time and at the same location, and then mix them.
For fresh and clean plant samples, it is recommended that each sample is ≥ 200 mg, and prepare 6 or more biological replicates.
After collecting the sample, put it in liquid nitrogen immediately and store it at -80°C.
Dry ice transportation (about 3~4 kg of dry ice is consumed every day, please use sufficient dry ice for transportation).
Project cycle
- It takes 60~90 working days to complete the standard process.
Feedback to customers
- Raw data
- A project report includes experimental procedures, related mass spectrometry parameters, partial mass spectrometry pictures, raw data, and metabolomics analysis results
Service process
Advantages
- Lifeasible specializes in providing services related to agricultural molecular breeding, with standardized systems and scientific management.
- The experiment adopts a carefully optimized experimental procedure, which has extremely high efficiency and specificity.
- The one-stop service we provide makes your upstream and downstream experiments seamlessly connected, facilitates your experiments more conveniently and quickly.
- With a strong bioinformatics team, it can meet the various in-depth data analysis needs of customers.
- Personalized customized package service.
※ It should be noted that our service is only used for research.
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