Services
- Genetic Marker Assisted Breeding
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- Research on DNA Level of Plant
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- Overexpression of Plant Genes
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- Nicotiana tabacum Transformation
- Solanum lycopersicum Transformation
- Brassica napus Transformation
- Solanum tuberosum Transformation
- Virus-Induced Gene Silencing (VIGS) of Plant Genes
- Research on Protein Level of Plant
- Plant Epigenetic Modification Testing Services
- Sequencing-based Plant Breeding
- Plant DNA-level Sequencing Services
- Plant Genome De Novo Service
- Plant Whole Genome Resequencing Service
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- Plant Pan-genome Sequencing
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- Eukaryotic Transcriptome Sequencing without Reference Genome
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- LncRNA Sequencing of Plant
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- Plant Comparative Transcriptome Service
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- Plant Isoform-sequencing without Reference Genome
- Ribo-seq of Plant
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- Plant Single Cell Level Sequencing Services
- Plant Epigenetics Level Sequencing Services
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- Proteomics Qualitative Analysis in Plant
- Plant Protein Quantitative Analysis Service Based on Isotope Labeling (iTRAQ / TMT)
- Non-labeled Plant Protein Quantitative Analysis (Label-free / DIA)
- Plant Protein Targeted Quantitative Service (PRM / MRM / AQUA)
- Post-translational Modification Proteomics (PTMs) Service for Plant
- Plant Metabolomics Services
- Plant DNA-level Sequencing Services
- Other Services
- Plant CNV Analysis Service
- Plant Mutation Detection Service
- Plant Strain / Cell Level Services
- Plant Tissue and Cell Culture Services
- Plant Polyploidization Services
- Plant Haploidization Services
- Plant Phenotypic Analysis
- Plant Stress Response Indicators Analysis
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- Plant Tissue and Cell Imaging Services
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- Plant Organelle Isolation Services
- Genetically Modified Plant Testing Services
- Seed Testing Services
Nicotiana tabacum Transformation
INQUIRYIntroductions
Tobacco, whose scientific name is Nicotiana tabacum L., is an annual or limited perennial herb of the solanaceae and nicotiana genus. It originated in tropical and subtropical America. It is a rich source of the alkaloid nicotine and has long been used as a herbal medicine and stimulant. So far, tobacco has been grown worldwide. It is not only a major cash crop, but also a model plant for studying plant genetics, molecular biology and cell biology. Because tobacco is easy to tissue culture and has high gene transformation efficiency, tobacco is an important model plant in plant genetic engineering and molecular biology research.
Services
Lifeasible has established a tobacco genetic transformation platform with a multi-temperature greenhouse system and a mature seedling system. Use mature tobacco varieties to provide large-scale, standardized genetic modification technology services. Tobacco genetic transformation adopts Agrobacterium-mediated leaf disc infection method, gene gun bombardment method and PEG-mediated transformation method, with high infection efficiency, and enough positive seedlings can be obtained in one transformation, save time and cost for scientific research workers.
Customer provided materials
- If only gene sequence information (or Gene Bank ID) is provided, our company can undertake gene amplification and vector construction services, which are charged separately.
- Construct a vector of Escherichia coli, plasmid or Agrobacterium, but provide clear resistance information for screening.
- Unconventional receptor material
※ We do not accept the transformation service of lethal genes or severely defective genes. If there are any of the above, please inform our company in advance.
Nicotiana tabacum transformation methods
- Agrobacterium-mediated transformation
- Biolistic particle delivery system
- Polyethylene glycol (PEG)-mediated transformation of protoplasts
Receptor material
- Recipient species
- Recipient types
NB, Xanthi, K326, yun87, CB-1, SR1, W38, G28, AK16 and other varieties.
Agrobacterium-mediated transformation - leaf disc
Biolistic particle delivery system - explant
PEG-mediated transformation - protoplasts
Workflow of tobacco transformation by Agrobacterium-mediated method
Agrobacterium-mediated transient transformation of tobacco by leaf microinjection (Stephen Millam, 2013).
Feedback to customers
- Complete experimental methods, original experimental records, instrument parameters, reagents and consumables information.
- More than 10 independent positive lines.
- PCR verification of genome of transgenic plants.
- Pictures of explant infection, induced callus, embryogenic callus, tissue differentiation, plant regeneration, etc.
Service process
Project cycle
- It takes about 4 months.
Advantages
- It adopts Agrobacterium-mediated transformation, which is fast, efficient, stable, single-copy insertion, and is not limited by genotype.
- For commonly used tobacco varieties, sufficient sterile seedlings are prepared all year round, and transformation experiments can be carried out at any time to save customers' time.
- We have a large-scale, standardized seedling system to ensure the success rate of experimental transformation.
- We have a variety of labeled vectors and media to provide you with a wide range of choices.
- Personalized customized package service.
Reference
- Stephen Millam. Plant Tissue Culture. Techniques and Experiments [J]. Experimental Agriculture, 2013, 49(2).
※ It should be noted that our service is only used for research.
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