Services
- Genetic Marker Assisted Breeding
- Plant Molecular Biology Research
- Research on DNA Level of Plant
- Research on Microspore Embryogenesis
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- Overexpression of Plant Genes
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- Plant Epigenetic Modification Testing Services
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- Plant DNA-level Sequencing Services
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- Eukaryotic Transcriptome Sequencing without Reference Genome
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- Ribo-seq of Plant
- Metatranscriptome Sequencing of Plant
- Plant Single Cell Level Sequencing Services
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- Proteomics Qualitative Analysis in Plant
- Plant Protein Quantitative Analysis Service Based on Isotope Labeling (iTRAQ / TMT)
- Non-labeled Plant Protein Quantitative Analysis (Label-free / DIA)
- Plant Protein Targeted Quantitative Service (PRM / MRM / AQUA)
- Post-translational Modification Proteomics (PTMs) Service for Plant
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- Plant DNA-level Sequencing Services
- Other Services
- Plant CNV Analysis Service
- Plant Mutation Detection Service
- Plant Strain / Cell Level Services
- Plant Tissue and Cell Culture Services
- Plant Polyploidization Services
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- Plant Phenotypic Analysis
- Plant Stress Response Indicators Analysis
- Plant Biochemical Analysis
- Plant Tissue and Cell Imaging Services
- Plant Disease Identification Services
- Plant Organelle Isolation Services
- Genetically Modified Plant Testing Services
- Seed Testing Services
MeDIP-seq in Plant
INQUIRYIntroductions
MeDIP-seq (Methylated DNA Immunoprecipitation Sequencing) and is a whole genome methylation detection technology based on the principle of antibody enrichment for sequencing, It uses methylated DNA immunoprecipitation technology to specifically enrich the methylated DNA fragments on the genome through 5'-methylcytosine (5mC) antibody, and then can be performed at the whole genome level through high-throughput sequencing and high-precision study of densely methylated CpG regions. hMeDIP-seq (hydroxymethylcytosine DNA Immunoprecipitation Sequencing) is based on the same principle as MeDIP-Seq. The 5'-hydroxymethylcytosine (5hmC) antibody is used to specifically enrich the DNA fragments that are methylated on the genome. These two technologies have broad application prospects in large-scale plant breeding sample research. Researchers can use the MeDIP-Seq and hMeDIP-Seq technologies to quickly and effectively find methylated regions on the genome, so as to compare the differences in DNA methylation modification patterns among samples of different cells, tissues or different levels of stress environment. The academic research program provides theoretical evidence.
Services
With years of rich experience, Lifeasible provides methylated DNA immunoprecipitation sequencing (MeDIP-seq) and analysis services. Customers only need to provide tissues or DNA sample, and we complete the entire service process from sample extraction, quality testing, sequencing, biological information analysis to making reports.
Workflow of MeDIP-seq in plant
Analysis content
- Quality assessment of sequencing data
- Comparison of enriched sequence with reference sequence
- Overview of methylation information
- Peak calling
- Peak distribution in genes and intergenic regions
- Peak enrichment statistics in genomic regions
- Peak area function annotation and enrichment analysis
Sample requirements
- DNA ≥ 3µg, concentration ≥ 50ng/µl, OD 260/280=1.8~2.0, no RNA pollution, no degradation.
- The sample should be dissolved in sterile 10 mM TirsHCl, 1 mM EDTAE (pH 8.0) solution.
- DNA is transported at low temperature (-20°C), and please seal it with parafilm during transport to prevent contamination.
Project cycle
- It takes 60~90 working days to complete the standard process.
Feedback to customers
- Raw data
- A project report includes experimental process, instrument parameters, analysis methods and results, etc.
Service process
Advantages
- Lifeasible specializes in providing services related to agricultural molecular breeding, with standardized systems and scientific management.
- The experiment adopts a carefully optimized experimental procedure, which has extremely high efficiency and specificity.
- With a strong bioinformatics team, it can meet the various in-depth data analysis needs of customers.
- The one-stop service we provide makes your upstream and downstream experiments seamlessly connected, facilitates your experiments more conveniently and quickly.
- Personalized customized package service.
※ For research or industrial use.
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