Services
- Genetic Marker Assisted Breeding
- Plant Molecular Biology Research
- Research on DNA Level of Plant
- Research on Microspore Embryogenesis
- Plant Genetic Engineering
- Plant Genome Editing with CRISPR / Cas9
- Plant Genome Editing with TALEN
- Plant Genome Editing with ZFN
- RNAi Mediated Plant Gene Silencing
- Overexpression of Plant Genes
- Arabidopsis thaliana Transformation
- Oryza sativa Transformation
- Zea mays Transformation
- Triticum aestivum Transformation
- Medicago truncatula Transformation
- Glycine max Transformation
- Gossypium hirsutum Transformation
- Nicotiana tabacum Transformation
- Solanum lycopersicum Transformation
- Brassica napus Transformation
- Solanum tuberosum Transformation
- Virus-Induced Gene Silencing (VIGS) of Plant Genes
- Research on Protein Level of Plant
- Plant Epigenetic Modification Testing Services
- Sequencing-based Plant Breeding
- Plant DNA-level Sequencing Services
- Plant Genome De Novo Service
- Plant Whole Genome Resequencing Service
- Plant Reduced-Representation Genome Sequencing (RRGS)
- Plant Genetic Map Service
- BSA Trait Positioning of Plant
- Genome-Wide Association Study (GWAS) of Plant
- eQTL Analysis of Plant
- Plant Genetic Evolution Service
- Plant Pan-genome Sequencing
- Plant Whole Exome Sequencing Service
- Individual Selection Pressure Analysis of Plant
- Mixing-tank Selection Pressure Analysis of Plant
- Plant Whole Genome Survey
- Plant RNA Level Sequencing Services
- Eukaryotic Transcriptome Sequencing without Reference Genome
- Eukaryotic Transcriptome Sequencing with Reference Genome
- Prokaryotic Transcriptome Sequencing Analysis
- LncRNA Sequencing of Plant
- Plant Small RNA Sequencing
- Plant Circular RNA Sequencing
- Plant Comparative Transcriptome Service
- Plant Isoform-sequencing with Reference Genome
- Plant Isoform-sequencing without Reference Genome
- Ribo-seq of Plant
- Metatranscriptome Sequencing of Plant
- Plant Single Cell Level Sequencing Services
- Plant Epigenetics Level Sequencing Services
- Plant Proteomics Service
- Proteomics Qualitative Analysis in Plant
- Plant Protein Quantitative Analysis Service Based on Isotope Labeling (iTRAQ / TMT)
- Non-labeled Plant Protein Quantitative Analysis (Label-free / DIA)
- Plant Protein Targeted Quantitative Service (PRM / MRM / AQUA)
- Post-translational Modification Proteomics (PTMs) Service for Plant
- Plant Metabolomics Services
- Plant DNA-level Sequencing Services
- Other Services
- Plant CNV Analysis Service
- Plant Mutation Detection Service
- Plant Strain / Cell Level Services
- Plant Tissue and Cell Culture Services
- Plant Polyploidization Services
- Plant Haploidization Services
- Plant Phenotypic Analysis
- Plant Stress Response Indicators Analysis
- Plant Biochemical Analysis
- Plant Tissue and Cell Imaging Services
- Plant Disease Identification Services
- Plant Organelle Isolation Services
- Genetically Modified Plant Testing Services
- Seed Testing Services
MeDIP-seq in Plant
INQUIRYIntroductions
MeDIP-seq (Methylated DNA Immunoprecipitation Sequencing) and is a whole genome methylation detection technology based on the principle of antibody enrichment for sequencing, It uses methylated DNA immunoprecipitation technology to specifically enrich the methylated DNA fragments on the genome through 5'-methylcytosine (5mC) antibody, and then can be performed at the whole genome level through high-throughput sequencing and high-precision study of densely methylated CpG regions. hMeDIP-seq (hydroxymethylcytosine DNA Immunoprecipitation Sequencing) is based on the same principle as MeDIP-Seq. The 5'-hydroxymethylcytosine (5hmC) antibody is used to specifically enrich the DNA fragments that are methylated on the genome. These two technologies have broad application prospects in large-scale plant breeding sample research. Researchers can use the MeDIP-Seq and hMeDIP-Seq technologies to quickly and effectively find methylated regions on the genome, so as to compare the differences in DNA methylation modification patterns among samples of different cells, tissues or different levels of stress environment. The academic research program provides theoretical evidence.
Services
With years of rich experience, Lifeasible provides methylated DNA immunoprecipitation sequencing (MeDIP-seq) and analysis services. Customers only need to provide tissues or DNA sample, and we complete the entire service process from sample extraction, quality testing, sequencing, biological information analysis to making reports.
Workflow of MeDIP-seq in plant
Analysis content
- Quality assessment of sequencing data
- Comparison of enriched sequence with reference sequence
- Overview of methylation information
- Peak calling
- Peak distribution in genes and intergenic regions
- Peak enrichment statistics in genomic regions
- Peak area function annotation and enrichment analysis
Sample requirements
- DNA ≥ 3µg, concentration ≥ 50ng/µl, OD 260/280=1.8~2.0, no RNA pollution, no degradation.
- The sample should be dissolved in sterile 10 mM TirsHCl, 1 mM EDTAE (pH 8.0) solution.
- DNA is transported at low temperature (-20°C), and please seal it with parafilm during transport to prevent contamination.
Project cycle
- It takes 60~90 working days to complete the standard process.
Feedback to customers
- Raw data
- A project report includes experimental process, instrument parameters, analysis methods and results, etc.
Service process
Advantages
- Lifeasible specializes in providing services related to agricultural molecular breeding, with standardized systems and scientific management.
- The experiment adopts a carefully optimized experimental procedure, which has extremely high efficiency and specificity.
- With a strong bioinformatics team, it can meet the various in-depth data analysis needs of customers.
- The one-stop service we provide makes your upstream and downstream experiments seamlessly connected, facilitates your experiments more conveniently and quickly.
- Personalized customized package service.
※ It should be noted that our service is only used for research.
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