Services
- Genetic Marker Assisted Breeding
- Plant Molecular Biology Research
- Research on DNA Level of Plant
- Research on Microspore Embryogenesis
- Plant Genetic Engineering
- Plant Genome Editing with CRISPR / Cas9
- Plant Genome Editing with TALEN
- Plant Genome Editing with ZFN
- RNAi Mediated Plant Gene Silencing
- Overexpression of Plant Genes
- Arabidopsis thaliana Transformation
- Oryza sativa Transformation
- Zea mays Transformation
- Triticum aestivum Transformation
- Medicago truncatula Transformation
- Glycine max Transformation
- Gossypium hirsutum Transformation
- Nicotiana tabacum Transformation
- Solanum lycopersicum Transformation
- Brassica napus Transformation
- Solanum tuberosum Transformation
- Virus-Induced Gene Silencing (VIGS) of Plant Genes
- Research on Protein Level of Plant
- Plant Epigenetic Modification Testing Services
- Sequencing-based Plant Breeding
- Plant DNA-level Sequencing Services
- Plant Genome De Novo Service
- Plant Whole Genome Resequencing Service
- Plant Reduced-Representation Genome Sequencing (RRGS)
- Plant Genetic Map Service
- BSA Trait Positioning of Plant
- Genome-Wide Association Study (GWAS) of Plant
- eQTL Analysis of Plant
- Plant Genetic Evolution Service
- Plant Pan-genome Sequencing
- Plant Whole Exome Sequencing Service
- Individual Selection Pressure Analysis of Plant
- Mixing-tank Selection Pressure Analysis of Plant
- Plant Whole Genome Survey
- Plant RNA Level Sequencing Services
- Eukaryotic Transcriptome Sequencing without Reference Genome
- Eukaryotic Transcriptome Sequencing with Reference Genome
- Prokaryotic Transcriptome Sequencing Analysis
- LncRNA Sequencing of Plant
- Plant Small RNA Sequencing
- Plant Circular RNA Sequencing
- Plant Comparative Transcriptome Service
- Plant Isoform-sequencing with Reference Genome
- Plant Isoform-sequencing without Reference Genome
- Ribo-seq of Plant
- Metatranscriptome Sequencing of Plant
- Plant Single Cell Level Sequencing Services
- Plant Epigenetics Level Sequencing Services
- Plant Proteomics Service
- Proteomics Qualitative Analysis in Plant
- Plant Protein Quantitative Analysis Service Based on Isotope Labeling (iTRAQ / TMT)
- Non-labeled Plant Protein Quantitative Analysis (Label-free / DIA)
- Plant Protein Targeted Quantitative Service (PRM / MRM / AQUA)
- Post-translational Modification Proteomics (PTMs) Service for Plant
- Plant Metabolomics Services
- Plant DNA-level Sequencing Services
- Other Services
- Plant CNV Analysis Service
- Plant Mutation Detection Service
- Plant Strain / Cell Level Services
- Plant Tissue and Cell Culture Services
- Plant Polyploidization Services
- Plant Haploidization Services
- Plant Phenotypic Analysis
- Plant Stress Response Indicators Analysis
- Plant Biochemical Analysis
- Plant Tissue and Cell Imaging Services
- Plant Disease Identification Services
- Plant Organelle Isolation Services
- Genetically Modified Plant Testing Services
- Seed Testing Services
Zea mays Transformation
INQUIRYIntroductions
Corn is an annual herb of the Gramineae and Zea genus, with the Latin name Zea mays (L.). Maize is a monoecious cross-pollinated plant with tall plants and strong stalks. It is an important food crop and fodder crop, and has high nutritional and economic value. However, due to its relatively complex heritability and rich varieties of variation, there are shortcomings in the conventional Breeding process such as too long cycle, too large coefficient of variation, and affecting the growth and development of offspring. Modern biological breeding technology not only overcomes the above shortcomings, but also improved breeding speed and quality. With the development and application of genetic modification technology in modern agriculture, people have bred genetically modified corn with herbicide resistance, insect resistance, drought tolerance, and high carbohydrate content.
Services
Lifeasible has established a Zea mays transformation platform through Agrobacterium-mediated method and biolistic-mediated method. The platform has a complete, large-scale, standardized technical system, and provides professional and convenient technologies for maize genetic transformation for agricultural personnel who serve maize as the research object.
Customer provided materials
- The sequence of the target gene and the Gene Bank ID
- Plasmids, Agrobacterium, etc.
- Vector to be transformed (conventional vector can also be provided by us)
- Unconventional receptor material
※ We do not accept the transformation service of lethal genes or severely defective genes. If there are any of the above, please inform our company in advance.
Receptor material
- Recipient material varieties
- Recipient material types: immature embryo, mature embryo
Inbred lines: A188, A634, H99, W117, Y423, PHR03, Mo17, B104, B114, Ky21, etc.
Hybrid line: Hi-II
Workflow of maize transformation
- Vector construction
- Induction and subculture of Maize recipient callus
- Agrobacterium-mediated or biolistic-mediated of maize genetic transformation
- Screening
- Differentiation of positive callus into shoots
- Strong plantlets and rootage of regenerated green seedlings
- PCR detection of foreign genes in T0 generation transgenic seedlings
Feedback to customers
- Complete experimental methods, original experimental records, instrument parameters, reagents and consumables information
- Some pictures of genetic transformation and screening
- Picture of some samples of callus differentiation and regeneration
- Agarose gel electrophoresis image of foreign gene PCR detection of T0 generation transgenic seedlings
- T0 generation transgenic vaccine: If we use the vector and recipient provided by us, the T0 generation positive vaccine for each transformation experiment is ≥12; if the customer needs to use other vectors and recipients, we usually only promise to get the T0 generation positive vaccine 6-8 strains.
Service process
Project cycle
It takes 5-6 months from the construction of the vector to the test results of the T0 generation transgenic seedlings. And experiments can be carried out throughout the year.
Advantages
- We provide two systems for your choice: Agrobacterium-mediated method and gene gun bombardment method.
- We provide conventional receptor materials and vectors for your choice.
- We have an experienced team to guide you in designing and implementing experiments.
- The positive rate of genetically modified corn is higher.
- Leading research capabilities to meet your customized requirements.
- With excellent innovation ability, we can design novel solutions to adapt to the frontier development trend of the industry.
※ It should be noted that our service is only used for research.
Online Inquiry
