Services
- Genetic Marker Assisted Breeding
- Plant Molecular Biology Research
- Research on DNA Level of Plant
- Research on Microspore Embryogenesis
- Plant Genetic Engineering
- Plant Genome Editing with CRISPR / Cas9
- Plant Genome Editing with TALEN
- Plant Genome Editing with ZFN
- RNAi Mediated Plant Gene Silencing
- Overexpression of Plant Genes
- Arabidopsis thaliana Transformation
- Oryza sativa Transformation
- Zea mays Transformation
- Triticum aestivum Transformation
- Medicago truncatula Transformation
- Glycine max Transformation
- Gossypium hirsutum Transformation
- Nicotiana tabacum Transformation
- Solanum lycopersicum Transformation
- Brassica napus Transformation
- Solanum tuberosum Transformation
- Virus-Induced Gene Silencing (VIGS) of Plant Genes
- Research on Protein Level of Plant
- Plant Epigenetic Modification Testing Services
- Sequencing-based Plant Breeding
- Plant DNA-level Sequencing Services
- Plant Genome De Novo Service
- Plant Whole Genome Resequencing Service
- Plant Reduced-Representation Genome Sequencing (RRGS)
- Plant Genetic Map Service
- BSA Trait Positioning of Plant
- Genome-Wide Association Study (GWAS) of Plant
- eQTL Analysis of Plant
- Plant Genetic Evolution Service
- Plant Pan-genome Sequencing
- Plant Whole Exome Sequencing Service
- Individual Selection Pressure Analysis of Plant
- Mixing-tank Selection Pressure Analysis of Plant
- Plant Whole Genome Survey
- Plant RNA Level Sequencing Services
- Eukaryotic Transcriptome Sequencing without Reference Genome
- Eukaryotic Transcriptome Sequencing with Reference Genome
- Prokaryotic Transcriptome Sequencing Analysis
- LncRNA Sequencing of Plant
- Plant Small RNA Sequencing
- Plant Circular RNA Sequencing
- Plant Comparative Transcriptome Service
- Plant Isoform-sequencing with Reference Genome
- Plant Isoform-sequencing without Reference Genome
- Ribo-seq of Plant
- Metatranscriptome Sequencing of Plant
- Plant Single Cell Level Sequencing Services
- Plant Epigenetics Level Sequencing Services
- Plant Proteomics Service
- Proteomics Qualitative Analysis in Plant
- Plant Protein Quantitative Analysis Service Based on Isotope Labeling (iTRAQ / TMT)
- Non-labeled Plant Protein Quantitative Analysis (Label-free / DIA)
- Plant Protein Targeted Quantitative Service (PRM / MRM / AQUA)
- Post-translational Modification Proteomics (PTMs) Service for Plant
- Plant Metabolomics Services
- Plant DNA-level Sequencing Services
- Other Services
- Plant CNV Analysis Service
- Plant Mutation Detection Service
- Plant Strain / Cell Level Services
- Plant Tissue and Cell Culture Services
- Plant Polyploidization Services
- Plant Haploidization Services
- Plant Phenotypic Analysis
- Plant Stress Response Indicators Analysis
- Plant Biochemical Analysis
- Plant Tissue and Cell Imaging Services
- Plant Disease Identification Services
- Plant Organelle Isolation Services
- Genetically Modified Plant Testing Services
- Seed Testing Services
Virus-Induced Gene Silencing (VIGS) of Plant Genes
INQUIRYIntroductions
Virus induced gene silencing (VIGS) is a new technology suitable for rapid and high-throughput research and identification of plant gene functions. Its principle is to use the natural defense mechanism of plants against viruses. When the endogenous target gene of the plant is loaded into the plant virus vector and the plant is inoculated, the virus infects the plant systemically, spreads to the whole plant, and simultaneously expresses the double-stranded RNA (dsRNA) of the target gene. This dsRNA will trigger the RNAi mechanism in plants to degrade the endogenous transcription of the target gene in the plant to produce mRNA, resulting in the down-regulation or inhibition of the function of the target gene, so as to understand the function of the target gene through combined analysis with plant phenotypic changes.
Services
Lifeasible has established plant VIGS technology platform, used to serve research workers who need VIGS to assist research. Once the VIGS technology system is established, it is regarded as a powerful tool for studying plant gene functions, and has been in-depth research and widely used. It has been used in tobacco, tomato and other plants to study the functional genes of disease resistance, growth and development and metabolic regulation. We uphold the customer-centric tenet and are committed to providing better services, saving customers a lot of time and energy.
Service content and process
Service requirements
- Target gene sequence or accession number
- Required vector
- Plant variety and name. If it is a rare plant, the customer needs to prepare it
Feedback to customers
- Sequencing report of constructed vector.
- The plasmid and bacteria of the constructed vector.
- Complete experimental methods, original experimental records, instrument parameters, reagents and consumables information.
- Phenotype and gene function analysis pictures (customized according to requirements).
Project cycle
- It takes about 4 months.
Advantages
- Stable and easy. The target gene fragment can be assembled by cloning directly into a viral vector without assembling the inverted repeat sequence, and the inverted repeat sequence may be unstable when propagating in a bacterial host.
- Simple and fast. VIGS vectors are usually introduced into plants by Agrobacterium-mediated methods or bio bombardment methods, and the VIGS phenotype develops within 1 or 2 weeks.
- The cost is lower. The cost of VIGS experiments is relatively low, and high-throughput screening research can be achieved.
- We have many newly developed virus-derived vectors, such as tobacco mosaic virus (TMV), potato X virus (PVX), tobacco rattle virus (TRV), tomato golden mosaic virus (TGMV), African cassava mosaic virus (ACMV).
- There are also many mature plant transformation platforms, such as tobacco, Arabidopsis, rice, wheat, corn, soybean, cotton, etc.
※ It should be noted that our service is only used for research.
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