Services
- Genetic Marker Assisted Breeding
- Plant Molecular Biology Research
- Research on DNA Level of Plant
- Research on Microspore Embryogenesis
- Plant Genetic Engineering
- Plant Genome Editing with CRISPR / Cas9
- Plant Genome Editing with TALEN
- Plant Genome Editing with ZFN
- RNAi Mediated Plant Gene Silencing
- Overexpression of Plant Genes
- Arabidopsis thaliana Transformation
- Oryza sativa Transformation
- Zea mays Transformation
- Triticum aestivum Transformation
- Medicago truncatula Transformation
- Glycine max Transformation
- Gossypium hirsutum Transformation
- Nicotiana tabacum Transformation
- Solanum lycopersicum Transformation
- Brassica napus Transformation
- Solanum tuberosum Transformation
- Virus-Induced Gene Silencing (VIGS) of Plant Genes
- Research on Protein Level of Plant
- Plant Epigenetic Modification Testing Services
- Sequencing-based Plant Breeding
- Plant DNA-level Sequencing Services
- Plant Genome De Novo Service
- Plant Whole Genome Resequencing Service
- Plant Reduced-Representation Genome Sequencing (RRGS)
- Plant Genetic Map Service
- BSA Trait Positioning of Plant
- Genome-Wide Association Study (GWAS) of Plant
- eQTL Analysis of Plant
- Plant Genetic Evolution Service
- Plant Pan-genome Sequencing
- Plant Whole Exome Sequencing Service
- Individual Selection Pressure Analysis of Plant
- Mixing-tank Selection Pressure Analysis of Plant
- Plant Whole Genome Survey
- Plant RNA Level Sequencing Services
- Eukaryotic Transcriptome Sequencing without Reference Genome
- Eukaryotic Transcriptome Sequencing with Reference Genome
- Prokaryotic Transcriptome Sequencing Analysis
- LncRNA Sequencing of Plant
- Plant Small RNA Sequencing
- Plant Circular RNA Sequencing
- Plant Comparative Transcriptome Service
- Plant Isoform-sequencing with Reference Genome
- Plant Isoform-sequencing without Reference Genome
- Ribo-seq of Plant
- Metatranscriptome Sequencing of Plant
- Plant Single Cell Level Sequencing Services
- Plant Epigenetics Level Sequencing Services
- Plant Proteomics Service
- Proteomics Qualitative Analysis in Plant
- Plant Protein Quantitative Analysis Service Based on Isotope Labeling (iTRAQ / TMT)
- Non-labeled Plant Protein Quantitative Analysis (Label-free / DIA)
- Plant Protein Targeted Quantitative Service (PRM / MRM / AQUA)
- Post-translational Modification Proteomics (PTMs) Service for Plant
- Plant Metabolomics Services
- Plant DNA-level Sequencing Services
- Other Services
- Plant CNV Analysis Service
- Plant Mutation Detection Service
- Plant Strain / Cell Level Services
- Plant Tissue and Cell Culture Services
- Plant Polyploidization Services
- Plant Haploidization Services
- Plant Phenotypic Analysis
- Plant Stress Response Indicators Analysis
- Plant Biochemical Analysis
- Plant Tissue and Cell Imaging Services
- Plant Disease Identification Services
- Plant Organelle Isolation Services
- Genetically Modified Plant Testing Services
- Seed Testing Services
eQTL Analysis of Plant
INQUIRYINTRODUCTIONS
The full name of eQTL is expression quantitative trait Loci. its specific principle is to use the expression data of each genotype from a segregated population as a quantitative trait to study the correlation between genetic mutations and gene expression. The essence of eQTL data analysis is linkage analysis based on family populations or whole-genome association analysis based on natural populations, using gene expression information (RNA expression abundance detected by transcriptome) as the phenotype to carry out the correlation between gene expression and genotype sexual analysis. Through eQTL analysis, we can analyze the regulatory relationship of gene (DNA) mutations on gene expression, and further analyze the regulatory mechanism of complex traits from the perspective of gene mutation→gene expression→character change. The application in agricultural molecular breeding is mainly to study the regulation mechanism of various quantitative traits of crops.
SERVICES
With years of rich experience, Lifeasible provides eQTL analysis service. We provide a one-stop service model. We complete a series of processes from sample extraction, quality testing, sequencing, biological information analysis to making reports.
Workflow of eQTL analysis service
Analysis content
- Standard analysis
Variation (SNP) detection
Expression detection
eQTL analysis
- Advanced analysis
Statistics of eQTL and target genes
cis/trans QTL classification and statistics
Functional enrichment analysis of eQTL locus-related genes
eQTL hot spot analysis
- Personality analysis
Specific types of eQTL analysis (such as splicing eQTL, response eQTL)
Comparison of different eQTL networks
Analysis of the upstream and downstream of key eQTL loci
Comparison of eQTL SNP and phenotype GWAS SNP
Sample requirements
- Whole genome resequencing
DNA concentration≥30ng/ul, total amount≥6μg, OD260/280=1.8~2.0
- Transcriptome sequencing
Subject to the corresponding transcriptome sequencing requirements
- The recommended sample size is greater than 300
Project cycle
- The entire project generally takes about 2.5 months. The specific completion time depends on the specific scale of the project (number of samples and number of phenotype types).
Feedback to customers
- Raw data
- A project report includes experimental process, instrument parameters, analysis methods and results, etc.
Service process
ADVANTAGES
- eQTL analysis uses the advanced illumina sequencing platform to quickly and efficiently read high-quality sequencing data.
- Lifeasible has a high-performance computing platform (HPC) that uses an efficient combination of DELL computing nodes and Isilon storage to achieve fast and stable sequencing data analysis and delivery.
- Our experienced researchers help you with scientific plan design.
- Personalized customized package service.
※ It should be noted that our service is only used for research.
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