Services
- Genetic Marker Assisted Breeding
- Plant Molecular Biology Research
- Research on DNA Level of Plant
- Research on Microspore Embryogenesis
- Plant Genetic Engineering
- Plant Genome Editing with CRISPR / Cas9
- Plant Genome Editing with TALEN
- Plant Genome Editing with ZFN
- RNAi Mediated Plant Gene Silencing
- Overexpression of Plant Genes
- Arabidopsis thaliana Transformation
- Oryza sativa Transformation
- Zea mays Transformation
- Triticum aestivum Transformation
- Medicago truncatula Transformation
- Glycine max Transformation
- Gossypium hirsutum Transformation
- Nicotiana tabacum Transformation
- Solanum lycopersicum Transformation
- Brassica napus Transformation
- Solanum tuberosum Transformation
- Virus-Induced Gene Silencing (VIGS) of Plant Genes
- Research on Protein Level of Plant
- Plant Epigenetic Modification Testing Services
- Sequencing-based Plant Breeding
- Plant DNA-level Sequencing Services
- Plant Genome De Novo Service
- Plant Whole Genome Resequencing Service
- Plant Reduced-Representation Genome Sequencing (RRGS)
- Plant Genetic Map Service
- BSA Trait Positioning of Plant
- Genome-Wide Association Study (GWAS) of Plant
- eQTL Analysis of Plant
- Plant Genetic Evolution Service
- Plant Pan-genome Sequencing
- Plant Whole Exome Sequencing Service
- Individual Selection Pressure Analysis of Plant
- Mixing-tank Selection Pressure Analysis of Plant
- Plant Whole Genome Survey
- Plant RNA Level Sequencing Services
- Eukaryotic Transcriptome Sequencing without Reference Genome
- Eukaryotic Transcriptome Sequencing with Reference Genome
- Prokaryotic Transcriptome Sequencing Analysis
- LncRNA Sequencing of Plant
- Plant Small RNA Sequencing
- Plant Circular RNA Sequencing
- Plant Comparative Transcriptome Service
- Plant Isoform-sequencing with Reference Genome
- Plant Isoform-sequencing without Reference Genome
- Ribo-seq of Plant
- Metatranscriptome Sequencing of Plant
- Plant Single Cell Level Sequencing Services
- Plant Epigenetics Level Sequencing Services
- Plant Proteomics Service
- Proteomics Qualitative Analysis in Plant
- Plant Protein Quantitative Analysis Service Based on Isotope Labeling (iTRAQ / TMT)
- Non-labeled Plant Protein Quantitative Analysis (Label-free / DIA)
- Plant Protein Targeted Quantitative Service (PRM / MRM / AQUA)
- Post-translational Modification Proteomics (PTMs) Service for Plant
- Plant Metabolomics Services
- Plant DNA-level Sequencing Services
- Other Services
- Plant CNV Analysis Service
- Plant Mutation Detection Service
- Plant Strain / Cell Level Services
- Plant Tissue and Cell Culture Services
- Plant Polyploidization Services
- Plant Haploidization Services
- Plant Phenotypic Analysis
- Plant Stress Response Indicators Analysis
- Plant Biochemical Analysis
- Plant Tissue and Cell Imaging Services
- Plant Disease Identification Services
- Plant Organelle Isolation Services
- Genetically Modified Plant Testing Services
- Seed Testing Services
CLIP-seq in Plant
INQUIRYIntroductions
CLIP-seq (crosslinking-immunprecipitation and high-throughput sequencing) is a high-throughput study of the binding mode of RNA Binding protein (RNA Binding protein RBP) to many RNA targets in vivo, and reveals its function in some important biological processes. It is a revolutionary technology that reveals the interaction between RNA molecules and RBP at the whole genome level. It has also been applied to miRNA target identification, IncRNA biological function research and other work. CLIP-seq has a wide range of applications: (1) Draw genome-wide RNA and RBP interaction maps, and analyze the interaction of "Argonaute (Ago)-miRNA-mRNA". (2) Analyze the interaction network of non-coding RNA such as RBP and miRNA / IncRNA. (3) Compared with RIP-seq, it can identify the direct interaction between RBP and RNA, and determine the binding site of RBP with miRNA, IncRNA and other Beibian and PNA. Because of its universal application in the above aspects, it is also very necessary to assist related molecular research in the field of agricultural molecular breeding. It can provide theoretical evidence for academic research projects that explain environmental stress, disease resistance, etc.
Services
With years of rich experience, Lifeasible provides crosslinking-immunprecipitation and high-throughput sequencing (CLIP-seq) and analysis services. Customers only need to provide experimental samples, and we complete the entire service process from sample extraction, quality testing, sequencing, biological information analysis to making reports.
Workflow of CLIP-seq in plant
Analysis content
| Standardized analysis | Preprocessing of sequencing data Data output statistics Data quality control to obtain high-quality clean data |
| Align with reference sequence Reads distribution map in the whole transcriptome to identify RNA sites that bind to proteins | |
|
Identify RNA sites that bind to proteins Peak quantity and length distribution statistics Peak distribution map on functional elements | |
| Combined peak analysis and differential combined peak analysis Peak related gene annotation GO/KEGG analysis of peak related genes Peak's motif analysis | |
| Personalized analysis | Combine with supporting RNA-seq data to analyze the regulatory mechanism of the target protein |
| Other personalized analysis |
Sample requirements
- 2x108 cells
- Dry tissue weight ＞500 mg
Project cycle
- It takes 60~90 working days to complete the standard process.
Feedback to customers
- Raw data
- A project report includes experimental process, instrument parameters, analysis methods and results, etc.
Service process
Advantages
- Lifeasible specializes in providing services related to agricultural molecular breeding, with standardized systems and scientific management.
- The experiment adopts a carefully optimized experimental procedure, which has extremely high efficiency and specificity.
- With a strong bioinformatics team, it can meet the various in-depth data analysis needs of customers.
- The one-stop service we provide makes your upstream and downstream experiments seamlessly connected, facilitates your experiments more conveniently and quickly.
- Personalized customized package service.
※ It should be noted that our service is only used for research.
Online Inquiry
