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Chestnut Yield Breeding

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Chestnut is an important woody food crop widely grown in mountainous areas due to its high nutritional value and tolerance to poor soils. Chestnut research focuses mainly on resistance, but little research is on traits directly related to yield, etc. Lifeasible, as an advanced agrobiology company, provides advanced technology platforms and specialized solutions for chestnut research. We welcome all chestnut research companies and researchers to cooperate with us to promote the rapid development of chestnut research together.

Explore Our Services of Chestnut Yield Breeding

Molecular marker development

Based on the whole genome sequence of chestnut, we develop high-throughput SSR markers and build a molecular marker system covering the whole genome. For example, we screen SSR markers significantly associated with yield traits such as single grain weight, bract weight, and fruiting rate. We also provide marker-trait association analysis services to help customers quickly locate the target gene locus and shorten the breeding cycle.

Genomics and functional gene mining

We have rich experience in the functional analysis of key genes related to chestnut yield.

Utilizing chestnut genomic information, we have mined yield-related functional genes, such as gibberellin synthesis genes (KAO) regulating male inflorescence development, embryo development genes, and energy metabolism genes.

We focus on providing chestnut gene editing services for targeted improvement of target traits, including shortening of male inflorescence and increasing ovule survival to regulate chestnut yield.

Combined transcriptome and metabolome analysis

We combine high-throughput sequencing technology to analyze the gene expression network at key stages of chestnut flower bud differentiation and embryo development to help researchers screen candidate genes for high yield.

Optimization of mutagenesis breeding technology

We can provide physical and chemical mutation breeding services to create high-yielding mutants according to customers' research needs, and screen the mutant progeny with molecular markers, which can help to target beneficial mutations quickly.

In addition, we are constantly optimizing and updating our chestnut molecular breeding technology, including establishing a phenomics and intelligent analysis platform to accelerate the breeding process.

Our Advanced Technology Platform

Genome-wide association analysis (GWAS) technology platform GWAS is a technology that locates genes associated with complex traits by analyzing genome-wide associations of molecular markers (e.g., SNPs) with phenotypic traits. We use GWAS to analyze yield-related traits (e.g., fruit weight) and identify QTLs with significant associations.GWAS is characterized by high resolution, genome-wide coverage, and the ability to handle genetic diversity in natural populations.
In vitro culture and gene editing technology platform We have established a tissue culture technology platform to help researchers promote the rapid propagation of good chestnut seeds. In addition, we further provide a platform for chestnut gene editing breeding by optimizing the healing tissue transient transformation system.
Gene silencing and overexpression technology platform Candidate genes screened by GWAS need to be functionally verified to clarify their mechanism of action, and gene silencing (e.g., RNA interference) and overexpression are the core tools. For example, gene silencing and overexpression technologies are used to verify their functions, and we found that CmAP2 affects the size of chestnut seeds by negatively regulating cell size. In contrast, CmCIB1 affects the length of chestnut leaves and new shoots by affecting the length of the cells.

Why Choose Us?

  • We have an advanced chestnut yield breeding technology platform.
  • We support full process customization service for chestnut molecular breeding.
  • We have a professional, experienced gene editing technology team.
  • We provide reliable, professional, and high-quality technical services.

Lifeasible specializes in chestnut molecular breeding. We can provide technical support and solutions for chestnut yield research in multiple dimensions, ranging from germplasm resource identification to precision breeding. If you are interested in us, please feel free to contact us.

※ For research or industrial use.

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