Plant Southern Blot Hybridization Service

INQUIRY

Introductions

When detect the safety of genetically modified biological products, Southern hybridization will be performed on plants or plant products to confirm the structural stability of their genomic DNA and whether clonal rearrangement occurs. Southern blotting can confirm the identity of the target matched with the hybridization probe, and the size and abundance comparable to the molecular weight of the marker. The principle of southern blot hybridization is very simple. First, the DNA fragments were digested with restriction enzymes, and then separated by agarose gel electrophoresis. Secondly, the DNA on the gel is denatured and the single-stranded DNA fragments are transferred to nylon membrane or other solid support in situ. Then it is fixed by dry baking or UV irradiation, and then hybridized with the DIG-labeled probe of the corresponding structure. Finally, autoradiography or enzyme reaction is used to display the color to detect the content of specific DNA molecules.

Services

As a leading agricultural molecular breeding company,Lifeasible provides a variety of cutting-edge agricultural biotechnology, suchas experimental methods and technologies for identifying and characterizingplant genes or proteins. Many studies have been carried out at nucleic acids level, aiming to understand the biological functions of plant genes, and to master these functions for the regulation of plant growth, resistance to adversity, prevention and treatment of diseases, and the cultivation and selection of required plant traits. As part of its trait introgression services, Lifeasible has established a southern hybridization platform to meet your related needs and serve you wholeheartedly.  

Workflow of southern blot hybridization

Provided by customer

• Bacteria liquid
  No less than 500ul, transported on ice bag or dry ice.
• Organization
  The total amount is not less than 100mg, transported on dry ice.
• Cells
  Just provide fresh cells, transported on dry ice.
• DNA
  There must be no degradation, no protein contamination, sufficient total amount and concentration of DNA, and electrophoresis detection pictures attached.
• Please indicate restriction enzyme. If the project uses unconventional restriction enzymes, additional fees will be charged, or the restriction enzymes may be provided by the customer.

Provided by Lifeasible

• Remaining template and primers
• Experiment report (detailed operation steps, image, result analysis, etc.)

Advantages

• The probe is labeled with DIG (Digoxin), which has the advantages of low noise, high sensitivity, and no radioactive contamination.
• The original data and experimental materials are retained during the experiment, and the data is true and credible.
• The experimental results are stable and repeatable.
• Combining traditional film exposure with a cutting-edge automated chemiluminescence imaging detection system ensures that imaging time points with a better signal-to-noise ratio are captured, and high-quality experimental results can be delivered to customers. Customers can directly use the experimental data provided by us to submit articles.

 

※ It should be noted that our service is only used for research.

Related Sections

• Plant Northern Blot Hybridization Service
• Plant Dot Blot Hybridization Service
• Plant In Situ Hybridization Service
• Plant Gene Chip Service

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