Services
- Genetic Marker Assisted Breeding
- Plant Molecular Biology Research
- Research on DNA Level of Plant
- Research on Microspore Embryogenesis
- Plant Genetic Engineering
- Plant Genome Editing with CRISPR / Cas9
- Plant Genome Editing with TALEN
- Plant Genome Editing with ZFN
- RNAi Mediated Plant Gene Silencing
- Overexpression of Plant Genes
- Arabidopsis thaliana Transformation
- Oryza sativa Transformation
- Zea mays Transformation
- Triticum aestivum Transformation
- Medicago truncatula Transformation
- Glycine max Transformation
- Gossypium hirsutum Transformation
- Nicotiana tabacum Transformation
- Solanum lycopersicum Transformation
- Brassica napus Transformation
- Solanum tuberosum Transformation
- Virus-Induced Gene Silencing (VIGS) of Plant Genes
- Research on Protein Level of Plant
- Plant Epigenetic Modification Testing Services
- Sequencing-based Plant Breeding
- Plant DNA-level Sequencing Services
- Plant Genome De Novo Service
- Plant Whole Genome Resequencing Service
- Plant Reduced-Representation Genome Sequencing (RRGS)
- Plant Genetic Map Service
- BSA Trait Positioning of Plant
- Genome-Wide Association Study (GWAS) of Plant
- eQTL Analysis of Plant
- Plant Genetic Evolution Service
- Plant Pan-genome Sequencing
- Plant Whole Exome Sequencing Service
- Individual Selection Pressure Analysis of Plant
- Mixing-tank Selection Pressure Analysis of Plant
- Plant Whole Genome Survey
- Plant RNA Level Sequencing Services
- Eukaryotic Transcriptome Sequencing without Reference Genome
- Eukaryotic Transcriptome Sequencing with Reference Genome
- Prokaryotic Transcriptome Sequencing Analysis
- LncRNA Sequencing of Plant
- Plant Small RNA Sequencing
- Plant Circular RNA Sequencing
- Plant Comparative Transcriptome Service
- Plant Isoform-sequencing with Reference Genome
- Plant Isoform-sequencing without Reference Genome
- Ribo-seq of Plant
- Metatranscriptome Sequencing of Plant
- Plant Single Cell Level Sequencing Services
- Plant Epigenetics Level Sequencing Services
- Plant Proteomics Service
- Proteomics Qualitative Analysis in Plant
- Plant Protein Quantitative Analysis Service Based on Isotope Labeling (iTRAQ / TMT)
- Non-labeled Plant Protein Quantitative Analysis (Label-free / DIA)
- Plant Protein Targeted Quantitative Service (PRM / MRM / AQUA)
- Post-translational Modification Proteomics (PTMs) Service for Plant
- Plant Metabolomics Services
- Plant DNA-level Sequencing Services
- Other Services
- Plant CNV Analysis Service
- Plant Mutation Detection Service
- Plant Strain / Cell Level Services
- Plant Tissue and Cell Culture Services
- Plant Polyploidization Services
- Plant Haploidization Services
- Plant Phenotypic Analysis
- Plant Stress Response Indicators Analysis
- Plant Biochemical Analysis
- Plant Tissue and Cell Imaging Services
- Plant Disease Identification Services
- Plant Organelle Isolation Services
- Genetically Modified Plant Testing Services
- Seed Testing Services
Plant Genome Editing with ZFN
INQUIRYIntroductions
Zinc finger nucleases (ZFNs) are the earliest artificially synthesized restriction endonucleases used for genome editing, which can realize the insertion, deletion or replacement of gene sequences. Compared with traditional plant breeding technology, ZFNs gene editing technology has higher accuracy. For example, using ZFN-assisted gene targeting can introduce two herbicide resistance genes (tobacco acetolactate synthase SuRA and SuRB) into crops to increase the crop's resistance to herbicides. Zinc finger nuclease contains two parts: DNA binding domain composed of zinc finger protein and FokI with restriction endonuclease activity. When a pair of ZFNs bind to the nucleotide sites of double-stranded DNA, FokI cuts the double-stranded DNA, causing the DNA to produce a double-strand break at that site. The broken DNA is repaired by the cell's own non-homologous end joining (NHEJ) or homologous recombination repair (HDR), resulting in the editing of nucleotide insertions, deletions, substitutions or point mutations.
Schematic diagram showing structure and design of a typical ZFN (Raikwar S P, et al., 2016)
Services
Plant genome editing with ZFN has a wide range of applications in agricultural molecular breeding, such as functional genomics research and genetic trait improvement. Lifeasible provides plant gene editing service with ZFN. With a professional team and extensive experience we will work closely with you to ensure that your project is completed efficiently and provide high-quality data.
Service content
- Target sequence selection
- ZFNs design
- ZFNs vector construction
- Plasmid transformation
- Obtaining genetically modified plants
Customer provided
- Species
- Gene name or gene ID
Delivery standard
- Genetically modified plants
- Images
- Complete project report (materials, reagents, instruments, methods, data analysis, experimental results)
Project cycle
- Takes about 2~3 months
Advantages
- Rapid design, assembly and verification of ZFN pairs for target genes
- Diversification of zinc finger protein design technology
- Mature plant transgenic technology system
- The service quality is high and the cycle is short
- With modern equipment, experienced team, cutting-edge technology
A variety of zinc finger protein construction technologies have been successfully developed, such as oligomerized pool engineering system (OPEN), phage display technology, bacterial one-hybrid, modular assembly, etc.
It can provide transgenic services for many species such as Arabidopsis, rice, wheat, tomato, alfalfa, corn, cotton, sorghum, soybean, sugar beet, cabbage, tobacco, eggplant, cucumber, potato, petunia and rape.
Reference
- Raikwar S P, Raikwar A S, Chaurasia S S, et al. Gene editing for corneal disease management[J]. World Journal of Translational Medicine, 2016, 5(1):1.
※ It should be noted that our service is only used for research.
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