Services
- Genetic Marker Assisted Breeding
- Plant Molecular Biology Research
- Research on DNA Level of Plant
- Research on Microspore Embryogenesis
- Plant Genetic Engineering
- Plant Genome Editing with CRISPR / Cas9
- Plant Genome Editing with TALEN
- Plant Genome Editing with ZFN
- RNAi Mediated Plant Gene Silencing
- Overexpression of Plant Genes
- Arabidopsis thaliana Transformation
- Oryza sativa Transformation
- Zea mays Transformation
- Triticum aestivum Transformation
- Medicago truncatula Transformation
- Glycine max Transformation
- Gossypium hirsutum Transformation
- Nicotiana tabacum Transformation
- Solanum lycopersicum Transformation
- Brassica napus Transformation
- Solanum tuberosum Transformation
- Virus-Induced Gene Silencing (VIGS) of Plant Genes
- Research on Protein Level of Plant
- Plant Epigenetic Modification Testing Services
- Sequencing-based Plant Breeding
- Plant DNA-level Sequencing Services
- Plant Genome De Novo Service
- Plant Whole Genome Resequencing Service
- Plant Reduced-Representation Genome Sequencing (RRGS)
- Plant Genetic Map Service
- BSA Trait Positioning of Plant
- Genome-Wide Association Study (GWAS) of Plant
- eQTL Analysis of Plant
- Plant Genetic Evolution Service
- Plant Pan-genome Sequencing
- Plant Whole Exome Sequencing Service
- Individual Selection Pressure Analysis of Plant
- Mixing-tank Selection Pressure Analysis of Plant
- Plant Whole Genome Survey
- Plant RNA Level Sequencing Services
- Eukaryotic Transcriptome Sequencing without Reference Genome
- Eukaryotic Transcriptome Sequencing with Reference Genome
- Prokaryotic Transcriptome Sequencing Analysis
- LncRNA Sequencing of Plant
- Plant Small RNA Sequencing
- Plant Circular RNA Sequencing
- Plant Comparative Transcriptome Service
- Plant Isoform-sequencing with Reference Genome
- Plant Isoform-sequencing without Reference Genome
- Ribo-seq of Plant
- Metatranscriptome Sequencing of Plant
- Plant Single Cell Level Sequencing Services
- Plant Epigenetics Level Sequencing Services
- Plant Proteomics Service
- Proteomics Qualitative Analysis in Plant
- Plant Protein Quantitative Analysis Service Based on Isotope Labeling (iTRAQ / TMT)
- Non-labeled Plant Protein Quantitative Analysis (Label-free / DIA)
- Plant Protein Targeted Quantitative Service (PRM / MRM / AQUA)
- Post-translational Modification Proteomics (PTMs) Service for Plant
- Plant Metabolomics Services
- Plant DNA-level Sequencing Services
- Other Services
- Plant CNV Analysis Service
- Plant Mutation Detection Service
- Plant Strain / Cell Level Services
- Plant Tissue and Cell Culture Services
- Plant Polyploidization Services
- Plant Haploidization Services
- Plant Phenotypic Analysis
- Plant Stress Response Indicators Analysis
- Plant Biochemical Analysis
- Plant Tissue and Cell Imaging Services
- Plant Disease Identification Services
- Plant Organelle Isolation Services
- Genetically Modified Plant Testing Services
- Seed Testing Services
Cloning of Plant Genes
INQUIRYIntroductions
Plant genes cloning, also known as plant molecular cloning or recombinant DNA technology, is the application of enzymatic methods. Firstly, DNA molecules from different resources are reassembled into hybrid molecules through (amplification,) restriction digestion, ligation and other operations in vitro. Then make it amplified in an appropriate host cell, and finally form a large number of progeny DNA molecules. Plant DNA cloning involves a series of molecular biology technologies, such as the acquisition of target DNA fragments of plant, the selection of vectors, the selection of various tool enzymes, recombination technology in vitro, host cell introduction technology and recombinant screening technology, etc. Because of the different choices at each step, there are different series of operations. Plant genes cloning is widely used in agricultural molecular breeding, such as develop molecular markers, study gene function, study the relationship between genes and phenotypes and others.
Services
Lifeasible has established a complete plant genes cloning platform to help you carry out relevant experimental services.
Workflow of plant genes cloning
- Obtain the target DNA fragment from crop
- Choose a vector
- Recombination of target fragment and vector in vitro
- Import recipient cells
- Recombinant screening
Provided by customer
- Name and ID of plant gene
- Plant tissue (greater than 100mg / sample, indicate the species information
- PCR products (preferably above 5μg, indicate the concentration, with electrophoresis photos)
- Plasmid (greater than 4μg / sample)
- Vector (indicate the restriction site used for cloning)
- Glycerol bacteria or puncture bacteria (indicate antibiotics and ensure high activity)
Provided by Lifeasible
- Raw data and pictures of the experiment
- Lyophilized powder of plasmid and puncture bacteria
- Experiment report (experimental equipment, reagents, methods, results, conclusions)
Advantages
- We can match different DNA extraction methods according to sample characteristics.
- We will provide many different vectors for you to choose from, and the vectors provided by us have clear information and can be used for other experiments.
- After the project is completed, the results of two forms of plant gene cloning such as puncture bacteria and plasmid freeze-dried powder will be handed to you.
※ It should be noted that our service is only used for research.
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