Services
- Genetic Marker Assisted Breeding
- Plant Molecular Biology Research
- Research on DNA Level of Plant
- Research on Microspore Embryogenesis
- Plant Genetic Engineering
- Plant Genome Editing with CRISPR / Cas9
- Plant Genome Editing with TALEN
- Plant Genome Editing with ZFN
- RNAi Mediated Plant Gene Silencing
- Overexpression of Plant Genes
- Arabidopsis thaliana Transformation
- Oryza sativa Transformation
- Zea mays Transformation
- Triticum aestivum Transformation
- Medicago truncatula Transformation
- Glycine max Transformation
- Gossypium hirsutum Transformation
- Nicotiana tabacum Transformation
- Solanum lycopersicum Transformation
- Brassica napus Transformation
- Solanum tuberosum Transformation
- Virus-Induced Gene Silencing (VIGS) of Plant Genes
- Research on Protein Level of Plant
- Plant Epigenetic Modification Testing Services
- Sequencing-based Plant Breeding
- Plant DNA-level Sequencing Services
- Plant Genome De Novo Service
- Plant Whole Genome Resequencing Service
- Plant Reduced-Representation Genome Sequencing (RRGS)
- Plant Genetic Map Service
- BSA Trait Positioning of Plant
- Genome-Wide Association Study (GWAS) of Plant
- eQTL Analysis of Plant
- Plant Genetic Evolution Service
- Plant Pan-genome Sequencing
- Plant Whole Exome Sequencing Service
- Individual Selection Pressure Analysis of Plant
- Mixing-tank Selection Pressure Analysis of Plant
- Plant Whole Genome Survey
- Plant RNA Level Sequencing Services
- Eukaryotic Transcriptome Sequencing without Reference Genome
- Eukaryotic Transcriptome Sequencing with Reference Genome
- Prokaryotic Transcriptome Sequencing Analysis
- LncRNA Sequencing of Plant
- Plant Small RNA Sequencing
- Plant Circular RNA Sequencing
- Plant Comparative Transcriptome Service
- Plant Isoform-sequencing with Reference Genome
- Plant Isoform-sequencing without Reference Genome
- Ribo-seq of Plant
- Metatranscriptome Sequencing of Plant
- Plant Single Cell Level Sequencing Services
- Plant Epigenetics Level Sequencing Services
- Plant Proteomics Service
- Proteomics Qualitative Analysis in Plant
- Plant Protein Quantitative Analysis Service Based on Isotope Labeling (iTRAQ / TMT)
- Non-labeled Plant Protein Quantitative Analysis (Label-free / DIA)
- Plant Protein Targeted Quantitative Service (PRM / MRM / AQUA)
- Post-translational Modification Proteomics (PTMs) Service for Plant
- Plant Metabolomics Services
- Plant DNA-level Sequencing Services
- Other Services
- Plant CNV Analysis Service
- Plant Mutation Detection Service
- Plant Strain / Cell Level Services
- Plant Tissue and Cell Culture Services
- Plant Polyploidization Services
- Plant Haploidization Services
- Plant Phenotypic Analysis
- Plant Stress Response Indicators Analysis
- Plant Biochemical Analysis
- Plant Tissue and Cell Imaging Services
- Plant Disease Identification Services
- Plant Organelle Isolation Services
- Genetically Modified Plant Testing Services
- Seed Testing Services
Plant Genome De Novo Service
INQUIRYINTRODUCTIONS
Genome de novo sequencing is de novo sequencing, which does not rely on any reference sequence to sequence a species, and then use bioinformatics analysis methods to splice and assemble to obtain a complete gene sequence map of the species. In order to achieve the purpose of promoting crop genetic improvement, de novo provides agricultural scientists and breeders with high-quality, convenient and stable research solutions, covering genome assembly, annotation, evolutionary analysis and other customized analysis and other subdivision directions. It is a cost-controllable molecular breeding tool that can improve breeding efficiency and boost the improvement of the agricultural industry. Plant genome de novo applications have been widely used in various fields, such as genome survey evaluation, whole genome assembly and annotation, and species evolution analysis.
SERVICES
With years of rich experience, Lifeasible provides plant genome de novo service. We provide a one-stop service model. We complete a series of processes from sample extraction, quality testing, sequencing, biological information analysis to making reports.
Technical route
Analysis content
- Data processing
Remove the linker sequence, contamination sequence, etc.
- Genome assembly
Raw data statistics, sequencing depth analysis, coverage statistics, GC content analysis, etc.
- Genome annotation
Gene prediction, gene function annotation, repetitive sequence analysis, non-coding RNA annotation, etc.
- Comparative genomics and evolutionary analysis
Species phylogenetic tree construction, gene family identification, gene collinearity analysis, etc.
- Personalized analysis
Sample requirements
- Sample concentration≥200ng/μl, total amount≥500μg, OD260/280＝1.8~2.0. (The amount of DNA required is related to the number of libraries constructed and the type of library)
Project cycle
- The entire project generally takes about 7 months, and the specific completion time depends on the size and complexity of the genome. The time is calculated from the time the sample is qualified, and does not include the time when the experiment is stalled due to sample problems.
Feedback to customers
- Raw data
- A project report includes experimental process, instrument parameters, analysis methods and results, etc.
Service process
ADVANTAGES
- Our experienced researchers help you with scientific plan design.
- Our method is excellent, the cycle is short, and the accuracy is high.
- Excellent analysis creates project results.
- We combine rich project experience, professional project planning guidance and analysis process to ensure rapid and accurate assessment of genome characteristics.
- Personalized customized package service.
※ It should be noted that our service is only used for research.
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