Services
- Genetic Marker Assisted Breeding
- Plant Molecular Biology Research
- Research on DNA Level of Plant
- Research on Microspore Embryogenesis
- Plant Genetic Engineering
- Plant Genome Editing with CRISPR / Cas9
- Plant Genome Editing with TALEN
- Plant Genome Editing with ZFN
- RNAi Mediated Plant Gene Silencing
- Overexpression of Plant Genes
- Arabidopsis thaliana Transformation
- Oryza sativa Transformation
- Zea mays Transformation
- Triticum aestivum Transformation
- Medicago truncatula Transformation
- Glycine max Transformation
- Gossypium hirsutum Transformation
- Nicotiana tabacum Transformation
- Solanum lycopersicum Transformation
- Brassica napus Transformation
- Solanum tuberosum Transformation
- Virus-Induced Gene Silencing (VIGS) of Plant Genes
- Research on Protein Level of Plant
- Plant Epigenetic Modification Testing Services
- Sequencing-based Plant Breeding
- Plant DNA-level Sequencing Services
- Plant Genome De Novo Service
- Plant Whole Genome Resequencing Service
- Plant Reduced-Representation Genome Sequencing (RRGS)
- Plant Genetic Map Service
- BSA Trait Positioning of Plant
- Genome-Wide Association Study (GWAS) of Plant
- eQTL Analysis of Plant
- Plant Genetic Evolution Service
- Plant Pan-genome Sequencing
- Plant Whole Exome Sequencing Service
- Individual Selection Pressure Analysis of Plant
- Mixing-tank Selection Pressure Analysis of Plant
- Plant Whole Genome Survey
- Plant RNA Level Sequencing Services
- Eukaryotic Transcriptome Sequencing without Reference Genome
- Eukaryotic Transcriptome Sequencing with Reference Genome
- Prokaryotic Transcriptome Sequencing Analysis
- LncRNA Sequencing of Plant
- Plant Small RNA Sequencing
- Plant Circular RNA Sequencing
- Plant Comparative Transcriptome Service
- Plant Isoform-sequencing with Reference Genome
- Plant Isoform-sequencing without Reference Genome
- Ribo-seq of Plant
- Metatranscriptome Sequencing of Plant
- Plant Single Cell Level Sequencing Services
- Plant Epigenetics Level Sequencing Services
- Plant Proteomics Service
- Proteomics Qualitative Analysis in Plant
- Plant Protein Quantitative Analysis Service Based on Isotope Labeling (iTRAQ / TMT)
- Non-labeled Plant Protein Quantitative Analysis (Label-free / DIA)
- Plant Protein Targeted Quantitative Service (PRM / MRM / AQUA)
- Post-translational Modification Proteomics (PTMs) Service for Plant
- Plant Metabolomics Services
- Plant DNA-level Sequencing Services
- Other Services
- Plant CNV Analysis Service
- Plant Mutation Detection Service
- Plant Strain / Cell Level Services
- Plant Tissue and Cell Culture Services
- Plant Polyploidization Services
- Plant Haploidization Services
- Plant Phenotypic Analysis
- Plant Stress Response Indicators Analysis
- Plant Biochemical Analysis
- Plant Tissue and Cell Imaging Services
- Plant Disease Identification Services
- Plant Organelle Isolation Services
- Genetically Modified Plant Testing Services
- Seed Testing Services
Plant Protein Interaction Services
INQUIRYIntroductions
Proteins in organisms interact with each other to participate in all aspects of life processes such as biological signal transmission, gene expression and regulation, energy and material metabolism, and cell cycle regulation. Analyze the interaction relationship between proteins in biological systems have a significance meaning for understand the working principle of proteins in biological systems, understand the reaction mechanism of biological signals and energy substance metabolism under special physiological conditions such as disease and stress resistance, and understand the functional connections between proteins. Therefore, exploring the research methods of protein-protein interactions in plants is of great significance and is also a hot spot in the field of protein research.
Services
Protein interaction studies is to verify whether two proteins interact and to screen for interacting proteins of a protein of interest. Lifeasible provides the following technologies to help researchers analyze the protein interaction network in organisms.
Protein interaction related services
- Yeast two-hybrid, Y2H
Genome-wide screening of protein interactions by co-expression of bait with protein libraries. this assay has become one of the most popular approaches for probing physical interactions in vivo.
- BiMolecular fluorescence complementation, BiFC
It allows direct visualization of protein interactions, as well as their subcellular localizations in living cells. BiFC has been applied in the studies of proteins from a variety of cell types and organisms from different species.
- Pull-down
Confirm the existence of a protein-protein interaction predicted by other research techniques and identify previously unknown protein-protein interactions as an initial screening assay.
- Co-immunoprecipitation, Co-IP
In vivo technique that detects naturally formed protein complex.
- Bio-layer interferometry, BLI
Quantitative and qualitative characterization of biomolecule interactions.
- Tandem affinity purification, TAP
The basic principle is to fuse the TAP tag to the target protein, and then after two-step affinity purification, the fusion protein and its structurally related proteins are obtained. Further analysis and identification by gel electrophoresis and mass spectrometry.
- Fluorescence resonance energy transfer, FRET
It is a physical phenomenon which relies on the distance-dependent energy transfer. Test the direct interaction in vitro of two candidate proteins, suitable for both wild type and mutant proteins.
- Surface plasmon resonance, SPR
The versatile label-free BIACORE technique allows detecting and monitoring of interactions in real time.
- Phage display technology
Obtain optimal protein binding by immobilization of an antigen on magnetic beads, and screening against a phage display library.
- Protein array
This technique is a high-throughput method used to track the interactions and activities of proteins, and determining function on a large scale.
Service process
- Service inquiry and technical consultation
- Design experiment
- Sign contracts and confidentiality agreements with customers
- Update project status
- Organize experimental data and pictures, analyze the results and make a report
Sample requirements
- Different methods have different requirements, please contact us for specific information.
Delivery standard
Project cycle
- The time required for each method is different, it may take 1~2 months.
Advantages
- Discuss with you the details of the expected interaction study, and develop experimental strategies/methods according to your requirements.
- Standardization system, scientific management.
- A variety of protein interaction detection/screening related services for your flexible choice
- Flexible quotation to meet your needs.
- Highly sensitive homogeneous determination.
- Leading technology platform conditions, first-class R&D and production equipment.
※ It should be noted that our service is only used for research.
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