Services
- Genetic Marker Assisted Breeding
- Plant Molecular Biology Research
- Research on DNA Level of Plant
- Research on Microspore Embryogenesis
- Plant Genetic Engineering
- Plant Genome Editing with CRISPR / Cas9
- Plant Genome Editing with TALEN
- Plant Genome Editing with ZFN
- RNAi Mediated Plant Gene Silencing
- Overexpression of Plant Genes
- Arabidopsis thaliana Transformation
- Oryza sativa Transformation
- Zea mays Transformation
- Triticum aestivum Transformation
- Medicago truncatula Transformation
- Glycine max Transformation
- Gossypium hirsutum Transformation
- Nicotiana tabacum Transformation
- Solanum lycopersicum Transformation
- Brassica napus Transformation
- Solanum tuberosum Transformation
- Virus-Induced Gene Silencing (VIGS) of Plant Genes
- Research on Protein Level of Plant
- Plant Epigenetic Modification Testing Services
- Sequencing-based Plant Breeding
- Plant DNA-level Sequencing Services
- Plant Genome De Novo Service
- Plant Whole Genome Resequencing Service
- Plant Reduced-Representation Genome Sequencing (RRGS)
- Plant Genetic Map Service
- BSA Trait Positioning of Plant
- Genome-Wide Association Study (GWAS) of Plant
- eQTL Analysis of Plant
- Plant Genetic Evolution Service
- Plant Pan-genome Sequencing
- Plant Whole Exome Sequencing Service
- Individual Selection Pressure Analysis of Plant
- Mixing-tank Selection Pressure Analysis of Plant
- Plant Whole Genome Survey
- Plant RNA Level Sequencing Services
- Eukaryotic Transcriptome Sequencing without Reference Genome
- Eukaryotic Transcriptome Sequencing with Reference Genome
- Prokaryotic Transcriptome Sequencing Analysis
- LncRNA Sequencing of Plant
- Plant Small RNA Sequencing
- Plant Circular RNA Sequencing
- Plant Comparative Transcriptome Service
- Plant Isoform-sequencing with Reference Genome
- Plant Isoform-sequencing without Reference Genome
- Ribo-seq of Plant
- Metatranscriptome Sequencing of Plant
- Plant Single Cell Level Sequencing Services
- Plant Epigenetics Level Sequencing Services
- Plant Proteomics Service
- Proteomics Qualitative Analysis in Plant
- Plant Protein Quantitative Analysis Service Based on Isotope Labeling (iTRAQ / TMT)
- Non-labeled Plant Protein Quantitative Analysis (Label-free / DIA)
- Plant Protein Targeted Quantitative Service (PRM / MRM / AQUA)
- Post-translational Modification Proteomics (PTMs) Service for Plant
- Plant Metabolomics Services
- Plant DNA-level Sequencing Services
- Other Services
- Plant CNV Analysis Service
- Plant Mutation Detection Service
- Plant Strain / Cell Level Services
- Plant Tissue and Cell Culture Services
- Plant Polyploidization Services
- Plant Haploidization Services
- Plant Phenotypic Analysis
- Plant Stress Response Indicators Analysis
- Plant Biochemical Analysis
- Plant Tissue and Cell Imaging Services
- Plant Disease Identification Services
- Plant Organelle Isolation Services
- Genetically Modified Plant Testing Services
- Seed Testing Services
Mixing-tank Selection Pressure Analysis of Plant
INQUIRYINTRODUCTIONS
Mixed pool selection pressure analysis is based on the population genetic analysis of mixed samples. Its essence is to use resequencing to obtain the SNP variation information of the mixed samples of each subspecies (or strains) of a species, and use SNP information to analyze each subspecies (strains). The genetic evolutionary relationship between each group can be searched for the regions affected by selection pressure in each group, and the potential selected genes can be located, so as to explain the adaptability of the group at the molecular level. In the case of a large number of samples, the mixed sample strategy will be more cost-effective. This technology is mainly involved in the research of natural selection and (or) artificial selection mechanisms.
SERVICES
With years of rich experience, Lifeasible provides mixing-tank selection pressure analysis service. We provide a one-stop service model. We complete a series of processes from sample extraction, quality testing, sequencing, biological information analysis to making reports.
Technical route
Analysis content
- SNP marker screening and filtering
- Group structure analysis
- LD attenuation distance analysis
- Choose clear analysis
- Selected regions analysis (reference genome must be present)
Sample requirements
- Whole genome resequencing
Sample concentration≥30ng/ul, total amount≥6μg, OD260/280=1.8~2.0
- Simplified genome sequencing
Sample concentration≥25ng/μl, total amount≥2μg, OD260/280=1.8~2.0
- Recommended number of samples
It is recommended that each group have more than 10 samples. If the number of samples reaches 30, the calculation of various indicators of group diversity will be more accurate.
Project cycle
- The entire project generally takes about 2~3 months, and the specific completion time depends on samples number and phenotype types.
Feedback to customers
- Raw data
- A project report includes experimental process, instrument parameters, analysis methods and results, etc.
Service process
ADVANTAGES
- From material selection, library construction and sequencing, to data analysis, every step has a scientific and meticulous design to ensure high-quality research results.
- Lifeasible has a high-performance computing platform (HPC) that uses an efficient combination of DELL computing nodes and isilon storage to achieve fast and stable sequencing data analysis and delivery.
- The continuously updated and expanded high-performance computing platform ensures efficient data processing and secure data storage.
- Personalized customized package service.
※ It should be noted that our service is only used for research.
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