Services
- Genetic Marker Assisted Breeding
- Plant Molecular Biology Research
- Research on DNA Level of Plant
- Research on Microspore Embryogenesis
- Plant Genetic Engineering
- Plant Genome Editing with CRISPR / Cas9
- Plant Genome Editing with TALEN
- Plant Genome Editing with ZFN
- RNAi Mediated Plant Gene Silencing
- Overexpression of Plant Genes
- Arabidopsis thaliana Transformation
- Oryza sativa Transformation
- Zea mays Transformation
- Triticum aestivum Transformation
- Medicago truncatula Transformation
- Glycine max Transformation
- Gossypium hirsutum Transformation
- Nicotiana tabacum Transformation
- Solanum lycopersicum Transformation
- Brassica napus Transformation
- Solanum tuberosum Transformation
- Virus-Induced Gene Silencing (VIGS) of Plant Genes
- Research on Protein Level of Plant
- Plant Epigenetic Modification Testing Services
- Sequencing-based Plant Breeding
- Plant DNA-level Sequencing Services
- Plant Genome De Novo Service
- Plant Whole Genome Resequencing Service
- Plant Reduced-Representation Genome Sequencing (RRGS)
- Plant Genetic Map Service
- BSA Trait Positioning of Plant
- Genome-Wide Association Study (GWAS) of Plant
- eQTL Analysis of Plant
- Plant Genetic Evolution Service
- Plant Pan-genome Sequencing
- Plant Whole Exome Sequencing Service
- Individual Selection Pressure Analysis of Plant
- Mixing-tank Selection Pressure Analysis of Plant
- Plant Whole Genome Survey
- Plant RNA Level Sequencing Services
- Eukaryotic Transcriptome Sequencing without Reference Genome
- Eukaryotic Transcriptome Sequencing with Reference Genome
- Prokaryotic Transcriptome Sequencing Analysis
- LncRNA Sequencing of Plant
- Plant Small RNA Sequencing
- Plant Circular RNA Sequencing
- Plant Comparative Transcriptome Service
- Plant Isoform-sequencing with Reference Genome
- Plant Isoform-sequencing without Reference Genome
- Ribo-seq of Plant
- Metatranscriptome Sequencing of Plant
- Plant Single Cell Level Sequencing Services
- Plant Epigenetics Level Sequencing Services
- Plant Proteomics Service
- Proteomics Qualitative Analysis in Plant
- Plant Protein Quantitative Analysis Service Based on Isotope Labeling (iTRAQ / TMT)
- Non-labeled Plant Protein Quantitative Analysis (Label-free / DIA)
- Plant Protein Targeted Quantitative Service (PRM / MRM / AQUA)
- Post-translational Modification Proteomics (PTMs) Service for Plant
- Plant Metabolomics Services
- Plant DNA-level Sequencing Services
- Other Services
- Plant CNV Analysis Service
- Plant Mutation Detection Service
- Plant Strain / Cell Level Services
- Plant Tissue and Cell Culture Services
- Plant Polyploidization Services
- Plant Haploidization Services
- Plant Phenotypic Analysis
- Plant Stress Response Indicators Analysis
- Plant Biochemical Analysis
- Plant Tissue and Cell Imaging Services
- Plant Disease Identification Services
- Plant Organelle Isolation Services
- Genetically Modified Plant Testing Services
- Seed Testing Services
DAP-seq in Plant
INQUIRYIntroductions
DAP-seq (DNA Affinity Purification sequencing) constructs and expresses transcription factor (TF) protein in vitro, binds to target genome fragments, and enriches the genomic DNA fragments bound to the target protein. Through the combination with high-throughput sequencing technology, the DNA products after DAP are sequenced and analyzed, and the DNA binding sites of the target protein are found from the whole genome, and high-throughput data results can be obtained by high-efficiency sequencing methods. DAP can effectively solve the limitation of CHIP technology's lack of specific antibodies for the target protein, and has a wider range of applications. The application of DAP-seq technology in the field of plant molecular breeding mainly focuses on studying the binding sites and functions of transcription factors, judging the type of histone modification at a specific position in the DNA chain, studying the relationship between histone covalent modification and gene expression, etc. It provides theoretical evidence for academic research projects that explain environmental stress, disease resistance, etc.
Services
With years of rich experience, Lifeasible provides DNA Affinity Purification sequencing (DAP-seq) and analysis services. Customers only need to provide experimental samples, and we complete the entire service process from sample extraction, quality testing, sequencing, biological information analysis to making reports.
Workflow of DAP-seq in plant
Analysis content
| Sequencing evaluation - Filter out low-quality data to ensure data quality |
| Peak analysis - Peak calling, Peak length distribution, Peak depth distribution, Peak enrichment multiple distribution |
| Peak annotation - Peak-related gene analysis, Peak distribution on gene functional elements, GO/Pathway function enrichment analysis of Peak-related genes |
| Motif analysis |
| Peak merging and multi-sample clustering in each treatment group - peak abundance calculation, PCA analysis, correlation heat map analysis |
| Analysis of shared/unique peaks of the treatment group - statistics of shared/unique peaks of the comparison group and related genes, GO/KO enrichment analysis |
| Peak difference analysis between groups - peak related gene analysis between group differences, GO/KO enrichment analysis |
Sample requirements
- Target protein carrier plasmid ≥ 12µg (concentration ≥ 200ng/µl)
- DNA ≥ 1µg (concentration ≥ 30ng/µl)
- Tissue sample ≥ 1g
Project cycle
- It takes 60~90 working days to complete the standard process.
Feedback to customers
- Raw data
- A project report includes experimental process, instrument parameters, analysis methods and results, etc.
Service process
Advantages
- Lifeasible specializes in providing services related to agricultural molecular breeding, with standardized systems and scientific management.
- The experiment adopts a carefully optimized experimental procedure, which has extremely high efficiency and specificity.
- With a strong bioinformatics team, it can meet the various in-depth data analysis needs of customers.
- The one-stop service we provide makes your upstream and downstream experiments seamlessly connected, facilitates your experiments more conveniently and quickly.
- Personalized customized package service.
※ It should be noted that our service is only used for research.
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