Services
- Genetic Marker Assisted Breeding
- Plant Molecular Biology Research
- Research on DNA Level of Plant
- Research on Microspore Embryogenesis
- Plant Genetic Engineering
- Plant Genome Editing with CRISPR / Cas9
- Plant Genome Editing with TALEN
- Plant Genome Editing with ZFN
- RNAi Mediated Plant Gene Silencing
- Overexpression of Plant Genes
- Arabidopsis thaliana Transformation
- Oryza sativa Transformation
- Zea mays Transformation
- Triticum aestivum Transformation
- Medicago truncatula Transformation
- Glycine max Transformation
- Gossypium hirsutum Transformation
- Nicotiana tabacum Transformation
- Solanum lycopersicum Transformation
- Brassica napus Transformation
- Solanum tuberosum Transformation
- Virus-Induced Gene Silencing (VIGS) of Plant Genes
- Research on Protein Level of Plant
- Plant Epigenetic Modification Testing Services
- Sequencing-based Plant Breeding
- Plant DNA-level Sequencing Services
- Plant Genome De Novo Service
- Plant Whole Genome Resequencing Service
- Plant Reduced-Representation Genome Sequencing (RRGS)
- Plant Genetic Map Service
- BSA Trait Positioning of Plant
- Genome-Wide Association Study (GWAS) of Plant
- eQTL Analysis of Plant
- Plant Genetic Evolution Service
- Plant Pan-genome Sequencing
- Plant Whole Exome Sequencing Service
- Individual Selection Pressure Analysis of Plant
- Mixing-tank Selection Pressure Analysis of Plant
- Plant Whole Genome Survey
- Plant RNA Level Sequencing Services
- Eukaryotic Transcriptome Sequencing without Reference Genome
- Eukaryotic Transcriptome Sequencing with Reference Genome
- Prokaryotic Transcriptome Sequencing Analysis
- LncRNA Sequencing of Plant
- Plant Small RNA Sequencing
- Plant Circular RNA Sequencing
- Plant Comparative Transcriptome Service
- Plant Isoform-sequencing with Reference Genome
- Plant Isoform-sequencing without Reference Genome
- Ribo-seq of Plant
- Metatranscriptome Sequencing of Plant
- Plant Single Cell Level Sequencing Services
- Plant Epigenetics Level Sequencing Services
- Plant Proteomics Service
- Proteomics Qualitative Analysis in Plant
- Plant Protein Quantitative Analysis Service Based on Isotope Labeling (iTRAQ / TMT)
- Non-labeled Plant Protein Quantitative Analysis (Label-free / DIA)
- Plant Protein Targeted Quantitative Service (PRM / MRM / AQUA)
- Post-translational Modification Proteomics (PTMs) Service for Plant
- Plant Metabolomics Services
- Plant DNA-level Sequencing Services
- Other Services
- Plant CNV Analysis Service
- Plant Mutation Detection Service
- Plant Strain / Cell Level Services
- Plant Tissue and Cell Culture Services
- Plant Polyploidization Services
- Plant Haploidization Services
- Plant Phenotypic Analysis
- Plant Stress Response Indicators Analysis
- Plant Biochemical Analysis
- Plant Tissue and Cell Imaging Services
- Plant Disease Identification Services
- Plant Organelle Isolation Services
- Genetically Modified Plant Testing Services
- Seed Testing Services
Non-targeted Metabolomics Service in Plant
INQUIRYIntroductions
Non-targeted metabolomics is a technique for systematic qualitative and quantitative analysis of the metabolome of the entire living body on the basis of limited background knowledge. And compare the differences of metabolites between different samples, and screen out significant differences in metabolites with important biological significance between different groups. Since the metabolome is most affected by environmental changes, and the impact of the environment on organisms is ultimately reflected in changes in metabolites, metabonomics research is becoming more and more widely used in the fields of plant growth and development, stress response, color improvement and Breeding, etc.
Services
Under what circumstances are our non-targeted metabolomics services preferred?
- Metabolites of no clear concern
- Want to know what are the metabolites of the control group and the experimental group
- Want to find the difference metabolites between the control group and the experimental group
- Want to find key metabolic pathways and study Molecular mechanisms
Workflow of non-targeted metabolomics in plant
Analysis content
| Standard information analysis | Raw data preprocessing (filtering, de-redundancy, peak identification, peak area integration, integration correction) |
| Metabolite identification | |
| Metabolite quantification | |
| PCA analysis | |
| (O)PLS-DA analysis | |
| Differential metabolite screening | |
| KEGG pathway enrichment analysis of differential metabolites | |
| Customized information analysis | Metabolite regulatory network analysis |
| Multi-omics association analysis (relevant data of other omics: transcriptome, proteome, genome, etc.) |
Sample requirements
- Roots, stems, leaves, fruits, seeds in plants> 200mg fresh weight/sample.
- Store at -80°C after quick freezing in liquid nitrogen. Shipped with dry ice.
- It is recommended that each experimental treatment has more than 6 biological replicates
For other sample types, please consult technical support or sales
Project cycle
- It takes 60~90 working days to complete the standard process.
Feedback to customers
- Raw data
- A project report includes experimental procedures, related mass spectrometry parameters, partial mass spectrometry pictures, raw data, and metabolomics analysis results
Service process
Advantages
- Lifeasible specializes in providing services related to agricultural molecular breeding, with standardized systems and scientific management.
- The experiment adopts a carefully optimized experimental procedure, which has extremely high efficiency and specificity.
- The one-stop service we provide makes your upstream and downstream experiments seamlessly connected, facilitates your experiments more conveniently and quickly.
- With a strong bioinformatics team, it can meet the various in-depth data analysis needs of customers.
- Personalized customized package service.
※ It should be noted that our service is only used for research.
Online Inquiry
