Services

Non-targeted Metabolomics Service in Plant

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Introductions

Non targeted Metabolomics Service

Non-targeted metabolomics is a technique for systematic qualitative and quantitative analysis of the metabolome of the entire living body on the basis of limited background knowledge. And compare the differences of metabolites between different samples, and screen out significant differences in metabolites with important biological significance between different groups. Since the metabolome is most affected by environmental changes, and the impact of the environment on organisms is ultimately reflected in changes in metabolites, metabonomics research is becoming more and more widely used in the fields of plant growth and development, stress response, color improvement and Breeding, etc.

Services

Under what circumstances are our non-targeted metabolomics services preferred?

  • Metabolites of no clear concern
  • Want to know what are the metabolites of the control group and the experimental group
  • Want to find the difference metabolites between the control group and the experimental group
  • Want to find key metabolic pathways and study Molecular mechanisms

Workflow of non-targeted metabolomics in plant

Workflow of non-targeted metabolomics in plant

Analysis content

Standard information analysis Raw data preprocessing (filtering, de-redundancy, peak identification, peak area integration, integration correction)
Metabolite identification
Metabolite quantification
PCA analysis
(O)PLS-DA analysis
Differential metabolite screening
KEGG pathway enrichment analysis of differential metabolites
Customized information analysis Metabolite regulatory network analysis
Multi-omics association analysis (relevant data of other omics: transcriptome, proteome, genome, etc.)

Sample requirements

  • Roots, stems, leaves, fruits, seeds in plants> 200mg fresh weight/sample.
  • For other sample types, please consult technical support or sales

  • Store at -80°C after quick freezing in liquid nitrogen. Shipped with dry ice.
  • It is recommended that each experimental treatment has more than 6 biological replicates

Project cycle

  • It takes 60~90 working days to complete the standard process.

Feedback to customers

  • Raw data
  • A project report includes experimental procedures, related mass spectrometry parameters, partial mass spectrometry pictures, raw data, and metabolomics analysis results

Service process

Service process of non-targeted metabolomics in plant

Advantages

  • Lifeasible specializes in providing services related to agricultural molecular breeding, with standardized systems and scientific management.
  • The experiment adopts a carefully optimized experimental procedure, which has extremely high efficiency and specificity.
  • The one-stop service we provide makes your upstream and downstream experiments seamlessly connected, facilitates your experiments more conveniently and quickly.
  • With a strong bioinformatics team, it can meet the various in-depth data analysis needs of customers.
  • Personalized customized package service.

※ For research or industrial use.

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