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- BBM-BAR1-Mediated In Vivo Efficient Haploid Induction Service
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- Plant CNV Analysis Service
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BBM-BAR1-Mediated In Vivo Efficient Haploid Induction Service
INQUIRYTraditional haploid breeding methods, such as in vitro culture induction, are constrained by factors including strong genotype dependency, low induction rates, and complex tissue culture conditions, making them difficult to widely apply across diverse crops. In recent years, significant progress has been made in the development of novel in vivo haploid induction systems for model plants and major crops. Notably, the BBM and BAR1-mediated microspore fate reprogramming technology provides a robust molecular foundation for establishing a novel pathway enabling efficient in vivo haploid induction without exogenous tissue culture.
Technical principle
BBM (BABY BOOM) is a key AP2/ERF family transcription factor central to the induction of plant somatic embryogenesis. It regulates a series of downstream genes associated with embryogenesis, such as the LAFL gene family. Recent studies further reveal that its key downstream effector BAR1 can independently initiate microspore embryogenesis. Collectively, BBM and BAR1 form a potent regulatory module that precisely initiates the embryonic development program at the microspore stage.
Lifeasible stays at the forefront of plant research by establishing an advanced and reliable BBM-BAR1-mediated in vivo haploid induction technology platform. This enables researchers to directly reprogram the developmental fate of microspores from the gametophyte pathway to the embryophyte pathway within the plant itself (in vivo). Consequently, efficient haploid induction is achieved without external stress, significantly advancing plant breeding research.
Advantages of BBM-BAR1-Mediated In Vivo Efficient Haploid Induction
- Accelerate breeding processes. Through the BBM-BAR1-mediated in vivo efficient haploid induction technology, researchers can rapidly obtain homozygous DH lines, shortening the time required for new variety development.
- Enhance breeding efficiency and success rates. Compared to traditional mutagenesis techniques for obtaining haploid plants, this technology platform enables breeders to screen superior gene combinations within the same timeframe, significantly improving the overall throughput and success rates of breeding projects.
- Simplify breeding workflows and reduce overall costs. This technology eliminates reliance on complex in vitro culture and stress conditions, making the entire process more streamlined, stable, and standardized. While initial steps may involve gene editing or transformation, its high throughput and efficiency collectively lower screening costs and time per breeding material unit.
- Paves new breeding pathways. For crops where traditional haploid induction is extremely challenging, BBM-BAR1 technology opens an efficient new avenue for breeding.
Our Service for BBM-BAR1-Mediated In Vivo Efficient Haploid Induction
We have established a comprehensive in vivo haploid induction technology platform mediated by BBM-BAR1 for microspore fate reprogramming, provide the following core services to research institutions and breeding enterprises.
BBM-BAR1 module construction and optimization
Based on target crop genomic information, we design and optimize combinations of BBM, BAR1, and related regulatory elements (e.g., promoters, transcription activation domains).
Construct multiple expression vectors, including tissue-specific promoter-driven systems and inducible expression systems.
In vivo transformation of induction vectors and establishment of stable genetic lines
We establish transformation lines using highly efficient Agrobacterium-mediated or gene gun transformation technologies.
Through multi-generational screening, we obtain highly expressed and stably inherited induction lines, providing the material foundation for subsequent haploid induction experiments.
Haploid induction and phenotypic screening services
We conduct in vivo haploid embryo formation assays by crossing BBM-BAR1 induction lines with target materials or performing self-pollination experiments.
During this process, we rapidly identify haploid individuals using flow cytometry, chromosome counting, and molecular marker-assisted screening.
Gene expression and molecular mechanism validation
We provide multiple analysis platforms, including qRT-PCR, RNA-seq, and ATAC-seq, to examine transcriptional regulation patterns of the BBM-BAR1 module across developmental stages.
Additionally, we support clients in conducting related molecular mechanism studies and functional validation.
Our Service Process
- Project consultation and solution design. Collaborate with clients to understand their breeding objectives, target crops, and specific materials, jointly designing optimal technical solutions.
- Client material preparation and submission. Clients provide starting materials such as seeds, seedlings, or explants at specific growth stages according to standardized guidelines.
- Introduction of the BBM-BAR1 regulatory module. Using our advanced gene editing platform or efficient genetic transformation system, we precisely introduce the BBM-BAR1 expression cassette into client-provided materials. This step is critical for achieving in vivo induction.
- Regeneration and in vivo haploid induction. Transformed materials are regenerated into plants. During their development, the specific expression of the BBM-BAR1 module directly induces haploid embryo formation at the microspore stage.
- Haploid identification and chromosome doubling. Precisely screen haploid seedlings using morphological markers and flow cytometry, then employ efficient chromosome doubling techniques to generate fertile DH plants.
- DH line propagation and delivery. Propagate DH lines in standardized greenhouses or isolated facilities, harvest seeds, and deliver them to clients alongside detailed experimental reports.
Fig.2 Our service process. (Lifeasible).
Highlights of Our BBM-BAR1-Mediated In Vivo Efficient Haploid Induction Service
- Advanced service team. Our expert technical team possesses extensive service experience in plant molecular biology, gene editing, and modern breeding.
- Advanced technical platforms. Our laboratory is equipped with top-tier facilities, including a high-throughput gene editing platform, fully automated micromanipulation systems, and intelligent plant cultivation environments.
- End-to-end quality control (QC) system. From sequencing validation of vector constructs to molecular detection of transformation events and flow cytometry identification of haploid/polyploid levels, every step of our service adheres to stringent QC standards to ensure flawless delivery of results.
Lifeasible focuses on cutting-edge plant research. Our BBM-BAR1-mediated in vivo efficient haploid induction service aims to help researchers propel breeding projects toward breakthrough advancements. If you are interested, please feel free to contact us.
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