Services
- Genetic Marker Assisted Breeding
- Plant Molecular Biology Research
- Research on DNA Level of Plant
- Research on Microspore Embryogenesis
- Plant Genetic Engineering
- Plant Genome Editing with CRISPR / Cas9
- Plant Genome Editing with TALEN
- Plant Genome Editing with ZFN
- RNAi Mediated Plant Gene Silencing
- Overexpression of Plant Genes
- Arabidopsis thaliana Transformation
- Oryza sativa Transformation
- Zea mays Transformation
- Triticum aestivum Transformation
- Medicago truncatula Transformation
- Glycine max Transformation
- Gossypium hirsutum Transformation
- Nicotiana tabacum Transformation
- Solanum lycopersicum Transformation
- Brassica napus Transformation
- Solanum tuberosum Transformation
- Virus-Induced Gene Silencing (VIGS) of Plant Genes
- Research on Protein Level of Plant
- Plant Epigenetic Modification Testing Services
- Sequencing-based Plant Breeding
- Plant DNA-level Sequencing Services
- Plant Genome De Novo Service
- Plant Whole Genome Resequencing Service
- Plant Reduced-Representation Genome Sequencing (RRGS)
- Plant Genetic Map Service
- BSA Trait Positioning of Plant
- Genome-Wide Association Study (GWAS) of Plant
- eQTL Analysis of Plant
- Plant Genetic Evolution Service
- Plant Pan-genome Sequencing
- Plant Whole Exome Sequencing Service
- Individual Selection Pressure Analysis of Plant
- Mixing-tank Selection Pressure Analysis of Plant
- Plant Whole Genome Survey
- Plant RNA Level Sequencing Services
- Eukaryotic Transcriptome Sequencing without Reference Genome
- Eukaryotic Transcriptome Sequencing with Reference Genome
- Prokaryotic Transcriptome Sequencing Analysis
- LncRNA Sequencing of Plant
- Plant Small RNA Sequencing
- Plant Circular RNA Sequencing
- Plant Comparative Transcriptome Service
- Plant Isoform-sequencing with Reference Genome
- Plant Isoform-sequencing without Reference Genome
- Ribo-seq of Plant
- Metatranscriptome Sequencing of Plant
- Plant Single Cell Level Sequencing Services
- Plant Epigenetics Level Sequencing Services
- Plant Proteomics Service
- Proteomics Qualitative Analysis in Plant
- Plant Protein Quantitative Analysis Service Based on Isotope Labeling (iTRAQ / TMT)
- Non-labeled Plant Protein Quantitative Analysis (Label-free / DIA)
- Plant Protein Targeted Quantitative Service (PRM / MRM / AQUA)
- Post-translational Modification Proteomics (PTMs) Service for Plant
- Plant Metabolomics Services
- Plant DNA-level Sequencing Services
- Other Services
- Plant CNV Analysis Service
- Plant Mutation Detection Service
- Plant Strain / Cell Level Services
- Plant Tissue and Cell Culture Services
- Plant Polyploidization Services
- Plant Haploidization Services
- Plant Phenotypic Analysis
- Plant Stress Response Indicators Analysis
- Plant Biochemical Analysis
- Plant Tissue and Cell Imaging Services
- Plant Disease Identification Services
- Plant Organelle Isolation Services
- Genetically Modified Plant Testing Services
- Seed Testing Services
RIP-seq in Plant
INQUIRYIntroductions
RIP-seq is based on RNA-binding protein immunoprecipitation (RIP), which uses specific antibodies for co-immunoprecipitation of RNA-binding proteins. After precipitation, RNA is isolated, and through high-throughput sequencing, the binding of RNA and protein in the cell is analyzed in the whole transcriptome. RIP-seq can reveal the interaction between RNA molecules and RBP in the whole transcriptome, including the interaction between non-coding RNA and protein. It has been widely used in the research of human, mouse, rice, Arabidopsis and other species. The main applications in the field of agricultural molecular breeding are as follows: 1) Study the RNA region or type that a specific protein specifically binds. 2) Study the difference analysis of different protein binding RNA types. 3) Study the targeted regulatory genes of miRNA, etc.
Services
Lifeasible uses excellent RIP-seq technology, combined with cost-effective sequencing data and information analysis, to screen and identify protein binding sites across the entire transcriptome, systematically, comprehensively and accurately mine binding sites, and analyze target RNA in depth Species and their interactions with proteins.
Workflow of RIP-seq in plant
Analysis content
| Analysis content | Solved problem |
|---|---|
| Sequencing data quality assessment | Filter out low-quality data to ensure data quality |
| Align with reference genome | scc analysis to determine the effect of IP |
| Peak calling | Analyze protein binding sites |
| Correlation analysis between samples | Determine whether the experimental grouping design is reasonable |
| Motif analysis | Protein binding sequence preference |
| Peak-related gene annotation | Looking for genes that the protein potentially regulates or binds |
| Difference peak analysis | Analyze the difference between different samples peak |
| Functional analysis of related genes | GO / KEGG enrichment analysis of related genes |
Sample requirements
- For RNA captured by RIP, total amount > 500ng, concentration > 30 ng/μl, OD260/280 = 1.8~2.0, OD260/230 = 1.5~1.8.
Project cycle
- It takes 50 working days to complete the standard process.
Feedback to customers
- Raw data
- A project report includes experimental process, instrument parameters, analysis methods and results, etc.
Service process
Advantages
- Lifeasible specializes in providing services related to agricultural molecular breeding, with standardized systems and scientific management.
- The experiment adopts a carefully optimized experimental procedure, which has extremely high efficiency and specificity.
- With a strong bioinformatics team, it can meet the various in-depth data analysis needs of customers.
- The one-stop service we provide makes your upstream and downstream experiments seamlessly connected, facilitates your experiments more conveniently and quickly.
- Personalized customized package service.
※ It should be noted that our service is only used for research.
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